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miRNA-296-5p functions as a potential tumor suppressor in human osteosarcoma by targeting SND1

BACKGROUND: The pathogenesis of osteosarcoma (OS) is still unclear, and it is still necessary to find new targets and drugs for anti-OS. This study aimed to investigate the role and mechanism of the anti-OS effects of miR-296-5p. METHODS: We measured the expression of miR-296-5p in human OS cell lin...

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Autores principales: Huang, Ya-Zeng, Zhang, Jun, Shen, Jian-Jian, Zhao, Ting-Xiao, Xu, You-Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7929571/
https://www.ncbi.nlm.nih.gov/pubmed/33652459
http://dx.doi.org/10.1097/CM9.0000000000001400
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author Huang, Ya-Zeng
Zhang, Jun
Shen, Jian-Jian
Zhao, Ting-Xiao
Xu, You-Jia
author_facet Huang, Ya-Zeng
Zhang, Jun
Shen, Jian-Jian
Zhao, Ting-Xiao
Xu, You-Jia
author_sort Huang, Ya-Zeng
collection PubMed
description BACKGROUND: The pathogenesis of osteosarcoma (OS) is still unclear, and it is still necessary to find new targets and drugs for anti-OS. This study aimed to investigate the role and mechanism of the anti-OS effects of miR-296-5p. METHODS: We measured the expression of miR-296-5p in human OS cell lines and tissues. The effect of miR-296-5p and its target gene staphylococcal nuclease and tudor domain containing 1 on proliferation, migration, and invasion of human OS lines was examined. The Student's t test was used for statistical analysis. RESULTS: We found that microRNA (miR)-296-5p was significantly downregulated in OS cell lines and tissues (control vs. OS, 1.802 ± 0.313 vs. 0.618 ± 0.235, t = 6.402, P < 0.01). Overexpression of miR-296-5p suppressed proliferation, migration, and invasion of OA cells. SND1 was identified as a target of miR-296-5p by bioinformatic analysis and dual-luciferase reporter assay. Overexpression of SND1 abrogated the effects induced by miR-296-5p upregulation (miRNA-296-5p vs. miRNA-296-5p + SND1, 0.294 ± 0.159 vs. 2.300 ± 0.277, t = 12.68, P = 0.003). CONCLUSION: Our study indicates that miR-296-5p may function as a tumor suppressor by targeting SND1 in OS.
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spelling pubmed-79295712021-03-05 miRNA-296-5p functions as a potential tumor suppressor in human osteosarcoma by targeting SND1 Huang, Ya-Zeng Zhang, Jun Shen, Jian-Jian Zhao, Ting-Xiao Xu, You-Jia Chin Med J (Engl) Original Articles BACKGROUND: The pathogenesis of osteosarcoma (OS) is still unclear, and it is still necessary to find new targets and drugs for anti-OS. This study aimed to investigate the role and mechanism of the anti-OS effects of miR-296-5p. METHODS: We measured the expression of miR-296-5p in human OS cell lines and tissues. The effect of miR-296-5p and its target gene staphylococcal nuclease and tudor domain containing 1 on proliferation, migration, and invasion of human OS lines was examined. The Student's t test was used for statistical analysis. RESULTS: We found that microRNA (miR)-296-5p was significantly downregulated in OS cell lines and tissues (control vs. OS, 1.802 ± 0.313 vs. 0.618 ± 0.235, t = 6.402, P < 0.01). Overexpression of miR-296-5p suppressed proliferation, migration, and invasion of OA cells. SND1 was identified as a target of miR-296-5p by bioinformatic analysis and dual-luciferase reporter assay. Overexpression of SND1 abrogated the effects induced by miR-296-5p upregulation (miRNA-296-5p vs. miRNA-296-5p + SND1, 0.294 ± 0.159 vs. 2.300 ± 0.277, t = 12.68, P = 0.003). CONCLUSION: Our study indicates that miR-296-5p may function as a tumor suppressor by targeting SND1 in OS. Lippincott Williams & Wilkins 2021-03-05 2021-02-09 /pmc/articles/PMC7929571/ /pubmed/33652459 http://dx.doi.org/10.1097/CM9.0000000000001400 Text en Copyright © 2021 The Chinese Medical Association, produced by Wolters Kluwer, Inc. under the CC-BY-NC-ND license. http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0
spellingShingle Original Articles
Huang, Ya-Zeng
Zhang, Jun
Shen, Jian-Jian
Zhao, Ting-Xiao
Xu, You-Jia
miRNA-296-5p functions as a potential tumor suppressor in human osteosarcoma by targeting SND1
title miRNA-296-5p functions as a potential tumor suppressor in human osteosarcoma by targeting SND1
title_full miRNA-296-5p functions as a potential tumor suppressor in human osteosarcoma by targeting SND1
title_fullStr miRNA-296-5p functions as a potential tumor suppressor in human osteosarcoma by targeting SND1
title_full_unstemmed miRNA-296-5p functions as a potential tumor suppressor in human osteosarcoma by targeting SND1
title_short miRNA-296-5p functions as a potential tumor suppressor in human osteosarcoma by targeting SND1
title_sort mirna-296-5p functions as a potential tumor suppressor in human osteosarcoma by targeting snd1
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7929571/
https://www.ncbi.nlm.nih.gov/pubmed/33652459
http://dx.doi.org/10.1097/CM9.0000000000001400
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