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Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor

Despite the unprecedented gene editing capability of CRISPR-Cas9-mediated targeted knock-in, the efficiency and precision of this technology still require further optimization, particularly for multicellular model organisms, such as the zebrafish (Danio rerio). Our study demonstrated that an ∼200 ba...

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Detalles Bibliográficos
Autores principales:	Ranawakage, Deshani C., Okada, Keita, Sugio, Kota, Kawaguchi, Yuya, Kuninobu-Bonkohara, Yuki, Takada, Takuya, Kamachi, Yusuke
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Cell and Developmental Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928300/ https://www.ncbi.nlm.nih.gov/pubmed/33681181 http://dx.doi.org/10.3389/fcell.2020.598634

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