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Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb)

Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, in chromatin remodeling, as well as the expression of non-coding RNAs (ncRNAs). However, very little is known about the interactions between micro...

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Autores principales: de Araújo, Marília Ladeira, Gomes, Bruno Costa, Devóz, Paula Pícoli, Duarte, Nathália de Assis Aguilar, Ribeiro, Diego Luis, de Araújo, Adriana Ladeira, Batista, Bruno Lemos, Antunes, Lusânia Maria Greggi, Barbosa, Fernando, Rodrigues, António Sebastião, Rueff, José, Barcelos, Gustavo Rafael Mazzaron
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928366/
https://www.ncbi.nlm.nih.gov/pubmed/33679885
http://dx.doi.org/10.3389/fgene.2021.620744
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author de Araújo, Marília Ladeira
Gomes, Bruno Costa
Devóz, Paula Pícoli
Duarte, Nathália de Assis Aguilar
Ribeiro, Diego Luis
de Araújo, Adriana Ladeira
Batista, Bruno Lemos
Antunes, Lusânia Maria Greggi
Barbosa, Fernando
Rodrigues, António Sebastião
Rueff, José
Barcelos, Gustavo Rafael Mazzaron
author_facet de Araújo, Marília Ladeira
Gomes, Bruno Costa
Devóz, Paula Pícoli
Duarte, Nathália de Assis Aguilar
Ribeiro, Diego Luis
de Araújo, Adriana Ladeira
Batista, Bruno Lemos
Antunes, Lusânia Maria Greggi
Barbosa, Fernando
Rodrigues, António Sebastião
Rueff, José
Barcelos, Gustavo Rafael Mazzaron
author_sort de Araújo, Marília Ladeira
collection PubMed
description Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, in chromatin remodeling, as well as the expression of non-coding RNAs (ncRNAs). However, very little is known about the interactions between microRNAs (miRNAs) expression and DNA methylation status in individuals exposed to the metal. The aim of the present study was to investigate the impact of hsa-miR-148a expression on DNA methylation status, in 85 workers exposed to Pb. Blood and plasma lead levels (BLL and PLL, respectively) were determined by ICP-MS; expression of the miRNA-148a was quantified by RT-qPCR (TaqMan assay) and assessment of the global DNA methylation profile (by measurement of 5-methylcytosine; % 5-mC) was performed by ELISA. An inverse association was seen between miR-148a and % 5-mC DNA, as a function of BLL and PLL (β = −3.7; p = 0.071 and β = −4.1; p = 0.049, respectively) adjusted for age, BMI, smoking, and alcohol consumption. Taken together, our study provides further evidence concerning the interactions between DNA methylation profile and miR-148a, in individuals exposed to Pb.
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spelling pubmed-79283662021-03-04 Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) de Araújo, Marília Ladeira Gomes, Bruno Costa Devóz, Paula Pícoli Duarte, Nathália de Assis Aguilar Ribeiro, Diego Luis de Araújo, Adriana Ladeira Batista, Bruno Lemos Antunes, Lusânia Maria Greggi Barbosa, Fernando Rodrigues, António Sebastião Rueff, José Barcelos, Gustavo Rafael Mazzaron Front Genet Genetics Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, in chromatin remodeling, as well as the expression of non-coding RNAs (ncRNAs). However, very little is known about the interactions between microRNAs (miRNAs) expression and DNA methylation status in individuals exposed to the metal. The aim of the present study was to investigate the impact of hsa-miR-148a expression on DNA methylation status, in 85 workers exposed to Pb. Blood and plasma lead levels (BLL and PLL, respectively) were determined by ICP-MS; expression of the miRNA-148a was quantified by RT-qPCR (TaqMan assay) and assessment of the global DNA methylation profile (by measurement of 5-methylcytosine; % 5-mC) was performed by ELISA. An inverse association was seen between miR-148a and % 5-mC DNA, as a function of BLL and PLL (β = −3.7; p = 0.071 and β = −4.1; p = 0.049, respectively) adjusted for age, BMI, smoking, and alcohol consumption. Taken together, our study provides further evidence concerning the interactions between DNA methylation profile and miR-148a, in individuals exposed to Pb. Frontiers Media S.A. 2021-02-17 /pmc/articles/PMC7928366/ /pubmed/33679885 http://dx.doi.org/10.3389/fgene.2021.620744 Text en Copyright © 2021 Araújo, Gomes, Devóz, Duarte, Ribeiro, Araújo, Batista, Antunes, Barbosa, Rodrigues, Rueff and Barcelos. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
de Araújo, Marília Ladeira
Gomes, Bruno Costa
Devóz, Paula Pícoli
Duarte, Nathália de Assis Aguilar
Ribeiro, Diego Luis
de Araújo, Adriana Ladeira
Batista, Bruno Lemos
Antunes, Lusânia Maria Greggi
Barbosa, Fernando
Rodrigues, António Sebastião
Rueff, José
Barcelos, Gustavo Rafael Mazzaron
Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb)
title Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb)
title_full Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb)
title_fullStr Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb)
title_full_unstemmed Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb)
title_short Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb)
title_sort association between mir-148a and dna methylation profile in individuals exposed to lead (pb)
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928366/
https://www.ncbi.nlm.nih.gov/pubmed/33679885
http://dx.doi.org/10.3389/fgene.2021.620744
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