Cargando…

Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos

SIMPLE SUMMARY: Liposome-mediated gene transfer has become an alternative method for establishing a gene targeting framework, and the production of mutant animals may be feasible even in laboratories without specialized equipment. However, whether blastocyst genome editing can be performed by treatm...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hirata, Maki, Wittayarat, Manita, Namula, Zhao, Anh Le, Quynh, Lin, Qingyi, Takebayashi, Koki, Thongkittidilok, Chommanart, Tanihara, Fuminori, Otoi, Takeshige
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926877/ https://www.ncbi.nlm.nih.gov/pubmed/33672168 http://dx.doi.org/10.3390/ani11020578

Ejemplares similares

Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events
por: Lin, Qingyi, et al.
Publicado: (2021)

Generation of mutant pigs by lipofection-mediated genome editing in embryos
por: Hirata, Maki, et al.
Publicado: (2021)

Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages
por: Le, Quynh Anh, et al.
Publicado: (2021)

Zona pellucida treatment before CRISPR/Cas9‐mediated genome editing of porcine zygotes
por: Namula, Zhao, et al.
Publicado: (2021)

Improvement of the in vitro fertilization and embryo development using frozen–thawed spermatozoa of microminipigs
por: Namula, Zhao, et al.
Publicado: (2021)

Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system
por: Namula, Zhao, et al.
Publicado: (2022)

Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection
por: Namula, Zhao, et al.
Publicado: (2022)

Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods
por: Le, Quynh Anh, et al.
Publicado: (2022)

The Relationship between Embryonic Development and the Efficiency of Target Mutations in Porcine Endogenous Retroviruses (PERVs) Pol Genes in Porcine Embryos
por: Hirata, Maki, et al.
Publicado: (2019)

Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN
por: Tanihara, Fuminori, et al.
Publicado: (2023)

Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos
por: TANIHARA, Fuminori, et al.
Publicado: (2019)

Mosaic TP53 Mutation on Tumour Development in Pigs: A Case Study
por: Thongkittidilok, Chommanart, et al.
Publicado: (2023)

One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis
por: Tanihara, Fuminori, et al.
Publicado: (2021)

Current status of the application of gene editing in pigs
por: TANIHARA, Fuminori, et al.
Publicado: (2021)

Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation
por: Tanihara, Fuminori, et al.
Publicado: (2018)

Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes
por: Tanihara, Fuminori, et al.
Publicado: (2020)

Effect of ferulic acid supplementation on the developmental competence of porcine embryos during in vitro maturation
por: TANIHARA, Fuminori, et al.
Publicado: (2018)

The optimal period of Ca-EDTA treatment for parthenogenetic activation of porcine oocytes during maturation culture
por: MORITA, Yasuhiro, et al.
Publicado: (2016)

Production of Genetically Modified Porcine Embryos via Lipofection of Zona-Pellucida-Intact Oocytes Using the CRISPR/Cas9 System
por: Piñeiro-Silva, Celia, et al.
Publicado: (2023)

The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts
por: TANIHARA, Fuminori, et al.
Publicado: (2019)

Follicular development of canine ovaries stimulated by a combination treatment of eCG and hCG
por: Hirata, Maki, et al.
Publicado: (2018)

Vaginal stimulation enhances ovulation of queen ovaries treated using a combination of eCG and hCG
por: Yoshimura, Naoaki, et al.
Publicado: (2021)

The Effects of an In Vitro Oocyte Maturation System and Chlorogenic Acid Supplementation during Embryo Culture on the Development of Porcine Cloned Embryos Derived from Native Vietnamese Ban Pigs
por: Nguyen, Thanh Van, et al.
Publicado: (2023)

Evaluation of Zona Pellucida Function for Sperm Penetration During In Vitro Fertilization in Pigs
por: TANIHARA, Fuminori, et al.
Publicado: (2013)

Epidermal growth factor improves developmental competence and embryonic quality of singly cultured domestic cat embryos
por: THONGKITTIDILOK, Chommanart, et al.
Publicado: (2015)

Protocol for generating monoclonal CRISPR-Cas9-mediated knockout cell lines using RNPs and lipofection in HNSCC cells
por: Geyer, Fabian, et al.
Publicado: (2023)

CRISPR/Cas9 technology: applications in oocytes and early embryos
por: Zhang, Yi-ran, et al.
Publicado: (2023)

Characterization and In Vitro Culture of Putative Spermatogonial Stem Cells Derived from Feline Testicular Tissue
por: TIPTANAVATTANA, Narong, et al.
Publicado: (2013)

In Vitro Fertilization and Development of Porcine Oocytes Matured in Follicular Fluid
por: AGUNG, Budiyanto, et al.
Publicado: (2013)

Bovine endometrium-derived cultured cells are suitable for lipofection
por: Shiokawa, Mai, et al.
Publicado: (2021)

Lipofection mediated transfection fails for sea urchin coelomocytes
por: Barela Hudgell, Megan A., et al.
Publicado: (2022)

Comparative Effects of Different Dosages of hCG on Follicular Development in Postpartum Dairy Cows With Cystic Ovarian Follicles
por: Ono, Tetsushi, et al.
Publicado: (2018)

Generation of Live Piglets from Cryopreserved Oocytes for the First Time Using a Defined System for In Vitro Embryo Production
por: Somfai, Tamás, et al.
Publicado: (2014)

Lipofection of Non-integrative CRISPR/Cas9 Ribonucleoproteins in Male Germline Stem Cells: A Simple and Effective Knockout Tool for Germline Genome Engineering
por: Obermeier, Mariella, et al.
Publicado: (2022)

Enrichment of bovine X-sperm using microfluidic dielectrophoretic chip: A proof-of- concept study
por: Wongtawan, Tuempong, et al.
Publicado: (2020)

Establishment of Lipofection for Studying miRNA Function in Human Adipocytes
por: Enlund, Eveliina, et al.
Publicado: (2014)

Live Cell Characterization of DNA Aggregation Delivered through Lipofection
por: Mieruszynski, Stephen, et al.
Publicado: (2015)

Lipofection of siRNA into bovine 8-16-cell stage embryos using zona removal and the well-of-the-well culture system
por: IKEDA, Shuntaro, et al.
Publicado: (2018)

Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer
por: Vancha, Ajith R, et al.
Publicado: (2004)

Lipofection with Synthetic mRNA as a Simple Method for T-Cell Immunomonitoring
por: Jarzebska, Natalia Teresa, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_p68nf91iv0f2f4qjbohhpcf7lh