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Depletion of CTCF disrupts PSG gene expression in the human trophoblast cell line Swan 71
Pregnancy‐specific glycoproteins (PSGs) are fetal proteins secreted by the placenta during pregnancy. The PSG level in maternal serum is an indicator of risk for pregnancy complications. However, little is known about the molecular mechanisms underlying PSG gene expression. Recently, the importance...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931220/ https://www.ncbi.nlm.nih.gov/pubmed/33452729 http://dx.doi.org/10.1002/2211-5463.13087 |
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author | Jeong, Da Som Kim, Myoung Hee Lee, Ji‐Yeon |
author_facet | Jeong, Da Som Kim, Myoung Hee Lee, Ji‐Yeon |
author_sort | Jeong, Da Som |
collection | PubMed |
description | Pregnancy‐specific glycoproteins (PSGs) are fetal proteins secreted by the placenta during pregnancy. The PSG level in maternal serum is an indicator of risk for pregnancy complications. However, little is known about the molecular mechanisms underlying PSG gene expression. Recently, the importance of epigenetic regulation of placental genes has been emphasized in the study of developmental defects and placental disease. In this study, the role of the CCCTC‐binding factor (CTCF) in regulation of PSG expression was investigated to better understand the epigenetic regulatory mechanisms of the PSG genes. Inhibition of CTCF expression disturbed transcription of several PSG genes: PSG1, PSG2, PSG4, PSG5, PSG8, and PSG9 were upregulated and PSG6 and PSG11 were downregulated. These transcriptional changes were correlated with decreased CTCF binding and changes in histone modification at the PSG promoters. Our data demonstrate that CTCF is a potential mediator in the regulation of PSG gene expression. |
format | Online Article Text |
id | pubmed-7931220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79312202021-03-15 Depletion of CTCF disrupts PSG gene expression in the human trophoblast cell line Swan 71 Jeong, Da Som Kim, Myoung Hee Lee, Ji‐Yeon FEBS Open Bio Research Articles Pregnancy‐specific glycoproteins (PSGs) are fetal proteins secreted by the placenta during pregnancy. The PSG level in maternal serum is an indicator of risk for pregnancy complications. However, little is known about the molecular mechanisms underlying PSG gene expression. Recently, the importance of epigenetic regulation of placental genes has been emphasized in the study of developmental defects and placental disease. In this study, the role of the CCCTC‐binding factor (CTCF) in regulation of PSG expression was investigated to better understand the epigenetic regulatory mechanisms of the PSG genes. Inhibition of CTCF expression disturbed transcription of several PSG genes: PSG1, PSG2, PSG4, PSG5, PSG8, and PSG9 were upregulated and PSG6 and PSG11 were downregulated. These transcriptional changes were correlated with decreased CTCF binding and changes in histone modification at the PSG promoters. Our data demonstrate that CTCF is a potential mediator in the regulation of PSG gene expression. John Wiley and Sons Inc. 2021-03-02 /pmc/articles/PMC7931220/ /pubmed/33452729 http://dx.doi.org/10.1002/2211-5463.13087 Text en © 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Jeong, Da Som Kim, Myoung Hee Lee, Ji‐Yeon Depletion of CTCF disrupts PSG gene expression in the human trophoblast cell line Swan 71 |
title | Depletion of CTCF disrupts PSG gene expression in the human trophoblast cell line Swan 71 |
title_full | Depletion of CTCF disrupts PSG gene expression in the human trophoblast cell line Swan 71 |
title_fullStr | Depletion of CTCF disrupts PSG gene expression in the human trophoblast cell line Swan 71 |
title_full_unstemmed | Depletion of CTCF disrupts PSG gene expression in the human trophoblast cell line Swan 71 |
title_short | Depletion of CTCF disrupts PSG gene expression in the human trophoblast cell line Swan 71 |
title_sort | depletion of ctcf disrupts psg gene expression in the human trophoblast cell line swan 71 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931220/ https://www.ncbi.nlm.nih.gov/pubmed/33452729 http://dx.doi.org/10.1002/2211-5463.13087 |
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