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Competitive Exclusion of Intra-Genus Salmonella in Neonatal Broilers

Salmonellosis is a zoonotic infection caused by Salmonella enterica serotypes contracted from contaminated products. We hypothesized that competitive exclusion between Salmonella serotypes in neonatal broilers would reduce colonization and affect the host immune response. Day of hatch broilers were...

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Autores principales: Pineda, Megan, Kogut, Michael, Genovese, Kenneth, Farnell, Yuhua Z., Zhao, Dan, Wang, Xi, Milby, Allison, Farnell, Morgan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926974/
https://www.ncbi.nlm.nih.gov/pubmed/33670039
http://dx.doi.org/10.3390/microorganisms9020446
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author Pineda, Megan
Kogut, Michael
Genovese, Kenneth
Farnell, Yuhua Z.
Zhao, Dan
Wang, Xi
Milby, Allison
Farnell, Morgan
author_facet Pineda, Megan
Kogut, Michael
Genovese, Kenneth
Farnell, Yuhua Z.
Zhao, Dan
Wang, Xi
Milby, Allison
Farnell, Morgan
author_sort Pineda, Megan
collection PubMed
description Salmonellosis is a zoonotic infection caused by Salmonella enterica serotypes contracted from contaminated products. We hypothesized that competitive exclusion between Salmonella serotypes in neonatal broilers would reduce colonization and affect the host immune response. Day of hatch broilers were randomly allocated to one of six treatment groups: (1) control, which received saline, (2) Salmonella Kentucky (SK) only on day 1 (D1), (3) Salmonella Typhimurium (ST) or Salmonella Enteritidis (SE) only on D1, (4) SK on D1 then ST or SE on day 2 (D2), (5) ST or SE on D1 then SK on D2, and (6) SK and ST or SE concurrently on D1. Salmonella gut colonization and incidence were measured from cecal contents. Livers and spleens were combined and macerated to determine systemic translocation. Relative mRNA levels of interleukin-1β (IL-1β), IL-6, IL-10, IL-18, and gamma interferon (IFN-γ) were measured in cecal tonsils and liver to investigate local and systemic immune responses. When a serotype was administered first, it was able to significantly reduce colonization of the following serotype. Significant changes were found in mRNA expression of cytokines. These results suggest competitive exclusion by Salmonella enterica serotypes affect local and systemic immune responses.
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spelling pubmed-79269742021-03-04 Competitive Exclusion of Intra-Genus Salmonella in Neonatal Broilers Pineda, Megan Kogut, Michael Genovese, Kenneth Farnell, Yuhua Z. Zhao, Dan Wang, Xi Milby, Allison Farnell, Morgan Microorganisms Article Salmonellosis is a zoonotic infection caused by Salmonella enterica serotypes contracted from contaminated products. We hypothesized that competitive exclusion between Salmonella serotypes in neonatal broilers would reduce colonization and affect the host immune response. Day of hatch broilers were randomly allocated to one of six treatment groups: (1) control, which received saline, (2) Salmonella Kentucky (SK) only on day 1 (D1), (3) Salmonella Typhimurium (ST) or Salmonella Enteritidis (SE) only on D1, (4) SK on D1 then ST or SE on day 2 (D2), (5) ST or SE on D1 then SK on D2, and (6) SK and ST or SE concurrently on D1. Salmonella gut colonization and incidence were measured from cecal contents. Livers and spleens were combined and macerated to determine systemic translocation. Relative mRNA levels of interleukin-1β (IL-1β), IL-6, IL-10, IL-18, and gamma interferon (IFN-γ) were measured in cecal tonsils and liver to investigate local and systemic immune responses. When a serotype was administered first, it was able to significantly reduce colonization of the following serotype. Significant changes were found in mRNA expression of cytokines. These results suggest competitive exclusion by Salmonella enterica serotypes affect local and systemic immune responses. MDPI 2021-02-21 /pmc/articles/PMC7926974/ /pubmed/33670039 http://dx.doi.org/10.3390/microorganisms9020446 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pineda, Megan
Kogut, Michael
Genovese, Kenneth
Farnell, Yuhua Z.
Zhao, Dan
Wang, Xi
Milby, Allison
Farnell, Morgan
Competitive Exclusion of Intra-Genus Salmonella in Neonatal Broilers
title Competitive Exclusion of Intra-Genus Salmonella in Neonatal Broilers
title_full Competitive Exclusion of Intra-Genus Salmonella in Neonatal Broilers
title_fullStr Competitive Exclusion of Intra-Genus Salmonella in Neonatal Broilers
title_full_unstemmed Competitive Exclusion of Intra-Genus Salmonella in Neonatal Broilers
title_short Competitive Exclusion of Intra-Genus Salmonella in Neonatal Broilers
title_sort competitive exclusion of intra-genus salmonella in neonatal broilers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926974/
https://www.ncbi.nlm.nih.gov/pubmed/33670039
http://dx.doi.org/10.3390/microorganisms9020446
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