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Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode

Inactivation technology for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is certainly a critical measure to mitigate the spread of coronavirus disease 2019 (COVID-19). A deep ultraviolet light-emitting diode (DUV-LED) would be a promising candidate to inactivate SARS-CoV-2, based on...

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Autores principales: Minamikawa, Takeo, Koma, Takaaki, Suzuki, Akihiro, Mizuno, Takahiko, Nagamatsu, Kentaro, Arimochi, Hideki, Tsuchiya, Koichiro, Matsuoka, Kaoru, Yasui, Takeshi, Yasutomo, Koji, Nomaguchi, Masako
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930116/
https://www.ncbi.nlm.nih.gov/pubmed/33658595
http://dx.doi.org/10.1038/s41598-021-84592-0
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author Minamikawa, Takeo
Koma, Takaaki
Suzuki, Akihiro
Mizuno, Takahiko
Nagamatsu, Kentaro
Arimochi, Hideki
Tsuchiya, Koichiro
Matsuoka, Kaoru
Yasui, Takeshi
Yasutomo, Koji
Nomaguchi, Masako
author_facet Minamikawa, Takeo
Koma, Takaaki
Suzuki, Akihiro
Mizuno, Takahiko
Nagamatsu, Kentaro
Arimochi, Hideki
Tsuchiya, Koichiro
Matsuoka, Kaoru
Yasui, Takeshi
Yasutomo, Koji
Nomaguchi, Masako
author_sort Minamikawa, Takeo
collection PubMed
description Inactivation technology for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is certainly a critical measure to mitigate the spread of coronavirus disease 2019 (COVID-19). A deep ultraviolet light-emitting diode (DUV-LED) would be a promising candidate to inactivate SARS-CoV-2, based on the well-known antiviral effects of DUV on microorganisms and viruses. However, due to variations in the inactivation effects across different viruses, quantitative evaluations of the inactivation profile of SARS-CoV-2 by DUV-LED irradiation need to be performed. In the present study, we quantify the irradiation dose of DUV-LED necessary to inactivate SARS-CoV-2. For this purpose, we determined the culture media suitable for the irradiation of SARS-CoV-2 and optimized the irradiation apparatus using commercially available DUV-LEDs that operate at a center wavelength of 265, 280, or 300 nm. Under these conditions, we successfully analyzed the relationship between SARS-CoV-2 infectivity and the irradiation dose of the DUV-LEDs at each wavelength without irrelevant biological effects. In conclusion, total doses of 1.8 mJ/cm(2) for 265 nm, 3.0 mJ/cm(2) for 280 nm, and 23 mJ/cm(2) for 300 nm are required to inactivate 99.9% of SARS-CoV-2. Our results provide quantitative antiviral effects of DUV irradiation on SARS-CoV-2, serving as basic knowledge of inactivation technologies against SARS-CoV-2.
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spelling pubmed-79301162021-03-05 Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode Minamikawa, Takeo Koma, Takaaki Suzuki, Akihiro Mizuno, Takahiko Nagamatsu, Kentaro Arimochi, Hideki Tsuchiya, Koichiro Matsuoka, Kaoru Yasui, Takeshi Yasutomo, Koji Nomaguchi, Masako Sci Rep Article Inactivation technology for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is certainly a critical measure to mitigate the spread of coronavirus disease 2019 (COVID-19). A deep ultraviolet light-emitting diode (DUV-LED) would be a promising candidate to inactivate SARS-CoV-2, based on the well-known antiviral effects of DUV on microorganisms and viruses. However, due to variations in the inactivation effects across different viruses, quantitative evaluations of the inactivation profile of SARS-CoV-2 by DUV-LED irradiation need to be performed. In the present study, we quantify the irradiation dose of DUV-LED necessary to inactivate SARS-CoV-2. For this purpose, we determined the culture media suitable for the irradiation of SARS-CoV-2 and optimized the irradiation apparatus using commercially available DUV-LEDs that operate at a center wavelength of 265, 280, or 300 nm. Under these conditions, we successfully analyzed the relationship between SARS-CoV-2 infectivity and the irradiation dose of the DUV-LEDs at each wavelength without irrelevant biological effects. In conclusion, total doses of 1.8 mJ/cm(2) for 265 nm, 3.0 mJ/cm(2) for 280 nm, and 23 mJ/cm(2) for 300 nm are required to inactivate 99.9% of SARS-CoV-2. Our results provide quantitative antiviral effects of DUV irradiation on SARS-CoV-2, serving as basic knowledge of inactivation technologies against SARS-CoV-2. Nature Publishing Group UK 2021-03-03 /pmc/articles/PMC7930116/ /pubmed/33658595 http://dx.doi.org/10.1038/s41598-021-84592-0 Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Minamikawa, Takeo
Koma, Takaaki
Suzuki, Akihiro
Mizuno, Takahiko
Nagamatsu, Kentaro
Arimochi, Hideki
Tsuchiya, Koichiro
Matsuoka, Kaoru
Yasui, Takeshi
Yasutomo, Koji
Nomaguchi, Masako
Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode
title Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode
title_full Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode
title_fullStr Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode
title_full_unstemmed Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode
title_short Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode
title_sort quantitative evaluation of sars-cov-2 inactivation using a deep ultraviolet light-emitting diode
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930116/
https://www.ncbi.nlm.nih.gov/pubmed/33658595
http://dx.doi.org/10.1038/s41598-021-84592-0
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