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Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb)
Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, in chromatin remodeling, as well as the expression of non-coding RNAs (ncRNAs). However, very little is known about the interactions between micro...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928366/ https://www.ncbi.nlm.nih.gov/pubmed/33679885 http://dx.doi.org/10.3389/fgene.2021.620744 |
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author | de Araújo, Marília Ladeira Gomes, Bruno Costa Devóz, Paula Pícoli Duarte, Nathália de Assis Aguilar Ribeiro, Diego Luis de Araújo, Adriana Ladeira Batista, Bruno Lemos Antunes, Lusânia Maria Greggi Barbosa, Fernando Rodrigues, António Sebastião Rueff, José Barcelos, Gustavo Rafael Mazzaron |
author_facet | de Araújo, Marília Ladeira Gomes, Bruno Costa Devóz, Paula Pícoli Duarte, Nathália de Assis Aguilar Ribeiro, Diego Luis de Araújo, Adriana Ladeira Batista, Bruno Lemos Antunes, Lusânia Maria Greggi Barbosa, Fernando Rodrigues, António Sebastião Rueff, José Barcelos, Gustavo Rafael Mazzaron |
author_sort | de Araújo, Marília Ladeira |
collection | PubMed |
description | Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, in chromatin remodeling, as well as the expression of non-coding RNAs (ncRNAs). However, very little is known about the interactions between microRNAs (miRNAs) expression and DNA methylation status in individuals exposed to the metal. The aim of the present study was to investigate the impact of hsa-miR-148a expression on DNA methylation status, in 85 workers exposed to Pb. Blood and plasma lead levels (BLL and PLL, respectively) were determined by ICP-MS; expression of the miRNA-148a was quantified by RT-qPCR (TaqMan assay) and assessment of the global DNA methylation profile (by measurement of 5-methylcytosine; % 5-mC) was performed by ELISA. An inverse association was seen between miR-148a and % 5-mC DNA, as a function of BLL and PLL (β = −3.7; p = 0.071 and β = −4.1; p = 0.049, respectively) adjusted for age, BMI, smoking, and alcohol consumption. Taken together, our study provides further evidence concerning the interactions between DNA methylation profile and miR-148a, in individuals exposed to Pb. |
format | Online Article Text |
id | pubmed-7928366 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79283662021-03-04 Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) de Araújo, Marília Ladeira Gomes, Bruno Costa Devóz, Paula Pícoli Duarte, Nathália de Assis Aguilar Ribeiro, Diego Luis de Araújo, Adriana Ladeira Batista, Bruno Lemos Antunes, Lusânia Maria Greggi Barbosa, Fernando Rodrigues, António Sebastião Rueff, José Barcelos, Gustavo Rafael Mazzaron Front Genet Genetics Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, in chromatin remodeling, as well as the expression of non-coding RNAs (ncRNAs). However, very little is known about the interactions between microRNAs (miRNAs) expression and DNA methylation status in individuals exposed to the metal. The aim of the present study was to investigate the impact of hsa-miR-148a expression on DNA methylation status, in 85 workers exposed to Pb. Blood and plasma lead levels (BLL and PLL, respectively) were determined by ICP-MS; expression of the miRNA-148a was quantified by RT-qPCR (TaqMan assay) and assessment of the global DNA methylation profile (by measurement of 5-methylcytosine; % 5-mC) was performed by ELISA. An inverse association was seen between miR-148a and % 5-mC DNA, as a function of BLL and PLL (β = −3.7; p = 0.071 and β = −4.1; p = 0.049, respectively) adjusted for age, BMI, smoking, and alcohol consumption. Taken together, our study provides further evidence concerning the interactions between DNA methylation profile and miR-148a, in individuals exposed to Pb. Frontiers Media S.A. 2021-02-17 /pmc/articles/PMC7928366/ /pubmed/33679885 http://dx.doi.org/10.3389/fgene.2021.620744 Text en Copyright © 2021 Araújo, Gomes, Devóz, Duarte, Ribeiro, Araújo, Batista, Antunes, Barbosa, Rodrigues, Rueff and Barcelos. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics de Araújo, Marília Ladeira Gomes, Bruno Costa Devóz, Paula Pícoli Duarte, Nathália de Assis Aguilar Ribeiro, Diego Luis de Araújo, Adriana Ladeira Batista, Bruno Lemos Antunes, Lusânia Maria Greggi Barbosa, Fernando Rodrigues, António Sebastião Rueff, José Barcelos, Gustavo Rafael Mazzaron Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) |
title | Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) |
title_full | Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) |
title_fullStr | Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) |
title_full_unstemmed | Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) |
title_short | Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) |
title_sort | association between mir-148a and dna methylation profile in individuals exposed to lead (pb) |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928366/ https://www.ncbi.nlm.nih.gov/pubmed/33679885 http://dx.doi.org/10.3389/fgene.2021.620744 |
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