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Effects of Process Parameters on Structure and Properties of Melt-Blown Poly(Lactic Acid) Nonwovens for Skin Regeneration

Skin regeneration requires a three-dimensional (3D) scaffold for cell adhesion, growth and proliferation. A type of the scaffold offering a 3D structure is a nonwoven material produced via a melt-blown technique. Process parameters of this technique can be adapted to improve the cellular response. P...

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Autores principales: Dzierzkowska, Ewa, Scisłowska-Czarnecka, Anna, Kudzin, Marcin, Boguń, Maciej, Szatkowski, Piotr, Gajek, Marcin, Kornaus, Kamil, Chadzinska, Magdalena, Stodolak-Zych, Ewa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931000/
https://www.ncbi.nlm.nih.gov/pubmed/33652627
http://dx.doi.org/10.3390/jfb12010016
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author Dzierzkowska, Ewa
Scisłowska-Czarnecka, Anna
Kudzin, Marcin
Boguń, Maciej
Szatkowski, Piotr
Gajek, Marcin
Kornaus, Kamil
Chadzinska, Magdalena
Stodolak-Zych, Ewa
author_facet Dzierzkowska, Ewa
Scisłowska-Czarnecka, Anna
Kudzin, Marcin
Boguń, Maciej
Szatkowski, Piotr
Gajek, Marcin
Kornaus, Kamil
Chadzinska, Magdalena
Stodolak-Zych, Ewa
author_sort Dzierzkowska, Ewa
collection PubMed
description Skin regeneration requires a three-dimensional (3D) scaffold for cell adhesion, growth and proliferation. A type of the scaffold offering a 3D structure is a nonwoven material produced via a melt-blown technique. Process parameters of this technique can be adapted to improve the cellular response. Polylactic acid (PLA) was used to produce a nonwoven scaffold by a melt-blown technique. The key process parameters, i.e., the head and air temperature, were changed in the range from 180–270 °C to obtain eight different materials (MB1–MB8). The relationships between the process parameters, morphology, porosity, thermal properties and the cellular response were explored in this study. The mean fiber diameters ranged from 3 to 120 µm. The average material roughness values were between 47 and 160 µm, whereas the pore diameters ranged from 5 to 400 µm. The calorimetry thermograms revealed a correlation between the temperature parameters and crystallization. The response of keratinocytes and macrophages exhibited a higher cell viability on thicker fibers. The cell-scaffold interaction was observed via SEM after 7 days. This result proved that the features of melt-blown nonwoven scaffolds depended on the processing parameters, such as head temperature and air temperature. Thanks to examinations, the most suitable scaffolds for skin tissue regeneration were selected.
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spelling pubmed-79310002021-03-05 Effects of Process Parameters on Structure and Properties of Melt-Blown Poly(Lactic Acid) Nonwovens for Skin Regeneration Dzierzkowska, Ewa Scisłowska-Czarnecka, Anna Kudzin, Marcin Boguń, Maciej Szatkowski, Piotr Gajek, Marcin Kornaus, Kamil Chadzinska, Magdalena Stodolak-Zych, Ewa J Funct Biomater Article Skin regeneration requires a three-dimensional (3D) scaffold for cell adhesion, growth and proliferation. A type of the scaffold offering a 3D structure is a nonwoven material produced via a melt-blown technique. Process parameters of this technique can be adapted to improve the cellular response. Polylactic acid (PLA) was used to produce a nonwoven scaffold by a melt-blown technique. The key process parameters, i.e., the head and air temperature, were changed in the range from 180–270 °C to obtain eight different materials (MB1–MB8). The relationships between the process parameters, morphology, porosity, thermal properties and the cellular response were explored in this study. The mean fiber diameters ranged from 3 to 120 µm. The average material roughness values were between 47 and 160 µm, whereas the pore diameters ranged from 5 to 400 µm. The calorimetry thermograms revealed a correlation between the temperature parameters and crystallization. The response of keratinocytes and macrophages exhibited a higher cell viability on thicker fibers. The cell-scaffold interaction was observed via SEM after 7 days. This result proved that the features of melt-blown nonwoven scaffolds depended on the processing parameters, such as head temperature and air temperature. Thanks to examinations, the most suitable scaffolds for skin tissue regeneration were selected. MDPI 2021-02-26 /pmc/articles/PMC7931000/ /pubmed/33652627 http://dx.doi.org/10.3390/jfb12010016 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dzierzkowska, Ewa
Scisłowska-Czarnecka, Anna
Kudzin, Marcin
Boguń, Maciej
Szatkowski, Piotr
Gajek, Marcin
Kornaus, Kamil
Chadzinska, Magdalena
Stodolak-Zych, Ewa
Effects of Process Parameters on Structure and Properties of Melt-Blown Poly(Lactic Acid) Nonwovens for Skin Regeneration
title Effects of Process Parameters on Structure and Properties of Melt-Blown Poly(Lactic Acid) Nonwovens for Skin Regeneration
title_full Effects of Process Parameters on Structure and Properties of Melt-Blown Poly(Lactic Acid) Nonwovens for Skin Regeneration
title_fullStr Effects of Process Parameters on Structure and Properties of Melt-Blown Poly(Lactic Acid) Nonwovens for Skin Regeneration
title_full_unstemmed Effects of Process Parameters on Structure and Properties of Melt-Blown Poly(Lactic Acid) Nonwovens for Skin Regeneration
title_short Effects of Process Parameters on Structure and Properties of Melt-Blown Poly(Lactic Acid) Nonwovens for Skin Regeneration
title_sort effects of process parameters on structure and properties of melt-blown poly(lactic acid) nonwovens for skin regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931000/
https://www.ncbi.nlm.nih.gov/pubmed/33652627
http://dx.doi.org/10.3390/jfb12010016
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