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Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13

Bacteria secrete and utilize nanoparticles, called extracellular membrane vesicles (EMVs), for survival in their growing environments. Therefore, the amount and components of EMVs should be tuned in response to the environment. However, how bacteria regulate vesiculation in response to the extracell...

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Autores principales: Yokoyama, Fumiaki, Imai, Tomoya, Aoki, Wataru, Ueda, Mitsuyoshi, Kawamoto, Jun, Kurihara, Tatsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930318/
https://www.ncbi.nlm.nih.gov/pubmed/33679653
http://dx.doi.org/10.3389/fmicb.2021.629023
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author Yokoyama, Fumiaki
Imai, Tomoya
Aoki, Wataru
Ueda, Mitsuyoshi
Kawamoto, Jun
Kurihara, Tatsuo
author_facet Yokoyama, Fumiaki
Imai, Tomoya
Aoki, Wataru
Ueda, Mitsuyoshi
Kawamoto, Jun
Kurihara, Tatsuo
author_sort Yokoyama, Fumiaki
collection PubMed
description Bacteria secrete and utilize nanoparticles, called extracellular membrane vesicles (EMVs), for survival in their growing environments. Therefore, the amount and components of EMVs should be tuned in response to the environment. However, how bacteria regulate vesiculation in response to the extracellular environment remains largely unknown. In this study, we identified a putative sensor protein, HM1275, involved in the induction of vesicle production at high lysine concentration in a hypervesiculating Gram-negative bacterium, Shewanella vesiculosa HM13. This protein was predicted to possess typical sensing and signaling domains of sensor proteins, such as methyl-accepting chemotaxis proteins. Comparison of vesicle production between the hm1275-disrupted mutant and the parent strain revealed that HM1275 is involved in lysine-induced hypervesiculation. Moreover, HM1275 has sequence similarity to a biofilm dispersion protein, BdlA, of Pseudomonas aeruginosa PAO1, and hm1275 disruption increased the amount of biofilm. Thus, this study showed that the induction of vesicle production and suppression of biofilm formation in response to lysine concentration are under the control of the same putative sensor protein.
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spelling pubmed-79303182021-03-05 Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13 Yokoyama, Fumiaki Imai, Tomoya Aoki, Wataru Ueda, Mitsuyoshi Kawamoto, Jun Kurihara, Tatsuo Front Microbiol Microbiology Bacteria secrete and utilize nanoparticles, called extracellular membrane vesicles (EMVs), for survival in their growing environments. Therefore, the amount and components of EMVs should be tuned in response to the environment. However, how bacteria regulate vesiculation in response to the extracellular environment remains largely unknown. In this study, we identified a putative sensor protein, HM1275, involved in the induction of vesicle production at high lysine concentration in a hypervesiculating Gram-negative bacterium, Shewanella vesiculosa HM13. This protein was predicted to possess typical sensing and signaling domains of sensor proteins, such as methyl-accepting chemotaxis proteins. Comparison of vesicle production between the hm1275-disrupted mutant and the parent strain revealed that HM1275 is involved in lysine-induced hypervesiculation. Moreover, HM1275 has sequence similarity to a biofilm dispersion protein, BdlA, of Pseudomonas aeruginosa PAO1, and hm1275 disruption increased the amount of biofilm. Thus, this study showed that the induction of vesicle production and suppression of biofilm formation in response to lysine concentration are under the control of the same putative sensor protein. Frontiers Media S.A. 2021-02-18 /pmc/articles/PMC7930318/ /pubmed/33679653 http://dx.doi.org/10.3389/fmicb.2021.629023 Text en Copyright © 2021 Yokoyama, Imai, Aoki, Ueda, Kawamoto and Kurihara. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yokoyama, Fumiaki
Imai, Tomoya
Aoki, Wataru
Ueda, Mitsuyoshi
Kawamoto, Jun
Kurihara, Tatsuo
Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13
title Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13
title_full Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13
title_fullStr Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13
title_full_unstemmed Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13
title_short Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13
title_sort identification of a putative sensor protein involved in regulation of vesicle production by a hypervesiculating bacterium, shewanella vesiculosa hm13
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930318/
https://www.ncbi.nlm.nih.gov/pubmed/33679653
http://dx.doi.org/10.3389/fmicb.2021.629023
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