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Isorhamnetin Inhibits Human Gallbladder Cancer Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway Inactivation

Gallbladder cancer (GBC) is the most common biliary tract tumor with a poor prognosis. Isorhamnetin is a flavonoid compound extracted from Hippophae rhamnoides L. and has several pharmacological effects including anti-inflammatory and anti-cancer properties. We treated GBC-SD and NOZ of GBC cell lin...

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Autores principales: Zhai, Tianyu, Zhang, Xiaoyu, Hei, Zhenyu, Jin, Longyang, Han, Chao, Ko, Audrey Tsznam, Yu, Xiaofeng, Wang, Jiandong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927673/
https://www.ncbi.nlm.nih.gov/pubmed/33679411
http://dx.doi.org/10.3389/fphar.2021.628621
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author Zhai, Tianyu
Zhang, Xiaoyu
Hei, Zhenyu
Jin, Longyang
Han, Chao
Ko, Audrey Tsznam
Yu, Xiaofeng
Wang, Jiandong
author_facet Zhai, Tianyu
Zhang, Xiaoyu
Hei, Zhenyu
Jin, Longyang
Han, Chao
Ko, Audrey Tsznam
Yu, Xiaofeng
Wang, Jiandong
author_sort Zhai, Tianyu
collection PubMed
description Gallbladder cancer (GBC) is the most common biliary tract tumor with a poor prognosis. Isorhamnetin is a flavonoid compound extracted from Hippophae rhamnoides L. and has several pharmacological effects including anti-inflammatory and anti-cancer properties. We treated GBC-SD and NOZ of GBC cell lines with different isorhamnetin concentrations in vitro. A cell counting kit-8 (CCK-8) assay, transwell assay, Hoechst 33342 stain assay, flow cytometric analysis, and a colony-forming assay were performed to investigate the effect of isorhamnetin on the proliferation, apoptosis, metastasis, and cycle arrest of GBC cells. A western blotting assay was conducted to explore the related protein expression level of GBC cells. A mice xenograft model and immunohistochemistry staining were employed to assess the effect of isorhamnetin in vivo. Isorhamnetin was found to suppress cell proliferation and metastasis, and trigger apoptosis and arrest the G2/M phase in GBC cells via the inactivation of the PI3K/AKT signaling cascade. Our findings are of clinical significance in providing a novel treatment approach for GBC.
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spelling pubmed-79276732021-03-04 Isorhamnetin Inhibits Human Gallbladder Cancer Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway Inactivation Zhai, Tianyu Zhang, Xiaoyu Hei, Zhenyu Jin, Longyang Han, Chao Ko, Audrey Tsznam Yu, Xiaofeng Wang, Jiandong Front Pharmacol Pharmacology Gallbladder cancer (GBC) is the most common biliary tract tumor with a poor prognosis. Isorhamnetin is a flavonoid compound extracted from Hippophae rhamnoides L. and has several pharmacological effects including anti-inflammatory and anti-cancer properties. We treated GBC-SD and NOZ of GBC cell lines with different isorhamnetin concentrations in vitro. A cell counting kit-8 (CCK-8) assay, transwell assay, Hoechst 33342 stain assay, flow cytometric analysis, and a colony-forming assay were performed to investigate the effect of isorhamnetin on the proliferation, apoptosis, metastasis, and cycle arrest of GBC cells. A western blotting assay was conducted to explore the related protein expression level of GBC cells. A mice xenograft model and immunohistochemistry staining were employed to assess the effect of isorhamnetin in vivo. Isorhamnetin was found to suppress cell proliferation and metastasis, and trigger apoptosis and arrest the G2/M phase in GBC cells via the inactivation of the PI3K/AKT signaling cascade. Our findings are of clinical significance in providing a novel treatment approach for GBC. Frontiers Media S.A. 2021-02-17 /pmc/articles/PMC7927673/ /pubmed/33679411 http://dx.doi.org/10.3389/fphar.2021.628621 Text en Copyright © 2021 Zhai, Zhang, Hei, Jin, Han, Ko, Yu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Zhai, Tianyu
Zhang, Xiaoyu
Hei, Zhenyu
Jin, Longyang
Han, Chao
Ko, Audrey Tsznam
Yu, Xiaofeng
Wang, Jiandong
Isorhamnetin Inhibits Human Gallbladder Cancer Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway Inactivation
title Isorhamnetin Inhibits Human Gallbladder Cancer Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway Inactivation
title_full Isorhamnetin Inhibits Human Gallbladder Cancer Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway Inactivation
title_fullStr Isorhamnetin Inhibits Human Gallbladder Cancer Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway Inactivation
title_full_unstemmed Isorhamnetin Inhibits Human Gallbladder Cancer Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway Inactivation
title_short Isorhamnetin Inhibits Human Gallbladder Cancer Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway Inactivation
title_sort isorhamnetin inhibits human gallbladder cancer cell proliferation and metastasis via pi3k/akt signaling pathway inactivation
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927673/
https://www.ncbi.nlm.nih.gov/pubmed/33679411
http://dx.doi.org/10.3389/fphar.2021.628621
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