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Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples

Nontuberculous mycobacteria (NTM) are waterborne pathogens commonly found in building water systems where they are a primary concern to vulnerable patient populations and can cause severe disease. The recovery of NTM from environmental samples can be a laborious undertaking and current pre-treatment...

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Autores principales: Alexander, Kimberly J., Furlong, Jennifer L., Baron, Julianne L., Rihs, John D., Stephenson, Dominic, Perry, John D., Stout, Janet E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928522/
https://www.ncbi.nlm.nih.gov/pubmed/33657154
http://dx.doi.org/10.1371/journal.pone.0247166
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author Alexander, Kimberly J.
Furlong, Jennifer L.
Baron, Julianne L.
Rihs, John D.
Stephenson, Dominic
Perry, John D.
Stout, Janet E.
author_facet Alexander, Kimberly J.
Furlong, Jennifer L.
Baron, Julianne L.
Rihs, John D.
Stephenson, Dominic
Perry, John D.
Stout, Janet E.
author_sort Alexander, Kimberly J.
collection PubMed
description Nontuberculous mycobacteria (NTM) are waterborne pathogens commonly found in building water systems where they are a primary concern to vulnerable patient populations and can cause severe disease. The recovery of NTM from environmental samples can be a laborious undertaking and current pre-treatment methods and selective media lack sensitivity. We explored the use of the highly selective Rapidly Growing Mycobacteria (RGM) medium for culturing NTM from environmental water samples compared to existing methods. In total, 223 environmental water samples, including potable and non-potable water, were cultured for NTM using three culture media. In addition to direct culture on RGM medium, each sample was cultured on Middlebrook 7H10 medium and Mitchison 7H11 medium after pre-treatment with 0.2M KCl-HCl. Additionally, 33 distinct species of NTM were inoculated onto RGM medium and 7H10 medium in parallel to directly compare their growth. The use of RGM medium alone without pre-treatment provided a sensitivity (91%) comparable to that offered by culture on both 7H10 and 7H11 with acid pretreatment (combined sensitivity; 86%) with significantly less overgrowth and interference from other organisms on RGM medium. The average concentration of NTM observed on RGM medium alone was comparable to or greater than the NTM concentration on either medium alone or combined. Thirty-three species were examined in parallel and all tested strains of 27 of these species successfully grew on RGM medium, including 19 of 21 from the CDC’s healthcare-associated infections species list. RGM medium was successful at recovering environmental NTM without a pre-treatment, greatly reducing labor and materials required to process samples. Simplification of culture processing for environmental NTM will allow for a better assessment of their presence in building water systems and the potential for reduced exposure of susceptible populations.
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spelling pubmed-79285222021-03-10 Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples Alexander, Kimberly J. Furlong, Jennifer L. Baron, Julianne L. Rihs, John D. Stephenson, Dominic Perry, John D. Stout, Janet E. PLoS One Research Article Nontuberculous mycobacteria (NTM) are waterborne pathogens commonly found in building water systems where they are a primary concern to vulnerable patient populations and can cause severe disease. The recovery of NTM from environmental samples can be a laborious undertaking and current pre-treatment methods and selective media lack sensitivity. We explored the use of the highly selective Rapidly Growing Mycobacteria (RGM) medium for culturing NTM from environmental water samples compared to existing methods. In total, 223 environmental water samples, including potable and non-potable water, were cultured for NTM using three culture media. In addition to direct culture on RGM medium, each sample was cultured on Middlebrook 7H10 medium and Mitchison 7H11 medium after pre-treatment with 0.2M KCl-HCl. Additionally, 33 distinct species of NTM were inoculated onto RGM medium and 7H10 medium in parallel to directly compare their growth. The use of RGM medium alone without pre-treatment provided a sensitivity (91%) comparable to that offered by culture on both 7H10 and 7H11 with acid pretreatment (combined sensitivity; 86%) with significantly less overgrowth and interference from other organisms on RGM medium. The average concentration of NTM observed on RGM medium alone was comparable to or greater than the NTM concentration on either medium alone or combined. Thirty-three species were examined in parallel and all tested strains of 27 of these species successfully grew on RGM medium, including 19 of 21 from the CDC’s healthcare-associated infections species list. RGM medium was successful at recovering environmental NTM without a pre-treatment, greatly reducing labor and materials required to process samples. Simplification of culture processing for environmental NTM will allow for a better assessment of their presence in building water systems and the potential for reduced exposure of susceptible populations. Public Library of Science 2021-03-03 /pmc/articles/PMC7928522/ /pubmed/33657154 http://dx.doi.org/10.1371/journal.pone.0247166 Text en © 2021 Alexander et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Alexander, Kimberly J.
Furlong, Jennifer L.
Baron, Julianne L.
Rihs, John D.
Stephenson, Dominic
Perry, John D.
Stout, Janet E.
Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples
title Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples
title_full Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples
title_fullStr Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples
title_full_unstemmed Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples
title_short Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples
title_sort evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928522/
https://www.ncbi.nlm.nih.gov/pubmed/33657154
http://dx.doi.org/10.1371/journal.pone.0247166
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