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IoT-Based Research Equipment Sharing System for Remotely Controlled Two-Photon Laser Scanning Microscopy

In this study, two-photon laser scanning microscopy (TPLSM) based on the internet of things (IoT) is proposed as a remote research equipment sharing system, which enables the remote sharing economy. IoT modules, where data are transmitted to and received from the remote users in the web service via...

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Autores principales: Park, Eunwoo, Lim, Jaehyun, Park, Byung Cheol, Kim, Daekeun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927017/
https://www.ncbi.nlm.nih.gov/pubmed/33672147
http://dx.doi.org/10.3390/s21041533
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author Park, Eunwoo
Lim, Jaehyun
Park, Byung Cheol
Kim, Daekeun
author_facet Park, Eunwoo
Lim, Jaehyun
Park, Byung Cheol
Kim, Daekeun
author_sort Park, Eunwoo
collection PubMed
description In this study, two-photon laser scanning microscopy (TPLSM) based on the internet of things (IoT) is proposed as a remote research equipment sharing system, which enables the remote sharing economy. IoT modules, where data are transmitted to and received from the remote users in the web service via IoT, instead of a data acquisition (DAQ) system embedded in the conventional TPLSM, are installed in the IoT-based TPLSM (IoT-TPLSM). The performance for each IoT module is evaluated independently, and it is confirmed that it works well even in a personal computer-free environment. In addition, a message queuing telemetry transport (MQTT) protocol is applied to the DAQ interface in the web service, and a graphic user interface for enabling the remote users to operate IoT-TPLSM remotely is also designed and implemented. For the image acquisition demonstration, the stained cellular images and the autofluorescent tissue images are obtained in IoT-TPLSM. Lastly, it is confirmed that the comparable performance is provided with the conventional TPLSM by evaluating the imaging conditions and qualities of the three-dimensional image stacks processed in IoT-TPLSM.
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spelling pubmed-79270172021-03-04 IoT-Based Research Equipment Sharing System for Remotely Controlled Two-Photon Laser Scanning Microscopy Park, Eunwoo Lim, Jaehyun Park, Byung Cheol Kim, Daekeun Sensors (Basel) Article In this study, two-photon laser scanning microscopy (TPLSM) based on the internet of things (IoT) is proposed as a remote research equipment sharing system, which enables the remote sharing economy. IoT modules, where data are transmitted to and received from the remote users in the web service via IoT, instead of a data acquisition (DAQ) system embedded in the conventional TPLSM, are installed in the IoT-based TPLSM (IoT-TPLSM). The performance for each IoT module is evaluated independently, and it is confirmed that it works well even in a personal computer-free environment. In addition, a message queuing telemetry transport (MQTT) protocol is applied to the DAQ interface in the web service, and a graphic user interface for enabling the remote users to operate IoT-TPLSM remotely is also designed and implemented. For the image acquisition demonstration, the stained cellular images and the autofluorescent tissue images are obtained in IoT-TPLSM. Lastly, it is confirmed that the comparable performance is provided with the conventional TPLSM by evaluating the imaging conditions and qualities of the three-dimensional image stacks processed in IoT-TPLSM. MDPI 2021-02-23 /pmc/articles/PMC7927017/ /pubmed/33672147 http://dx.doi.org/10.3390/s21041533 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Eunwoo
Lim, Jaehyun
Park, Byung Cheol
Kim, Daekeun
IoT-Based Research Equipment Sharing System for Remotely Controlled Two-Photon Laser Scanning Microscopy
title IoT-Based Research Equipment Sharing System for Remotely Controlled Two-Photon Laser Scanning Microscopy
title_full IoT-Based Research Equipment Sharing System for Remotely Controlled Two-Photon Laser Scanning Microscopy
title_fullStr IoT-Based Research Equipment Sharing System for Remotely Controlled Two-Photon Laser Scanning Microscopy
title_full_unstemmed IoT-Based Research Equipment Sharing System for Remotely Controlled Two-Photon Laser Scanning Microscopy
title_short IoT-Based Research Equipment Sharing System for Remotely Controlled Two-Photon Laser Scanning Microscopy
title_sort iot-based research equipment sharing system for remotely controlled two-photon laser scanning microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927017/
https://www.ncbi.nlm.nih.gov/pubmed/33672147
http://dx.doi.org/10.3390/s21041533
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