Cargando…
Artesunate and Dihydroartemisinin Inhibit Rabies Virus Replication
Rabies is caused by infection of rabies virus (RABV) and remains a serious threat to the global public health. Except for the requirement for cold chain and high cost of human rabies immune globulin, no small molecule drugs are currently available for clinical treatment of rabies. So, it is of great...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Singapore
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930525/ https://www.ncbi.nlm.nih.gov/pubmed/33661488 http://dx.doi.org/10.1007/s12250-021-00349-z |
_version_ | 1783660118958669824 |
---|---|
author | Luo, Jun Zhang, Yue Wang, Yang Liu, Qing Li, Jiesen He, Hongling Luo, Yongwen Huang, Shile Guo, Xiaofeng |
author_facet | Luo, Jun Zhang, Yue Wang, Yang Liu, Qing Li, Jiesen He, Hongling Luo, Yongwen Huang, Shile Guo, Xiaofeng |
author_sort | Luo, Jun |
collection | PubMed |
description | Rabies is caused by infection of rabies virus (RABV) and remains a serious threat to the global public health. Except for the requirement for cold chain and high cost of human rabies immune globulin, no small molecule drugs are currently available for clinical treatment of rabies. So, it is of great importance to identify novel compounds that can effectively inhibit RABV infection. Artesunate (ART) and dihydroartemisinin (DHA), two derivatives of artemisinin, are widely used for treatment of malaria in adults and children, showing high safety. In this study, we found that both ART and DHA were able to inhibit RABV replication in host cells at a low concentration (0.1 μmol/L). The antiviral effects of ART and DHA were independent of viral strains and cell lines. Pre-treatment with ART or DHA for 2 h in vitro did not affect the viral replication in host cells, implying that ART and DHA neither reduced the viability of RABV directly nor inhibited the binding and entrance of the virus to host cells. Further studies revealed that ART and DHA inhibited RABV genomic RNA synthesis and viral gene transcription. Treatment with ART or DHA (5 mg/kg) by intramuscular injection improved, to some extent, the survival rate of RABV-challenged mice. Combination treatment with derivatives of artemisinin and mannitol significantly improved the survival rate of RABV-challenged mice. The results suggest that ART and DHA have a great potential to be explored as new anti-rabies agents for treatment of rabies. |
format | Online Article Text |
id | pubmed-7930525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-79305252021-03-04 Artesunate and Dihydroartemisinin Inhibit Rabies Virus Replication Luo, Jun Zhang, Yue Wang, Yang Liu, Qing Li, Jiesen He, Hongling Luo, Yongwen Huang, Shile Guo, Xiaofeng Virol Sin Research Article Rabies is caused by infection of rabies virus (RABV) and remains a serious threat to the global public health. Except for the requirement for cold chain and high cost of human rabies immune globulin, no small molecule drugs are currently available for clinical treatment of rabies. So, it is of great importance to identify novel compounds that can effectively inhibit RABV infection. Artesunate (ART) and dihydroartemisinin (DHA), two derivatives of artemisinin, are widely used for treatment of malaria in adults and children, showing high safety. In this study, we found that both ART and DHA were able to inhibit RABV replication in host cells at a low concentration (0.1 μmol/L). The antiviral effects of ART and DHA were independent of viral strains and cell lines. Pre-treatment with ART or DHA for 2 h in vitro did not affect the viral replication in host cells, implying that ART and DHA neither reduced the viability of RABV directly nor inhibited the binding and entrance of the virus to host cells. Further studies revealed that ART and DHA inhibited RABV genomic RNA synthesis and viral gene transcription. Treatment with ART or DHA (5 mg/kg) by intramuscular injection improved, to some extent, the survival rate of RABV-challenged mice. Combination treatment with derivatives of artemisinin and mannitol significantly improved the survival rate of RABV-challenged mice. The results suggest that ART and DHA have a great potential to be explored as new anti-rabies agents for treatment of rabies. Springer Singapore 2021-03-04 /pmc/articles/PMC7930525/ /pubmed/33661488 http://dx.doi.org/10.1007/s12250-021-00349-z Text en © Wuhan Institute of Virology, CAS 2021 |
spellingShingle | Research Article Luo, Jun Zhang, Yue Wang, Yang Liu, Qing Li, Jiesen He, Hongling Luo, Yongwen Huang, Shile Guo, Xiaofeng Artesunate and Dihydroartemisinin Inhibit Rabies Virus Replication |
title | Artesunate and Dihydroartemisinin Inhibit Rabies Virus Replication |
title_full | Artesunate and Dihydroartemisinin Inhibit Rabies Virus Replication |
title_fullStr | Artesunate and Dihydroartemisinin Inhibit Rabies Virus Replication |
title_full_unstemmed | Artesunate and Dihydroartemisinin Inhibit Rabies Virus Replication |
title_short | Artesunate and Dihydroartemisinin Inhibit Rabies Virus Replication |
title_sort | artesunate and dihydroartemisinin inhibit rabies virus replication |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930525/ https://www.ncbi.nlm.nih.gov/pubmed/33661488 http://dx.doi.org/10.1007/s12250-021-00349-z |
work_keys_str_mv | AT luojun artesunateanddihydroartemisinininhibitrabiesvirusreplication AT zhangyue artesunateanddihydroartemisinininhibitrabiesvirusreplication AT wangyang artesunateanddihydroartemisinininhibitrabiesvirusreplication AT liuqing artesunateanddihydroartemisinininhibitrabiesvirusreplication AT lijiesen artesunateanddihydroartemisinininhibitrabiesvirusreplication AT hehongling artesunateanddihydroartemisinininhibitrabiesvirusreplication AT luoyongwen artesunateanddihydroartemisinininhibitrabiesvirusreplication AT huangshile artesunateanddihydroartemisinininhibitrabiesvirusreplication AT guoxiaofeng artesunateanddihydroartemisinininhibitrabiesvirusreplication |