Cargando…

Association of CX3CR1 Gene Polymorphisms with Fractalkine, Fractalkine Receptor, and C-Reactive Protein Levels in Patients with Kidney Failure

Fractalkine (CX3CL1) is a chemokine that plays a significant role in inflammation, one of the pathophysiological processes underlying end-stage renal disease (ESRD). Genetic factors are significantly involved in cytokine expression and have been studied as potential risk factors for chronic kidney d...

Descripción completa

Detalles Bibliográficos
Autores principales: Woźny, Łukasz, Żywiec, Joanna, Gosek, Katarzyna, Kuźniewicz, Roman, Górczyńska-Kosiorz, Sylwia, Trautsolt, Wanda, Śnit, Mirosław, Grzeszczak, Władysław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926901/
https://www.ncbi.nlm.nih.gov/pubmed/33672355
http://dx.doi.org/10.3390/ijerph18042202
_version_ 1783659568943857664
author Woźny, Łukasz
Żywiec, Joanna
Gosek, Katarzyna
Kuźniewicz, Roman
Górczyńska-Kosiorz, Sylwia
Trautsolt, Wanda
Śnit, Mirosław
Grzeszczak, Władysław
author_facet Woźny, Łukasz
Żywiec, Joanna
Gosek, Katarzyna
Kuźniewicz, Roman
Górczyńska-Kosiorz, Sylwia
Trautsolt, Wanda
Śnit, Mirosław
Grzeszczak, Władysław
author_sort Woźny, Łukasz
collection PubMed
description Fractalkine (CX3CL1) is a chemokine that plays a significant role in inflammation, one of the pathophysiological processes underlying end-stage renal disease (ESRD). Genetic factors are significantly involved in cytokine expression and have been studied as potential risk factors for chronic kidney disease (CKD). Objectives: We aimed to elucidate the association of CX3CR1 gene polymorphisms rs3732378 and rs3732379 with the levels of CX3CL1, CX3CL1 receptor (CX3CR1), as well as C-reactive protein (CRP). Patients and methods: We enrolled 198 participants, including 106 patients with ESRD and 92 controls. Peripheral blood samples were collected from each patient for genetic (rs3732378 and rs3732379 polymorphisms) and immunoenzymatic (fractalkine, CX3CR1, CRP) tests. Results: CX3CR1 and CRP levels were higher in patients with ESRD than in controls (p < 0.05). Fractalkine levels were significantly higher in ESRD patients who were homozygous for the G allele of the rs3732378 polymorphism and for the C allele of the rs3732379 polymorphism than in homozygous controls. Moreover, carriers of these alleles among patients with ESRD had significantly higher CX3CR1 levels than controls. Conclusions: The G allele of the rs3732378 polymorphism and the C allele of the rs3732379 polymorphism of the CX3CR1 gene are associated with higher CX3CL1 and CX3CR1 levels. Our study suggests that CX3CR1 gene polymorphisms could be potentially involved in the pathogenesis of ESRD, but the study needs to be replicated in a larger population with a longitudinal follow-up study. Identification of genetic factors associated with inflammation in ESRD may contribute to the development of targeted gene therapies in the future.
format Online
Article
Text
id pubmed-7926901
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79269012021-03-04 Association of CX3CR1 Gene Polymorphisms with Fractalkine, Fractalkine Receptor, and C-Reactive Protein Levels in Patients with Kidney Failure Woźny, Łukasz Żywiec, Joanna Gosek, Katarzyna Kuźniewicz, Roman Górczyńska-Kosiorz, Sylwia Trautsolt, Wanda Śnit, Mirosław Grzeszczak, Władysław Int J Environ Res Public Health Article Fractalkine (CX3CL1) is a chemokine that plays a significant role in inflammation, one of the pathophysiological processes underlying end-stage renal disease (ESRD). Genetic factors are significantly involved in cytokine expression and have been studied as potential risk factors for chronic kidney disease (CKD). Objectives: We aimed to elucidate the association of CX3CR1 gene polymorphisms rs3732378 and rs3732379 with the levels of CX3CL1, CX3CL1 receptor (CX3CR1), as well as C-reactive protein (CRP). Patients and methods: We enrolled 198 participants, including 106 patients with ESRD and 92 controls. Peripheral blood samples were collected from each patient for genetic (rs3732378 and rs3732379 polymorphisms) and immunoenzymatic (fractalkine, CX3CR1, CRP) tests. Results: CX3CR1 and CRP levels were higher in patients with ESRD than in controls (p < 0.05). Fractalkine levels were significantly higher in ESRD patients who were homozygous for the G allele of the rs3732378 polymorphism and for the C allele of the rs3732379 polymorphism than in homozygous controls. Moreover, carriers of these alleles among patients with ESRD had significantly higher CX3CR1 levels than controls. Conclusions: The G allele of the rs3732378 polymorphism and the C allele of the rs3732379 polymorphism of the CX3CR1 gene are associated with higher CX3CL1 and CX3CR1 levels. Our study suggests that CX3CR1 gene polymorphisms could be potentially involved in the pathogenesis of ESRD, but the study needs to be replicated in a larger population with a longitudinal follow-up study. Identification of genetic factors associated with inflammation in ESRD may contribute to the development of targeted gene therapies in the future. MDPI 2021-02-23 2021-02 /pmc/articles/PMC7926901/ /pubmed/33672355 http://dx.doi.org/10.3390/ijerph18042202 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Woźny, Łukasz
Żywiec, Joanna
Gosek, Katarzyna
Kuźniewicz, Roman
Górczyńska-Kosiorz, Sylwia
Trautsolt, Wanda
Śnit, Mirosław
Grzeszczak, Władysław
Association of CX3CR1 Gene Polymorphisms with Fractalkine, Fractalkine Receptor, and C-Reactive Protein Levels in Patients with Kidney Failure
title Association of CX3CR1 Gene Polymorphisms with Fractalkine, Fractalkine Receptor, and C-Reactive Protein Levels in Patients with Kidney Failure
title_full Association of CX3CR1 Gene Polymorphisms with Fractalkine, Fractalkine Receptor, and C-Reactive Protein Levels in Patients with Kidney Failure
title_fullStr Association of CX3CR1 Gene Polymorphisms with Fractalkine, Fractalkine Receptor, and C-Reactive Protein Levels in Patients with Kidney Failure
title_full_unstemmed Association of CX3CR1 Gene Polymorphisms with Fractalkine, Fractalkine Receptor, and C-Reactive Protein Levels in Patients with Kidney Failure
title_short Association of CX3CR1 Gene Polymorphisms with Fractalkine, Fractalkine Receptor, and C-Reactive Protein Levels in Patients with Kidney Failure
title_sort association of cx3cr1 gene polymorphisms with fractalkine, fractalkine receptor, and c-reactive protein levels in patients with kidney failure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926901/
https://www.ncbi.nlm.nih.gov/pubmed/33672355
http://dx.doi.org/10.3390/ijerph18042202
work_keys_str_mv AT woznyłukasz associationofcx3cr1genepolymorphismswithfractalkinefractalkinereceptorandcreactiveproteinlevelsinpatientswithkidneyfailure
AT zywiecjoanna associationofcx3cr1genepolymorphismswithfractalkinefractalkinereceptorandcreactiveproteinlevelsinpatientswithkidneyfailure
AT gosekkatarzyna associationofcx3cr1genepolymorphismswithfractalkinefractalkinereceptorandcreactiveproteinlevelsinpatientswithkidneyfailure
AT kuzniewiczroman associationofcx3cr1genepolymorphismswithfractalkinefractalkinereceptorandcreactiveproteinlevelsinpatientswithkidneyfailure
AT gorczynskakosiorzsylwia associationofcx3cr1genepolymorphismswithfractalkinefractalkinereceptorandcreactiveproteinlevelsinpatientswithkidneyfailure
AT trautsoltwanda associationofcx3cr1genepolymorphismswithfractalkinefractalkinereceptorandcreactiveproteinlevelsinpatientswithkidneyfailure
AT snitmirosław associationofcx3cr1genepolymorphismswithfractalkinefractalkinereceptorandcreactiveproteinlevelsinpatientswithkidneyfailure
AT grzeszczakwładysław associationofcx3cr1genepolymorphismswithfractalkinefractalkinereceptorandcreactiveproteinlevelsinpatientswithkidneyfailure