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In vitro and in vivo Study on an Injectable Glycol Chitosan/Dibenzaldehyde-Terminated Polyethylene Glycol Hydrogel in Repairing Articular Cartilage Defects

The normal anatomical structure of articular cartilage determines its limited ability to regenerate and repair. Once damaged, it is difficult to repair it by itself. How to realize the regeneration and repair of articular cartilage has always been a big problem for clinicians and researchers. Here,...

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Autores principales: Yang, Jianhua, Jing, Xiaoguang, Wang, Zimin, Liu, Xuejian, Zhu, Xiaofeng, Lei, Tao, Li, Xu, Guo, Weimin, Rao, Haijun, Chen, Mingxue, Luan, Kai, Sui, Xiang, Wei, Yen, Liu, Shuyun, Guo, Quanyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928325/
https://www.ncbi.nlm.nih.gov/pubmed/33681156
http://dx.doi.org/10.3389/fbioe.2021.607709
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author Yang, Jianhua
Jing, Xiaoguang
Wang, Zimin
Liu, Xuejian
Zhu, Xiaofeng
Lei, Tao
Li, Xu
Guo, Weimin
Rao, Haijun
Chen, Mingxue
Luan, Kai
Sui, Xiang
Wei, Yen
Liu, Shuyun
Guo, Quanyi
author_facet Yang, Jianhua
Jing, Xiaoguang
Wang, Zimin
Liu, Xuejian
Zhu, Xiaofeng
Lei, Tao
Li, Xu
Guo, Weimin
Rao, Haijun
Chen, Mingxue
Luan, Kai
Sui, Xiang
Wei, Yen
Liu, Shuyun
Guo, Quanyi
author_sort Yang, Jianhua
collection PubMed
description The normal anatomical structure of articular cartilage determines its limited ability to regenerate and repair. Once damaged, it is difficult to repair it by itself. How to realize the regeneration and repair of articular cartilage has always been a big problem for clinicians and researchers. Here, we conducted a comprehensive analysis of the physical properties and cytocompatibility of hydrogels, and evaluated their feasibility as cell carriers for Adipose-derived mesenchymal stem cell (ADSC) transplantation. Concentration-matched hydrogels were co-cultured with ADSCs to confirm ADSC growth in the hydrogel and provide data supporting in vivo experiments, which comprised the hydrogel/ADSCs, pure-hydrogel, defect-placement, and positive-control groups. Rat models of articular cartilage defect in the knee joint region was generated, and each treatment was administered on the knee joint cartilage area for each group; in the positive-control group, the joint cavity was surgically opened, without inducing a cartilage defect. The reparative effect of injectable glycol chitosan/dibenzaldehyde-terminated polyethylene glycol (GCS/DF-PEG) hydrogel on injured articular cartilage was evaluated by measuring gross scores and histological score of knee joint articular-cartilage injury in rats after 8 weeks. The 1.5% GCS/2% DF-PEG hydrogels degraded quickly in vitro. Then, We perform in vivo and in vitro experiments to evaluate the feasibility of this material for cartilage repair in vivo and in vitro.
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spelling pubmed-79283252021-03-04 In vitro and in vivo Study on an Injectable Glycol Chitosan/Dibenzaldehyde-Terminated Polyethylene Glycol Hydrogel in Repairing Articular Cartilage Defects Yang, Jianhua Jing, Xiaoguang Wang, Zimin Liu, Xuejian Zhu, Xiaofeng Lei, Tao Li, Xu Guo, Weimin Rao, Haijun Chen, Mingxue Luan, Kai Sui, Xiang Wei, Yen Liu, Shuyun Guo, Quanyi Front Bioeng Biotechnol Bioengineering and Biotechnology The normal anatomical structure of articular cartilage determines its limited ability to regenerate and repair. Once damaged, it is difficult to repair it by itself. How to realize the regeneration and repair of articular cartilage has always been a big problem for clinicians and researchers. Here, we conducted a comprehensive analysis of the physical properties and cytocompatibility of hydrogels, and evaluated their feasibility as cell carriers for Adipose-derived mesenchymal stem cell (ADSC) transplantation. Concentration-matched hydrogels were co-cultured with ADSCs to confirm ADSC growth in the hydrogel and provide data supporting in vivo experiments, which comprised the hydrogel/ADSCs, pure-hydrogel, defect-placement, and positive-control groups. Rat models of articular cartilage defect in the knee joint region was generated, and each treatment was administered on the knee joint cartilage area for each group; in the positive-control group, the joint cavity was surgically opened, without inducing a cartilage defect. The reparative effect of injectable glycol chitosan/dibenzaldehyde-terminated polyethylene glycol (GCS/DF-PEG) hydrogel on injured articular cartilage was evaluated by measuring gross scores and histological score of knee joint articular-cartilage injury in rats after 8 weeks. The 1.5% GCS/2% DF-PEG hydrogels degraded quickly in vitro. Then, We perform in vivo and in vitro experiments to evaluate the feasibility of this material for cartilage repair in vivo and in vitro. Frontiers Media S.A. 2021-02-16 /pmc/articles/PMC7928325/ /pubmed/33681156 http://dx.doi.org/10.3389/fbioe.2021.607709 Text en Copyright © 2021 Yang, Jing, Wang, Liu, Zhu, Lei, Li, Guo, Rao, Chen, Luan, Sui, Wei, Liu and Guo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Yang, Jianhua
Jing, Xiaoguang
Wang, Zimin
Liu, Xuejian
Zhu, Xiaofeng
Lei, Tao
Li, Xu
Guo, Weimin
Rao, Haijun
Chen, Mingxue
Luan, Kai
Sui, Xiang
Wei, Yen
Liu, Shuyun
Guo, Quanyi
In vitro and in vivo Study on an Injectable Glycol Chitosan/Dibenzaldehyde-Terminated Polyethylene Glycol Hydrogel in Repairing Articular Cartilage Defects
title In vitro and in vivo Study on an Injectable Glycol Chitosan/Dibenzaldehyde-Terminated Polyethylene Glycol Hydrogel in Repairing Articular Cartilage Defects
title_full In vitro and in vivo Study on an Injectable Glycol Chitosan/Dibenzaldehyde-Terminated Polyethylene Glycol Hydrogel in Repairing Articular Cartilage Defects
title_fullStr In vitro and in vivo Study on an Injectable Glycol Chitosan/Dibenzaldehyde-Terminated Polyethylene Glycol Hydrogel in Repairing Articular Cartilage Defects
title_full_unstemmed In vitro and in vivo Study on an Injectable Glycol Chitosan/Dibenzaldehyde-Terminated Polyethylene Glycol Hydrogel in Repairing Articular Cartilage Defects
title_short In vitro and in vivo Study on an Injectable Glycol Chitosan/Dibenzaldehyde-Terminated Polyethylene Glycol Hydrogel in Repairing Articular Cartilage Defects
title_sort in vitro and in vivo study on an injectable glycol chitosan/dibenzaldehyde-terminated polyethylene glycol hydrogel in repairing articular cartilage defects
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928325/
https://www.ncbi.nlm.nih.gov/pubmed/33681156
http://dx.doi.org/10.3389/fbioe.2021.607709
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