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Research on the Mechanism of Soybean Resistance to Phytophthora Infection Using Machine Learning Methods

Since the emergence of the Phytophthora sojae infection, economic losses of 10–20 billion U.S. dollars have been annually reported. Studies have revealed that P. sojae works by releasing effect factors such as small RNA in the process of infecting soybeans, but research on the interaction mechanism...

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Autores principales: Chi, Junxia, Song, Shizeng, Zhang, Hao, Liu, Yuanning, Zhao, Hengyi, Dong, Liyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928311/
https://www.ncbi.nlm.nih.gov/pubmed/33679898
http://dx.doi.org/10.3389/fgene.2021.634635
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author Chi, Junxia
Song, Shizeng
Zhang, Hao
Liu, Yuanning
Zhao, Hengyi
Dong, Liyan
author_facet Chi, Junxia
Song, Shizeng
Zhang, Hao
Liu, Yuanning
Zhao, Hengyi
Dong, Liyan
author_sort Chi, Junxia
collection PubMed
description Since the emergence of the Phytophthora sojae infection, economic losses of 10–20 billion U.S. dollars have been annually reported. Studies have revealed that P. sojae works by releasing effect factors such as small RNA in the process of infecting soybeans, but research on the interaction mechanism between plants and fungi at the small RNA level remains vague and unclear. For this reason, studying the resistance mechanism of the hosts after P. sojae invades soybeans has critical theoretical and practical significance for increasing soybean yield. The present article is premised on the high-throughput data published by the National Center of Biotechnology Information (NCBI). We selected 732 sRNA sequences through big data analysis whose expression level increased sharply after soybean was infected by P. sojae and 36 sRNA sequences with massive expression levels newly generated after infection. This article analyzes the resistance mechanism of soybean to P. sojae from two aspects of plant’s own passive stress and active resistance. These 768 sRNA sequences are targeted to soybean mRNA and P. sojae mRNA, and 2,979 and 1,683 targets are obtained, respectively. The PageRank algorithm was used to screen the core functional clusters, and 50 core nodes targeted to soybeans were obtained, which were analyzed for functional enrichment, and 12 KEGG_Pathway and 18 Go(BP) were obtained. The node targeted to P. sojae was subjected to functional enrichment analysis to obtain 11 KEGG_Pathway. The results show that there are multiple Go(BP) and KEGG_Pathway related to soybean growth and defense and reverse resistance of P. sojae. In addition, by comparing the small RNA prediction model of soybean resistance with Phytophthora pathogenicity constructed by the three machine learning methods of random forest, support vector machine, and XGBoost, about the accuracy, precision, recall rate, and F-measure, the results show that the three models have satisfied classification effect. Among the three models, XGBoost had an accuracy rate of 86.98% in the verification set.
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spelling pubmed-79283112021-03-04 Research on the Mechanism of Soybean Resistance to Phytophthora Infection Using Machine Learning Methods Chi, Junxia Song, Shizeng Zhang, Hao Liu, Yuanning Zhao, Hengyi Dong, Liyan Front Genet Genetics Since the emergence of the Phytophthora sojae infection, economic losses of 10–20 billion U.S. dollars have been annually reported. Studies have revealed that P. sojae works by releasing effect factors such as small RNA in the process of infecting soybeans, but research on the interaction mechanism between plants and fungi at the small RNA level remains vague and unclear. For this reason, studying the resistance mechanism of the hosts after P. sojae invades soybeans has critical theoretical and practical significance for increasing soybean yield. The present article is premised on the high-throughput data published by the National Center of Biotechnology Information (NCBI). We selected 732 sRNA sequences through big data analysis whose expression level increased sharply after soybean was infected by P. sojae and 36 sRNA sequences with massive expression levels newly generated after infection. This article analyzes the resistance mechanism of soybean to P. sojae from two aspects of plant’s own passive stress and active resistance. These 768 sRNA sequences are targeted to soybean mRNA and P. sojae mRNA, and 2,979 and 1,683 targets are obtained, respectively. The PageRank algorithm was used to screen the core functional clusters, and 50 core nodes targeted to soybeans were obtained, which were analyzed for functional enrichment, and 12 KEGG_Pathway and 18 Go(BP) were obtained. The node targeted to P. sojae was subjected to functional enrichment analysis to obtain 11 KEGG_Pathway. The results show that there are multiple Go(BP) and KEGG_Pathway related to soybean growth and defense and reverse resistance of P. sojae. In addition, by comparing the small RNA prediction model of soybean resistance with Phytophthora pathogenicity constructed by the three machine learning methods of random forest, support vector machine, and XGBoost, about the accuracy, precision, recall rate, and F-measure, the results show that the three models have satisfied classification effect. Among the three models, XGBoost had an accuracy rate of 86.98% in the verification set. Frontiers Media S.A. 2021-02-11 /pmc/articles/PMC7928311/ /pubmed/33679898 http://dx.doi.org/10.3389/fgene.2021.634635 Text en Copyright © 2021 Chi, Song, Zhang, Liu, Zhao and Dong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Chi, Junxia
Song, Shizeng
Zhang, Hao
Liu, Yuanning
Zhao, Hengyi
Dong, Liyan
Research on the Mechanism of Soybean Resistance to Phytophthora Infection Using Machine Learning Methods
title Research on the Mechanism of Soybean Resistance to Phytophthora Infection Using Machine Learning Methods
title_full Research on the Mechanism of Soybean Resistance to Phytophthora Infection Using Machine Learning Methods
title_fullStr Research on the Mechanism of Soybean Resistance to Phytophthora Infection Using Machine Learning Methods
title_full_unstemmed Research on the Mechanism of Soybean Resistance to Phytophthora Infection Using Machine Learning Methods
title_short Research on the Mechanism of Soybean Resistance to Phytophthora Infection Using Machine Learning Methods
title_sort research on the mechanism of soybean resistance to phytophthora infection using machine learning methods
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928311/
https://www.ncbi.nlm.nih.gov/pubmed/33679898
http://dx.doi.org/10.3389/fgene.2021.634635
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