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Dither removing Fourier ptychographic microscope based on a two-axis rotation stage
Significance: Large space-bandwidth product is highly desirable in many biomedical imaging. Fourier ptychographic microscopy (FPM) is a computational imaging technique that can significantly increase the space-bandwidth product of a standard microscope. The illuminator of a Fourier ptychographic mic...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society of Photo-Optical Instrumentation Engineers
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930810/ https://www.ncbi.nlm.nih.gov/pubmed/33665992 http://dx.doi.org/10.1117/1.JBO.26.3.036501 |
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author | Huang, Kaicheng Hui, Wangwei Ye, Qing Zhao, Hongyang Shi, Qiushuai Tian, Jianguo Zhou, Wenyuan |
author_facet | Huang, Kaicheng Hui, Wangwei Ye, Qing Zhao, Hongyang Shi, Qiushuai Tian, Jianguo Zhou, Wenyuan |
author_sort | Huang, Kaicheng |
collection | PubMed |
description | Significance: Large space-bandwidth product is highly desirable in many biomedical imaging. Fourier ptychographic microscopy (FPM) is a computational imaging technique that can significantly increase the space-bandwidth product of a standard microscope. The illuminator of a Fourier ptychographic microscope is not flexible at present, and it is inconvenient to meet different imaging needs. Aim: An illuminator based on a two-axis motorized rotation stage was presented to provide a more flexible illuminating way with the goal of meeting different imaging needs. Approach: The illuminator adopts a concentric illuminating method to provide coherent illumination in any direction on the sample plane. The sampling pattern can be freely designed and changed according to the parameters of the imaging system. A dither removing algorithm was proposed to remove the potential dither influence introduced in the image acquisition process. Results: The illuminator could be conveniently integrated into different imaging systems. The feasibility and flexibility were demonstrated by applying it to imaging systems with numerical aperture of 0.045 and 0.01. The resolution gain is about 4- and 13-fold, respectively. The effectiveness of the dither removing algorithm was validated in both simulation and experiment. Conclusions: A more flexible illuminator for FPM was presented to meet different imaging needs. A dither removing algorithm was proposed to remove dither influence. |
format | Online Article Text |
id | pubmed-7930810 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Society of Photo-Optical Instrumentation Engineers |
record_format | MEDLINE/PubMed |
spelling | pubmed-79308102021-03-04 Dither removing Fourier ptychographic microscope based on a two-axis rotation stage Huang, Kaicheng Hui, Wangwei Ye, Qing Zhao, Hongyang Shi, Qiushuai Tian, Jianguo Zhou, Wenyuan J Biomed Opt Microscopy Significance: Large space-bandwidth product is highly desirable in many biomedical imaging. Fourier ptychographic microscopy (FPM) is a computational imaging technique that can significantly increase the space-bandwidth product of a standard microscope. The illuminator of a Fourier ptychographic microscope is not flexible at present, and it is inconvenient to meet different imaging needs. Aim: An illuminator based on a two-axis motorized rotation stage was presented to provide a more flexible illuminating way with the goal of meeting different imaging needs. Approach: The illuminator adopts a concentric illuminating method to provide coherent illumination in any direction on the sample plane. The sampling pattern can be freely designed and changed according to the parameters of the imaging system. A dither removing algorithm was proposed to remove the potential dither influence introduced in the image acquisition process. Results: The illuminator could be conveniently integrated into different imaging systems. The feasibility and flexibility were demonstrated by applying it to imaging systems with numerical aperture of 0.045 and 0.01. The resolution gain is about 4- and 13-fold, respectively. The effectiveness of the dither removing algorithm was validated in both simulation and experiment. Conclusions: A more flexible illuminator for FPM was presented to meet different imaging needs. A dither removing algorithm was proposed to remove dither influence. Society of Photo-Optical Instrumentation Engineers 2021-03-04 2021-03 /pmc/articles/PMC7930810/ /pubmed/33665992 http://dx.doi.org/10.1117/1.JBO.26.3.036501 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/ Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
spellingShingle | Microscopy Huang, Kaicheng Hui, Wangwei Ye, Qing Zhao, Hongyang Shi, Qiushuai Tian, Jianguo Zhou, Wenyuan Dither removing Fourier ptychographic microscope based on a two-axis rotation stage |
title | Dither removing Fourier ptychographic microscope based on a two-axis rotation stage |
title_full | Dither removing Fourier ptychographic microscope based on a two-axis rotation stage |
title_fullStr | Dither removing Fourier ptychographic microscope based on a two-axis rotation stage |
title_full_unstemmed | Dither removing Fourier ptychographic microscope based on a two-axis rotation stage |
title_short | Dither removing Fourier ptychographic microscope based on a two-axis rotation stage |
title_sort | dither removing fourier ptychographic microscope based on a two-axis rotation stage |
topic | Microscopy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930810/ https://www.ncbi.nlm.nih.gov/pubmed/33665992 http://dx.doi.org/10.1117/1.JBO.26.3.036501 |
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