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Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage
Tellurium oxyanion, tellurite (TeO(3)(–2)), is a highly toxic compound for many organisms. Its presence in the environment has increased over the past years due to industrial manufacturing processes and has been associated with adverse effects on human health. Although tellurite induces the phosphor...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928414/ https://www.ncbi.nlm.nih.gov/pubmed/33681200 http://dx.doi.org/10.3389/fcell.2021.622057 |
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author | Gaete-Argel, Aracelly Velásquez, Felipe Márquez, Chantal L. Rojas-Araya, Barbara Bueno-Nieto, Constanza Marín-Rojas, Josefina Cuevas-Zúñiga, Miguel Soto-Rifo, Ricardo Valiente-Echeverría, Fernando |
author_facet | Gaete-Argel, Aracelly Velásquez, Felipe Márquez, Chantal L. Rojas-Araya, Barbara Bueno-Nieto, Constanza Marín-Rojas, Josefina Cuevas-Zúñiga, Miguel Soto-Rifo, Ricardo Valiente-Echeverría, Fernando |
author_sort | Gaete-Argel, Aracelly |
collection | PubMed |
description | Tellurium oxyanion, tellurite (TeO(3)(–2)), is a highly toxic compound for many organisms. Its presence in the environment has increased over the past years due to industrial manufacturing processes and has been associated with adverse effects on human health. Although tellurite induces the phosphorylation of eIF2α, DNA damage and oxidative stress, the molecular mechanisms related to the cellular responses to tellurite-induced stress are poorly understood. In this work, we evaluated the ability of tellurite to induce phosphorylation of eIF2α, stress granules (SGs) assembly and their relationship with DNA damage in U2OS cells. We demonstrate that tellurite promotes the assembly of bona fide cytoplasmic SGs. Unexpectedly, tellurite also induces the assembly of nuclear SGs. Interestingly, we observed that the presence of tellurite-induced nuclear SGs correlates with γH2AX foci. However, although H(2)O(2) also induce DNA damage, no nuclear SGs were observed. Our data show that tellurite promotes the assembly of cytoplasmic and nuclear SGs in response to oxidative stress and DNA damage, revealing a new aspect of cellular stress response mediated by the assembly of nuclear stress granules. |
format | Online Article Text |
id | pubmed-7928414 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79284142021-03-04 Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage Gaete-Argel, Aracelly Velásquez, Felipe Márquez, Chantal L. Rojas-Araya, Barbara Bueno-Nieto, Constanza Marín-Rojas, Josefina Cuevas-Zúñiga, Miguel Soto-Rifo, Ricardo Valiente-Echeverría, Fernando Front Cell Dev Biol Cell and Developmental Biology Tellurium oxyanion, tellurite (TeO(3)(–2)), is a highly toxic compound for many organisms. Its presence in the environment has increased over the past years due to industrial manufacturing processes and has been associated with adverse effects on human health. Although tellurite induces the phosphorylation of eIF2α, DNA damage and oxidative stress, the molecular mechanisms related to the cellular responses to tellurite-induced stress are poorly understood. In this work, we evaluated the ability of tellurite to induce phosphorylation of eIF2α, stress granules (SGs) assembly and their relationship with DNA damage in U2OS cells. We demonstrate that tellurite promotes the assembly of bona fide cytoplasmic SGs. Unexpectedly, tellurite also induces the assembly of nuclear SGs. Interestingly, we observed that the presence of tellurite-induced nuclear SGs correlates with γH2AX foci. However, although H(2)O(2) also induce DNA damage, no nuclear SGs were observed. Our data show that tellurite promotes the assembly of cytoplasmic and nuclear SGs in response to oxidative stress and DNA damage, revealing a new aspect of cellular stress response mediated by the assembly of nuclear stress granules. Frontiers Media S.A. 2021-02-11 /pmc/articles/PMC7928414/ /pubmed/33681200 http://dx.doi.org/10.3389/fcell.2021.622057 Text en Copyright © 2021 Gaete-Argel, Velásquez, Márquez, Rojas-Araya, Bueno-Nieto, Marín-Rojas, Cuevas-Zúñiga, Soto-Rifo and Valiente-Echeverría. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Gaete-Argel, Aracelly Velásquez, Felipe Márquez, Chantal L. Rojas-Araya, Barbara Bueno-Nieto, Constanza Marín-Rojas, Josefina Cuevas-Zúñiga, Miguel Soto-Rifo, Ricardo Valiente-Echeverría, Fernando Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage |
title | Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage |
title_full | Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage |
title_fullStr | Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage |
title_full_unstemmed | Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage |
title_short | Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage |
title_sort | tellurite promotes stress granules and nuclear sg-like assembly in response to oxidative stress and dna damage |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928414/ https://www.ncbi.nlm.nih.gov/pubmed/33681200 http://dx.doi.org/10.3389/fcell.2021.622057 |
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