Cargando…

Expression and purification of fused kinase from insect cells for in vitro kinase assay

The Fused (Fu) kinase is a key transducer of Hedgehog signaling, but its relevant substrates have remained obscured due to the difficulty of obtaining active Fu for in vitro kinase assay. Based on the mechanism of Fu activation in vivo, we engineered a constitutively active Fu and expressed it in Sf...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Yuhong, Jiang, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930607/
https://www.ncbi.nlm.nih.gov/pubmed/33681825
http://dx.doi.org/10.1016/j.xpro.2021.100376