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A Preliminary Study of FTIR Spectroscopy as a Potential Non-Invasive Screening Tool for Pediatric Precursor B Lymphoblastic Leukemia

Early detection of the most common pediatric neoplasm, B-cell precursor lymphoblastic leukemia (BCP-ALL), is challenging and requires invasive bone marrow biopsies. The purpose of this study was to establish new biomarkers for early screening to detect pediatric leukemia. In this small cohort study,...

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Autores principales: Chaber, Radosław, Kowal, Aneta, Jakubczyk, Paweł, Arthur, Christopher, Łach, Kornelia, Wojnarowska-Nowak, Renata, Kusz, Krzysztof, Zawlik, Izabela, Paszek, Sylwia, Cebulski, Józef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926870/
https://www.ncbi.nlm.nih.gov/pubmed/33671817
http://dx.doi.org/10.3390/molecules26041174
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author Chaber, Radosław
Kowal, Aneta
Jakubczyk, Paweł
Arthur, Christopher
Łach, Kornelia
Wojnarowska-Nowak, Renata
Kusz, Krzysztof
Zawlik, Izabela
Paszek, Sylwia
Cebulski, Józef
author_facet Chaber, Radosław
Kowal, Aneta
Jakubczyk, Paweł
Arthur, Christopher
Łach, Kornelia
Wojnarowska-Nowak, Renata
Kusz, Krzysztof
Zawlik, Izabela
Paszek, Sylwia
Cebulski, Józef
author_sort Chaber, Radosław
collection PubMed
description Early detection of the most common pediatric neoplasm, B-cell precursor lymphoblastic leukemia (BCP-ALL), is challenging and requires invasive bone marrow biopsies. The purpose of this study was to establish new biomarkers for early screening to detect pediatric leukemia. In this small cohort study, Fourier transform infrared (FTIR) spectra were obtained from blood sera of 10 patients with BCP-ALL and were compared with the control samples from 10 children with some conditions other than neoplasm. Using various analytical approaches, including a new physical model, some significant differences were observable. The most important include: the different peak area ratio 2965/1645 cm(−1) (p = 0.002); the lower average percentage of both β-sheet and β-turn protein structures in the sera of BCP-ALL patients (p = 0.03); an AdaBoost-based predictive model for classifying healthy vs. BCP-ALL patients with 85% accuracy; and the phase shift of the first derivative in the spectral range 1050–1042 cm(−1) correlating with white blood cell (WBC) and blast cell count in BCP-ALL patients contrary to the samples obtained from healthy controls. Although verification in larger groups of patients will be necessary, these promising results suggest that FTIR spectroscopy may have future potential for the early screening of BCP-ALL.
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spelling pubmed-79268702021-03-04 A Preliminary Study of FTIR Spectroscopy as a Potential Non-Invasive Screening Tool for Pediatric Precursor B Lymphoblastic Leukemia Chaber, Radosław Kowal, Aneta Jakubczyk, Paweł Arthur, Christopher Łach, Kornelia Wojnarowska-Nowak, Renata Kusz, Krzysztof Zawlik, Izabela Paszek, Sylwia Cebulski, Józef Molecules Article Early detection of the most common pediatric neoplasm, B-cell precursor lymphoblastic leukemia (BCP-ALL), is challenging and requires invasive bone marrow biopsies. The purpose of this study was to establish new biomarkers for early screening to detect pediatric leukemia. In this small cohort study, Fourier transform infrared (FTIR) spectra were obtained from blood sera of 10 patients with BCP-ALL and were compared with the control samples from 10 children with some conditions other than neoplasm. Using various analytical approaches, including a new physical model, some significant differences were observable. The most important include: the different peak area ratio 2965/1645 cm(−1) (p = 0.002); the lower average percentage of both β-sheet and β-turn protein structures in the sera of BCP-ALL patients (p = 0.03); an AdaBoost-based predictive model for classifying healthy vs. BCP-ALL patients with 85% accuracy; and the phase shift of the first derivative in the spectral range 1050–1042 cm(−1) correlating with white blood cell (WBC) and blast cell count in BCP-ALL patients contrary to the samples obtained from healthy controls. Although verification in larger groups of patients will be necessary, these promising results suggest that FTIR spectroscopy may have future potential for the early screening of BCP-ALL. MDPI 2021-02-22 /pmc/articles/PMC7926870/ /pubmed/33671817 http://dx.doi.org/10.3390/molecules26041174 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chaber, Radosław
Kowal, Aneta
Jakubczyk, Paweł
Arthur, Christopher
Łach, Kornelia
Wojnarowska-Nowak, Renata
Kusz, Krzysztof
Zawlik, Izabela
Paszek, Sylwia
Cebulski, Józef
A Preliminary Study of FTIR Spectroscopy as a Potential Non-Invasive Screening Tool for Pediatric Precursor B Lymphoblastic Leukemia
title A Preliminary Study of FTIR Spectroscopy as a Potential Non-Invasive Screening Tool for Pediatric Precursor B Lymphoblastic Leukemia
title_full A Preliminary Study of FTIR Spectroscopy as a Potential Non-Invasive Screening Tool for Pediatric Precursor B Lymphoblastic Leukemia
title_fullStr A Preliminary Study of FTIR Spectroscopy as a Potential Non-Invasive Screening Tool for Pediatric Precursor B Lymphoblastic Leukemia
title_full_unstemmed A Preliminary Study of FTIR Spectroscopy as a Potential Non-Invasive Screening Tool for Pediatric Precursor B Lymphoblastic Leukemia
title_short A Preliminary Study of FTIR Spectroscopy as a Potential Non-Invasive Screening Tool for Pediatric Precursor B Lymphoblastic Leukemia
title_sort preliminary study of ftir spectroscopy as a potential non-invasive screening tool for pediatric precursor b lymphoblastic leukemia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926870/
https://www.ncbi.nlm.nih.gov/pubmed/33671817
http://dx.doi.org/10.3390/molecules26041174
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