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A Micromachined Picocalorimeter Sensor for Liquid Samples with Application to Chemical Reactions and Biochemistry
Calorimetry has long been used to probe the physical state of a system by measuring the heat exchanged with the environment as a result of chemical reactions or phase transitions. Application of calorimetry to microscale biological samples, however, is hampered by insufficient sensitivity and the di...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927623/ https://www.ncbi.nlm.nih.gov/pubmed/33717854 http://dx.doi.org/10.1002/advs.202003415 |
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author | Bae, Jinhye Zheng, Juanjuan Zhang, Haitao Foster, Peter J. Needleman, Daniel J. Vlassak, Joost J. |
author_facet | Bae, Jinhye Zheng, Juanjuan Zhang, Haitao Foster, Peter J. Needleman, Daniel J. Vlassak, Joost J. |
author_sort | Bae, Jinhye |
collection | PubMed |
description | Calorimetry has long been used to probe the physical state of a system by measuring the heat exchanged with the environment as a result of chemical reactions or phase transitions. Application of calorimetry to microscale biological samples, however, is hampered by insufficient sensitivity and the difficulty of handling liquid samples at this scale. Here, a micromachined calorimeter sensor that is capable of resolving picowatt levels of power is described. The sensor consists of low‐noise thermopiles on a thin silicon nitride membrane that allow direct differential temperature measurements between a sample and four coplanar references, which significantly reduces thermal drift. The partial pressure of water in the ambient around the sample is maintained at saturation level using a small hydrogel‐lined enclosure. The materials used in the sensor and its geometry are optimized to minimize the noise equivalent power generated by the sensor in response to the temperature field that develops around a typical sample. The experimental response of the sensor is characterized as a function of thermopile dimensions and sample volume, and its capability is demonstrated by measuring the heat dissipated during an enzymatically catalyzed biochemical reaction in a microliter‐sized liquid droplet. The sensor offers particular promise for quantitative measurements on biological systems. |
format | Online Article Text |
id | pubmed-7927623 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79276232021-03-12 A Micromachined Picocalorimeter Sensor for Liquid Samples with Application to Chemical Reactions and Biochemistry Bae, Jinhye Zheng, Juanjuan Zhang, Haitao Foster, Peter J. Needleman, Daniel J. Vlassak, Joost J. Adv Sci (Weinh) Full Papers Calorimetry has long been used to probe the physical state of a system by measuring the heat exchanged with the environment as a result of chemical reactions or phase transitions. Application of calorimetry to microscale biological samples, however, is hampered by insufficient sensitivity and the difficulty of handling liquid samples at this scale. Here, a micromachined calorimeter sensor that is capable of resolving picowatt levels of power is described. The sensor consists of low‐noise thermopiles on a thin silicon nitride membrane that allow direct differential temperature measurements between a sample and four coplanar references, which significantly reduces thermal drift. The partial pressure of water in the ambient around the sample is maintained at saturation level using a small hydrogel‐lined enclosure. The materials used in the sensor and its geometry are optimized to minimize the noise equivalent power generated by the sensor in response to the temperature field that develops around a typical sample. The experimental response of the sensor is characterized as a function of thermopile dimensions and sample volume, and its capability is demonstrated by measuring the heat dissipated during an enzymatically catalyzed biochemical reaction in a microliter‐sized liquid droplet. The sensor offers particular promise for quantitative measurements on biological systems. John Wiley and Sons Inc. 2021-01-12 /pmc/articles/PMC7927623/ /pubmed/33717854 http://dx.doi.org/10.1002/advs.202003415 Text en © 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Bae, Jinhye Zheng, Juanjuan Zhang, Haitao Foster, Peter J. Needleman, Daniel J. Vlassak, Joost J. A Micromachined Picocalorimeter Sensor for Liquid Samples with Application to Chemical Reactions and Biochemistry |
title | A Micromachined Picocalorimeter Sensor for Liquid Samples with Application to Chemical Reactions and Biochemistry |
title_full | A Micromachined Picocalorimeter Sensor for Liquid Samples with Application to Chemical Reactions and Biochemistry |
title_fullStr | A Micromachined Picocalorimeter Sensor for Liquid Samples with Application to Chemical Reactions and Biochemistry |
title_full_unstemmed | A Micromachined Picocalorimeter Sensor for Liquid Samples with Application to Chemical Reactions and Biochemistry |
title_short | A Micromachined Picocalorimeter Sensor for Liquid Samples with Application to Chemical Reactions and Biochemistry |
title_sort | micromachined picocalorimeter sensor for liquid samples with application to chemical reactions and biochemistry |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927623/ https://www.ncbi.nlm.nih.gov/pubmed/33717854 http://dx.doi.org/10.1002/advs.202003415 |
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