Cargando…

Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage

Tellurium oxyanion, tellurite (TeO(3)(–2)), is a highly toxic compound for many organisms. Its presence in the environment has increased over the past years due to industrial manufacturing processes and has been associated with adverse effects on human health. Although tellurite induces the phosphor...

Descripción completa

Detalles Bibliográficos
Autores principales: Gaete-Argel, Aracelly, Velásquez, Felipe, Márquez, Chantal L., Rojas-Araya, Barbara, Bueno-Nieto, Constanza, Marín-Rojas, Josefina, Cuevas-Zúñiga, Miguel, Soto-Rifo, Ricardo, Valiente-Echeverría, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928414/
https://www.ncbi.nlm.nih.gov/pubmed/33681200
http://dx.doi.org/10.3389/fcell.2021.622057
_version_ 1783659852251267072
author Gaete-Argel, Aracelly
Velásquez, Felipe
Márquez, Chantal L.
Rojas-Araya, Barbara
Bueno-Nieto, Constanza
Marín-Rojas, Josefina
Cuevas-Zúñiga, Miguel
Soto-Rifo, Ricardo
Valiente-Echeverría, Fernando
author_facet Gaete-Argel, Aracelly
Velásquez, Felipe
Márquez, Chantal L.
Rojas-Araya, Barbara
Bueno-Nieto, Constanza
Marín-Rojas, Josefina
Cuevas-Zúñiga, Miguel
Soto-Rifo, Ricardo
Valiente-Echeverría, Fernando
author_sort Gaete-Argel, Aracelly
collection PubMed
description Tellurium oxyanion, tellurite (TeO(3)(–2)), is a highly toxic compound for many organisms. Its presence in the environment has increased over the past years due to industrial manufacturing processes and has been associated with adverse effects on human health. Although tellurite induces the phosphorylation of eIF2α, DNA damage and oxidative stress, the molecular mechanisms related to the cellular responses to tellurite-induced stress are poorly understood. In this work, we evaluated the ability of tellurite to induce phosphorylation of eIF2α, stress granules (SGs) assembly and their relationship with DNA damage in U2OS cells. We demonstrate that tellurite promotes the assembly of bona fide cytoplasmic SGs. Unexpectedly, tellurite also induces the assembly of nuclear SGs. Interestingly, we observed that the presence of tellurite-induced nuclear SGs correlates with γH2AX foci. However, although H(2)O(2) also induce DNA damage, no nuclear SGs were observed. Our data show that tellurite promotes the assembly of cytoplasmic and nuclear SGs in response to oxidative stress and DNA damage, revealing a new aspect of cellular stress response mediated by the assembly of nuclear stress granules.
format Online
Article
Text
id pubmed-7928414
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-79284142021-03-04 Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage Gaete-Argel, Aracelly Velásquez, Felipe Márquez, Chantal L. Rojas-Araya, Barbara Bueno-Nieto, Constanza Marín-Rojas, Josefina Cuevas-Zúñiga, Miguel Soto-Rifo, Ricardo Valiente-Echeverría, Fernando Front Cell Dev Biol Cell and Developmental Biology Tellurium oxyanion, tellurite (TeO(3)(–2)), is a highly toxic compound for many organisms. Its presence in the environment has increased over the past years due to industrial manufacturing processes and has been associated with adverse effects on human health. Although tellurite induces the phosphorylation of eIF2α, DNA damage and oxidative stress, the molecular mechanisms related to the cellular responses to tellurite-induced stress are poorly understood. In this work, we evaluated the ability of tellurite to induce phosphorylation of eIF2α, stress granules (SGs) assembly and their relationship with DNA damage in U2OS cells. We demonstrate that tellurite promotes the assembly of bona fide cytoplasmic SGs. Unexpectedly, tellurite also induces the assembly of nuclear SGs. Interestingly, we observed that the presence of tellurite-induced nuclear SGs correlates with γH2AX foci. However, although H(2)O(2) also induce DNA damage, no nuclear SGs were observed. Our data show that tellurite promotes the assembly of cytoplasmic and nuclear SGs in response to oxidative stress and DNA damage, revealing a new aspect of cellular stress response mediated by the assembly of nuclear stress granules. Frontiers Media S.A. 2021-02-11 /pmc/articles/PMC7928414/ /pubmed/33681200 http://dx.doi.org/10.3389/fcell.2021.622057 Text en Copyright © 2021 Gaete-Argel, Velásquez, Márquez, Rojas-Araya, Bueno-Nieto, Marín-Rojas, Cuevas-Zúñiga, Soto-Rifo and Valiente-Echeverría. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Gaete-Argel, Aracelly
Velásquez, Felipe
Márquez, Chantal L.
Rojas-Araya, Barbara
Bueno-Nieto, Constanza
Marín-Rojas, Josefina
Cuevas-Zúñiga, Miguel
Soto-Rifo, Ricardo
Valiente-Echeverría, Fernando
Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage
title Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage
title_full Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage
title_fullStr Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage
title_full_unstemmed Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage
title_short Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage
title_sort tellurite promotes stress granules and nuclear sg-like assembly in response to oxidative stress and dna damage
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928414/
https://www.ncbi.nlm.nih.gov/pubmed/33681200
http://dx.doi.org/10.3389/fcell.2021.622057
work_keys_str_mv AT gaeteargelaracelly telluritepromotesstressgranulesandnuclearsglikeassemblyinresponsetooxidativestressanddnadamage
AT velasquezfelipe telluritepromotesstressgranulesandnuclearsglikeassemblyinresponsetooxidativestressanddnadamage
AT marquezchantall telluritepromotesstressgranulesandnuclearsglikeassemblyinresponsetooxidativestressanddnadamage
AT rojasarayabarbara telluritepromotesstressgranulesandnuclearsglikeassemblyinresponsetooxidativestressanddnadamage
AT buenonietoconstanza telluritepromotesstressgranulesandnuclearsglikeassemblyinresponsetooxidativestressanddnadamage
AT marinrojasjosefina telluritepromotesstressgranulesandnuclearsglikeassemblyinresponsetooxidativestressanddnadamage
AT cuevaszunigamiguel telluritepromotesstressgranulesandnuclearsglikeassemblyinresponsetooxidativestressanddnadamage
AT sotoriforicardo telluritepromotesstressgranulesandnuclearsglikeassemblyinresponsetooxidativestressanddnadamage
AT valienteecheverriafernando telluritepromotesstressgranulesandnuclearsglikeassemblyinresponsetooxidativestressanddnadamage