Cargando…
Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept
Isolation of the pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis from foods typically rely on slow (10–21 day) “cold enrichment” protocols before confirmed results are obtained. We describe an approach that yields results in 39 h that combines an alternative enrichment method with...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928378/ https://www.ncbi.nlm.nih.gov/pubmed/33679677 http://dx.doi.org/10.3389/fmicb.2021.641801 |
_version_ | 1783659843746267136 |
---|---|
author | On, Stephen L. W. Zhang, Yuwei Gehring, Andrew Patsekin, Valery Chelikani, Venkata Flint, Steve Wang, Haoran Billington, Craig Fletcher, Graham C. Lindsay, James Robinson, J. Paul |
author_facet | On, Stephen L. W. Zhang, Yuwei Gehring, Andrew Patsekin, Valery Chelikani, Venkata Flint, Steve Wang, Haoran Billington, Craig Fletcher, Graham C. Lindsay, James Robinson, J. Paul |
author_sort | On, Stephen L. W. |
collection | PubMed |
description | Isolation of the pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis from foods typically rely on slow (10–21 day) “cold enrichment” protocols before confirmed results are obtained. We describe an approach that yields results in 39 h that combines an alternative enrichment method with culture on a non-selective medium, and subsequent identification of suspect colonies using elastic light scatter (ELS) analysis. A prototype database of ELS profiles from five Yersinia species and six other bacterial genera found in pork mince was established, and used to compare similar profiles of colonies obtained from enrichment cultures from pork mince samples seeded with representative strains of Y. enterocolitica and Y. pseudotuberculosis. The presumptive identification by ELS using computerised or visual analyses of 83/90 colonies in these experiments as the target species was confirmed by partial 16S rDNA sequencing. In addition to seeded cultures, our method recovered two naturally occurring Yersinia strains. Our results indicate that modified enrichment combined with ELS is a promising new approach for expedited detection of foodborne pathogenic yersiniae. |
format | Online Article Text |
id | pubmed-7928378 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79283782021-03-04 Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept On, Stephen L. W. Zhang, Yuwei Gehring, Andrew Patsekin, Valery Chelikani, Venkata Flint, Steve Wang, Haoran Billington, Craig Fletcher, Graham C. Lindsay, James Robinson, J. Paul Front Microbiol Microbiology Isolation of the pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis from foods typically rely on slow (10–21 day) “cold enrichment” protocols before confirmed results are obtained. We describe an approach that yields results in 39 h that combines an alternative enrichment method with culture on a non-selective medium, and subsequent identification of suspect colonies using elastic light scatter (ELS) analysis. A prototype database of ELS profiles from five Yersinia species and six other bacterial genera found in pork mince was established, and used to compare similar profiles of colonies obtained from enrichment cultures from pork mince samples seeded with representative strains of Y. enterocolitica and Y. pseudotuberculosis. The presumptive identification by ELS using computerised or visual analyses of 83/90 colonies in these experiments as the target species was confirmed by partial 16S rDNA sequencing. In addition to seeded cultures, our method recovered two naturally occurring Yersinia strains. Our results indicate that modified enrichment combined with ELS is a promising new approach for expedited detection of foodborne pathogenic yersiniae. Frontiers Media S.A. 2021-02-17 /pmc/articles/PMC7928378/ /pubmed/33679677 http://dx.doi.org/10.3389/fmicb.2021.641801 Text en Copyright © 2021 On, Zhang, Gehring, Patsekin, Chelikani, Flint, Wang, Billington, Fletcher, Lindsay and Robinson. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology On, Stephen L. W. Zhang, Yuwei Gehring, Andrew Patsekin, Valery Chelikani, Venkata Flint, Steve Wang, Haoran Billington, Craig Fletcher, Graham C. Lindsay, James Robinson, J. Paul Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept |
title | Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept |
title_full | Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept |
title_fullStr | Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept |
title_full_unstemmed | Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept |
title_short | Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept |
title_sort | elastic light scatter pattern analysis for the expedited detection of yersinia species in pork mince: proof of concept |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928378/ https://www.ncbi.nlm.nih.gov/pubmed/33679677 http://dx.doi.org/10.3389/fmicb.2021.641801 |
work_keys_str_mv | AT onstephenlw elasticlightscatterpatternanalysisfortheexpediteddetectionofyersiniaspeciesinporkminceproofofconcept AT zhangyuwei elasticlightscatterpatternanalysisfortheexpediteddetectionofyersiniaspeciesinporkminceproofofconcept AT gehringandrew elasticlightscatterpatternanalysisfortheexpediteddetectionofyersiniaspeciesinporkminceproofofconcept AT patsekinvalery elasticlightscatterpatternanalysisfortheexpediteddetectionofyersiniaspeciesinporkminceproofofconcept AT chelikanivenkata elasticlightscatterpatternanalysisfortheexpediteddetectionofyersiniaspeciesinporkminceproofofconcept AT flintsteve elasticlightscatterpatternanalysisfortheexpediteddetectionofyersiniaspeciesinporkminceproofofconcept AT wanghaoran elasticlightscatterpatternanalysisfortheexpediteddetectionofyersiniaspeciesinporkminceproofofconcept AT billingtoncraig elasticlightscatterpatternanalysisfortheexpediteddetectionofyersiniaspeciesinporkminceproofofconcept AT fletchergrahamc elasticlightscatterpatternanalysisfortheexpediteddetectionofyersiniaspeciesinporkminceproofofconcept AT lindsayjames elasticlightscatterpatternanalysisfortheexpediteddetectionofyersiniaspeciesinporkminceproofofconcept AT robinsonjpaul elasticlightscatterpatternanalysisfortheexpediteddetectionofyersiniaspeciesinporkminceproofofconcept |