Cargando…

Exosomal miR-590-5p in Serum as a Biomarker for the Diagnosis and Prognosis of Gastric Cancer

The purpose of this study is to explore the expression of miRNA-590-5p, an exosome of gastric cancer (GC), and to evaluate the suitability of miR-590-5p, an exosome with its own clinical characteristics. Serum samples from 168 gastric cancer patients and 50 matched controls were collected and exosom...

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Guo-Dian, Xu, Zhi-Yuan, Hu, Can, Lv, Hang, Xie, Hua-Xia, Huang, Ting, Zhang, Yan-Qiang, Chen, Gui-Ping, Fu, Yu-Fei, Cheng, Xiang-Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928302/
https://www.ncbi.nlm.nih.gov/pubmed/33681295
http://dx.doi.org/10.3389/fmolb.2021.636566
_version_ 1783659825741168640
author Zheng, Guo-Dian
Xu, Zhi-Yuan
Hu, Can
Lv, Hang
Xie, Hua-Xia
Huang, Ting
Zhang, Yan-Qiang
Chen, Gui-Ping
Fu, Yu-Fei
Cheng, Xiang-Dong
author_facet Zheng, Guo-Dian
Xu, Zhi-Yuan
Hu, Can
Lv, Hang
Xie, Hua-Xia
Huang, Ting
Zhang, Yan-Qiang
Chen, Gui-Ping
Fu, Yu-Fei
Cheng, Xiang-Dong
author_sort Zheng, Guo-Dian
collection PubMed
description The purpose of this study is to explore the expression of miRNA-590-5p, an exosome of gastric cancer (GC), and to evaluate the suitability of miR-590-5p, an exosome with its own clinical characteristics. Serum samples from 168 gastric cancer patients and 50 matched controls were collected and exosomal RNAs were extracted. After that, miR-590-5p is analyzed by quantitative polymerase chain reaction (qRT-PCR), which is more related to clinical and pathological parameters and patient monitoring data. MGC-803 and HGC-27 cells were treated by miR-590-5p mimics, and then the changes of cell fluidity and invasiveness were monitored. The results showed that the expression level of miR-590-5p in exosomes of healthy observation group, early (I and II) stage group, and late stage (III) group was 30.34 ± 6.35, 6.19 ± 0.81, and 2.9 ± 0.19, respectively (all p < 0.05). ROC (receiver-operating characteristic curve) showed that the AUC (area under the curve) of exosomal miR-590-5p was 0.810 with 63.7% sensitivity and 86% specificity. The expression of exosomal miR-590-5p in serum was related to clinical stage (p = 0.008), infiltration depth, and the expression level of ki-67 (p < 0.001). In addition, Kaplan-Meier analysis showed that the decrease of explicit level of exosomal miR-590-5p was related to the decrease of overall survival rate (p < 0.001). Cox regression analysis showed that miR-590-5p can be used as an independent predictor. Furthermore, upregulation of miR-590-5p inhibited cell migration and invasion in MGC-803 cells and HGC-27 cells. The serum expression level of exosomal miR-590-5p may be a biomarker, which is potentially useful and noninvasive for early detection and prediction of GC. In addition, miR-590-5p can play a role in eliminating carcinogens by actively regulating the malignant potential of gastric cancer.
format Online
Article
Text
id pubmed-7928302
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-79283022021-03-04 Exosomal miR-590-5p in Serum as a Biomarker for the Diagnosis and Prognosis of Gastric Cancer Zheng, Guo-Dian Xu, Zhi-Yuan Hu, Can Lv, Hang Xie, Hua-Xia Huang, Ting Zhang, Yan-Qiang Chen, Gui-Ping Fu, Yu-Fei Cheng, Xiang-Dong Front Mol Biosci Molecular Biosciences The purpose of this study is to explore the expression of miRNA-590-5p, an exosome of gastric cancer (GC), and to evaluate the suitability of miR-590-5p, an exosome with its own clinical characteristics. Serum samples from 168 gastric cancer patients and 50 matched controls were collected and exosomal RNAs were extracted. After that, miR-590-5p is