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On the Prevalence and Potential Functionality of an Intrinsic Disorder in the MERS-CoV Proteome

Middle East respiratory syndrome is a severe respiratory illness caused by an infectious coronavirus. This virus is associated with a high mortality rate, but there is as of yet no effective vaccine or antibody available for human immunity/treatment. Drug design relies on understanding the 3D struct...

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Autores principales: Alshehri, Manal A., Manee, Manee M., Alqahtani, Fahad H., Al-Shomrani, Badr M., Uversky, Vladimir N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926987/
https://www.ncbi.nlm.nih.gov/pubmed/33671602
http://dx.doi.org/10.3390/v13020339
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author Alshehri, Manal A.
Manee, Manee M.
Alqahtani, Fahad H.
Al-Shomrani, Badr M.
Uversky, Vladimir N.
author_facet Alshehri, Manal A.
Manee, Manee M.
Alqahtani, Fahad H.
Al-Shomrani, Badr M.
Uversky, Vladimir N.
author_sort Alshehri, Manal A.
collection PubMed
description Middle East respiratory syndrome is a severe respiratory illness caused by an infectious coronavirus. This virus is associated with a high mortality rate, but there is as of yet no effective vaccine or antibody available for human immunity/treatment. Drug design relies on understanding the 3D structures of viral proteins; however, arriving at such understanding is difficult for intrinsically disordered proteins, whose disorder-dependent functions are key to the virus’s biology. Disorder is suggested to provide viral proteins with highly flexible structures and diverse functions that are utilized when invading host organisms and adjusting to new habitats. To date, the functional roles of intrinsically disordered proteins in the mechanisms of MERS-CoV pathogenesis, transmission, and treatment remain unclear. In this study, we performed structural analysis to evaluate the abundance of intrinsic disorder in the MERS-CoV proteome and in individual proteins derived from the MERS-CoV genome. Moreover, we detected disordered protein binding regions, namely, molecular recognition features and short linear motifs. Studying disordered proteins/regions in MERS-CoV could contribute to unlocking the complex riddles of viral infection, exploitation strategies, and drug development approaches in the near future by making it possible to target these important (yet challenging) unstructured regions.
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spelling pubmed-79269872021-03-04 On the Prevalence and Potential Functionality of an Intrinsic Disorder in the MERS-CoV Proteome Alshehri, Manal A. Manee, Manee M. Alqahtani, Fahad H. Al-Shomrani, Badr M. Uversky, Vladimir N. Viruses Article Middle East respiratory syndrome is a severe respiratory illness caused by an infectious coronavirus. This virus is associated with a high mortality rate, but there is as of yet no effective vaccine or antibody available for human immunity/treatment. Drug design relies on understanding the 3D structures of viral proteins; however, arriving at such understanding is difficult for intrinsically disordered proteins, whose disorder-dependent functions are key to the virus’s biology. Disorder is suggested to provide viral proteins with highly flexible structures and diverse functions that are utilized when invading host organisms and adjusting to new habitats. To date, the functional roles of intrinsically disordered proteins in the mechanisms of MERS-CoV pathogenesis, transmission, and treatment remain unclear. In this study, we performed structural analysis to evaluate the abundance of intrinsic disorder in the MERS-CoV proteome and in individual proteins derived from the MERS-CoV genome. Moreover, we detected disordered protein binding regions, namely, molecular recognition features and short linear motifs. Studying disordered proteins/regions in MERS-CoV could contribute to unlocking the complex riddles of viral infection, exploitation strategies, and drug development approaches in the near future by making it possible to target these important (yet challenging) unstructured regions. MDPI 2021-02-22 /pmc/articles/PMC7926987/ /pubmed/33671602 http://dx.doi.org/10.3390/v13020339 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alshehri, Manal A.
Manee, Manee M.
Alqahtani, Fahad H.
Al-Shomrani, Badr M.
Uversky, Vladimir N.
On the Prevalence and Potential Functionality of an Intrinsic Disorder in the MERS-CoV Proteome
title On the Prevalence and Potential Functionality of an Intrinsic Disorder in the MERS-CoV Proteome
title_full On the Prevalence and Potential Functionality of an Intrinsic Disorder in the MERS-CoV Proteome
title_fullStr On the Prevalence and Potential Functionality of an Intrinsic Disorder in the MERS-CoV Proteome
title_full_unstemmed On the Prevalence and Potential Functionality of an Intrinsic Disorder in the MERS-CoV Proteome
title_short On the Prevalence and Potential Functionality of an Intrinsic Disorder in the MERS-CoV Proteome
title_sort on the prevalence and potential functionality of an intrinsic disorder in the mers-cov proteome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926987/
https://www.ncbi.nlm.nih.gov/pubmed/33671602
http://dx.doi.org/10.3390/v13020339
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