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Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept

Isolation of the pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis from foods typically rely on slow (10–21 day) “cold enrichment” protocols before confirmed results are obtained. We describe an approach that yields results in 39 h that combines an alternative enrichment method with...

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Autores principales: On, Stephen L. W., Zhang, Yuwei, Gehring, Andrew, Patsekin, Valery, Chelikani, Venkata, Flint, Steve, Wang, Haoran, Billington, Craig, Fletcher, Graham C., Lindsay, James, Robinson, J. Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928378/
https://www.ncbi.nlm.nih.gov/pubmed/33679677
http://dx.doi.org/10.3389/fmicb.2021.641801
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author On, Stephen L. W.
Zhang, Yuwei
Gehring, Andrew
Patsekin, Valery
Chelikani, Venkata
Flint, Steve
Wang, Haoran
Billington, Craig
Fletcher, Graham C.
Lindsay, James
Robinson, J. Paul
author_facet On, Stephen L. W.
Zhang, Yuwei
Gehring, Andrew
Patsekin, Valery
Chelikani, Venkata
Flint, Steve
Wang, Haoran
Billington, Craig
Fletcher, Graham C.
Lindsay, James
Robinson, J. Paul
author_sort On, Stephen L. W.
collection PubMed
description Isolation of the pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis from foods typically rely on slow (10–21 day) “cold enrichment” protocols before confirmed results are obtained. We describe an approach that yields results in 39 h that combines an alternative enrichment method with culture on a non-selective medium, and subsequent identification of suspect colonies using elastic light scatter (ELS) analysis. A prototype database of ELS profiles from five Yersinia species and six other bacterial genera found in pork mince was established, and used to compare similar profiles of colonies obtained from enrichment cultures from pork mince samples seeded with representative strains of Y. enterocolitica and Y. pseudotuberculosis. The presumptive identification by ELS using computerised or visual analyses of 83/90 colonies in these experiments as the target species was confirmed by partial 16S rDNA sequencing. In addition to seeded cultures, our method recovered two naturally occurring Yersinia strains. Our results indicate that modified enrichment combined with ELS is a promising new approach for expedited detection of foodborne pathogenic yersiniae.
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spelling pubmed-79283782021-03-04 Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept On, Stephen L. W. Zhang, Yuwei Gehring, Andrew Patsekin, Valery Chelikani, Venkata Flint, Steve Wang, Haoran Billington, Craig Fletcher, Graham C. Lindsay, James Robinson, J. Paul Front Microbiol Microbiology Isolation of the pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis from foods typically rely on slow (10–21 day) “cold enrichment” protocols before confirmed results are obtained. We describe an approach that yields results in 39 h that combines an alternative enrichment method with culture on a non-selective medium, and subsequent identification of suspect colonies using elastic light scatter (ELS) analysis. A prototype database of ELS profiles from five Yersinia species and six other bacterial genera found in pork mince was established, and used to compare similar profiles of colonies obtained from enrichment cultures from pork mince samples seeded with representative strains of Y. enterocolitica and Y. pseudotuberculosis. The presumptive identification by ELS using computerised or visual analyses of 83/90 colonies in these experiments as the target species was confirmed by partial 16S rDNA sequencing. In addition to seeded cultures, our method recovered two naturally occurring Yersinia strains. Our results indicate that modified enrichment combined with ELS is a promising new approach for expedited detection of foodborne pathogenic yersiniae. Frontiers Media S.A. 2021-02-17 /pmc/articles/PMC7928378/ /pubmed/33679677 http://dx.doi.org/10.3389/fmicb.2021.641801 Text en Copyright © 2021 On, Zhang, Gehring, Patsekin, Chelikani, Flint, Wang, Billington, Fletcher, Lindsay and Robinson. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
On, Stephen L. W.
Zhang, Yuwei
Gehring, Andrew
Patsekin, Valery
Chelikani, Venkata
Flint, Steve
Wang, Haoran
Billington, Craig
Fletcher, Graham C.
Lindsay, James
Robinson, J. Paul
Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept
title Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept
title_full Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept
title_fullStr Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept
title_full_unstemmed Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept
title_short Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept
title_sort elastic light scatter pattern analysis for the expedited detection of yersinia species in pork mince: proof of concept
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928378/
https://www.ncbi.nlm.nih.gov/pubmed/33679677
http://dx.doi.org/10.3389/fmicb.2021.641801
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