analyzed by quantitative polymerase chain reaction (qRT-PCR), which is more related to clinical and pathological parameters and patient monitoring data. MGC-803 and HGC-27 cells were treated by miR-590-5p mimics, and then the changes of cell fluidity and invasiveness were monitored. The results showed that the expression level of miR-590-5p in exosomes of healthy observation group, early (I and II) stage group, and late stage (III) group was 30.34 ± 6.35, 6.19 ± 0.81, and 2.9 ± 0.19, respectively (all p < 0.05). ROC (receiver-operating characteristic curve) showed that the AUC (area under the curve) of exosomal miR-590-5p was 0.810 with 63.7% sensitivity and 86% specificity. The expression of exosomal miR-590-5p in serum was related to clinical stage (p = 0.008), infiltration depth, and the expression level of ki-67 (p < 0.001). In addition, Kaplan-Meier analysis showed that the decrease of explicit level of exosomal miR-590-5p was related to the decrease of overall survival rate (p < 0.001). Cox regression analysis showed that miR-590-5p can be used as an independent predictor. Furthermore, upregulation of miR-590-5p inhibited cell migration and invasion in MGC-803 cells and HGC-27 cells. The serum expression level of exosomal miR-590-5p may be a biomarker, which is potentially useful and noninvasive for early detection and prediction of GC. In addition, miR-590-5p can play a role in eliminating carcinogens by actively regulating the malignant potential of gastric cancer. Frontiers Media S.A. 2021-02-12 /pmc/articles/PMC7928302/ /pubmed/33681295 http://dx.doi.org/10.3389/fmolb.2021.636566 Text en Copyright © 2021 Zheng, Xu, Hu, Lv, Xie, Huang, Zhang, Chen, Fu and Cheng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Zheng, Guo-Dian
Xu, Zhi-Yuan
Hu, Can
Lv, Hang
Xie, Hua-Xia
Huang, Ting
Zhang, Yan-Qiang
Chen, Gui-Ping
Fu, Yu-Fei
Cheng, Xiang-Dong
Exosomal miR-590-5p in Serum as a Biomarker for the Diagnosis and Prognosis of Gastric Cancer
title Exosomal miR-590-5p in Serum as a Biomarker for the Diagnosis and Prognosis of Gastric Cancer
title_full Exosomal miR-590-5p in Serum as a Biomarker for the Diagnosis and Prognosis of Gastric Cancer
title_fullStr Exosomal miR-590-5p in Serum as a Biomarker for the Diagnosis and Prognosis of Gastric Cancer
title_full_unstemmed Exosomal miR-590-5p in Serum as a Biomarker for the Diagnosis and Prognosis of Gastric Cancer
title_short Exosomal miR-590-5p in Serum as a Biomarker for the Diagnosis and Prognosis of Gastric Cancer
title_sort exosomal mir-590-5p in serum as a biomarker for the diagnosis and prognosis of gastric cancer
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928302/
https://www.ncbi.nlm.nih.gov/pubmed/33681295
http://dx.doi.org/10.3389/fmolb.2021.636566
work_keys_str_mv AT zhengguodian exosomalmir5905pinserumasabiomarkerforthediagnosisandprognosisofgastriccancer
AT xuzhiyuan exosomalmir5905pinserumasabiomarkerforthediagnosisandprognosisofgastriccancer
AT hucan exosomalmir5905pinserumasabiomarkerforthediagnosisandprognosisofgastriccancer
AT lvhang exosomalmir5905pinserumasabiomarkerforthediagnosisandprognosisofgastriccancer
AT xiehuaxia exosomalmir5905pinserumasabiomarkerforthediagnosisandprognosisofgastriccancer
AT huangting exosomalmir5905pinserumasabiomarkerforthediagnosisandprognosisofgastriccancer
AT zhangyanqiang exosomalmir5905pinserumasabiomarkerforthediagnosisandprognosisofgastriccancer
AT chenguiping exosomalmir5905pinserumasabiomarkerforthediagnosisandprognosisofgastriccancer
AT fuyufei exosomalmir5905pinserumasabiomarkerforthediagnosisandprognosisofgastriccancer
AT chengxiangdong exosomalmir5905pinserumasabiomarkerforthediagnosisandprognosisofgastriccancer