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Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis
BACKGROUND: Studies have found that circular RNAs (circRNAs) play key roles in cardiovascular diseases. However, the function of circROBO2 in acute myocardial infarction (AMI) is unclear. This study aimed to investigate the pathogenesis of circROBO2 in AMI. METHODS: qRT-PCR and Western blot were use...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931513/ https://www.ncbi.nlm.nih.gov/pubmed/33658002 http://dx.doi.org/10.1186/s10020-021-00275-6 |
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author | Chen, Tian-ping Zhang, Nai-ju Wang, Hong-ju Hu, Si-gan Geng, Xu |
author_facet | Chen, Tian-ping Zhang, Nai-ju Wang, Hong-ju Hu, Si-gan Geng, Xu |
author_sort | Chen, Tian-ping |
collection | PubMed |
description | BACKGROUND: Studies have found that circular RNAs (circRNAs) play key roles in cardiovascular diseases. However, the function of circROBO2 in acute myocardial infarction (AMI) is unclear. This study aimed to investigate the pathogenesis of circROBO2 in AMI. METHODS: qRT-PCR and Western blot were used to determine the expression levels of circROBO2, miR-1184, and TRADD in AMI and sham-operated mouse models at mRNA and protein level, respectively. The relationship among miR-1184, circROBO2 and TRADD was evaluated by RNA immunoprecipitation (RIP) analysis and luciferase reporter gene analysis. The roles of circROBO2, miR-1184, and TRADD in myocardial cell apoptosis were evaluated using flow cytometry. Ultrasound echocardiography, serum creatine kinase MB (CK-MB) and lactate dehydrogenase (LDH), myocardial infarction area, and myocardial cell apoptosis were measured to examine the effects of circROBO2 on myocardial injury. RESULTS: The expression levels of miR-1184 were significantly reduced, and the expression levels of circROBO2 and TRADD were significantly increased in MI group. CircROBO2 acted as a sponge for miR-1184 by upregulating the expression of TRADD. In addition, overexpression of miR-1184 enhanced the protective effect of knockdown of circROBO2 by partially inhibiting the expression of TRADD in vivo and in vitro. CONCLUSION: Knockdown of circROBO2 reduced the apoptosis of cardiomyocytes by increasing the expression levels of miR-1184, which in turn decreased the expression levels of TRADD in the myocardium post-MI. |
format | Online Article Text |
id | pubmed-7931513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-79315132021-03-04 Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis Chen, Tian-ping Zhang, Nai-ju Wang, Hong-ju Hu, Si-gan Geng, Xu Mol Med Research Article BACKGROUND: Studies have found that circular RNAs (circRNAs) play key roles in cardiovascular diseases. However, the function of circROBO2 in acute myocardial infarction (AMI) is unclear. This study aimed to investigate the pathogenesis of circROBO2 in AMI. METHODS: qRT-PCR and Western blot were used to determine the expression levels of circROBO2, miR-1184, and TRADD in AMI and sham-operated mouse models at mRNA and protein level, respectively. The relationship among miR-1184, circROBO2 and TRADD was evaluated by RNA immunoprecipitation (RIP) analysis and luciferase reporter gene analysis. The roles of circROBO2, miR-1184, and TRADD in myocardial cell apoptosis were evaluated using flow cytometry. Ultrasound echocardiography, serum creatine kinase MB (CK-MB) and lactate dehydrogenase (LDH), myocardial infarction area, and myocardial cell apoptosis were measured to examine the effects of circROBO2 on myocardial injury. RESULTS: The expression levels of miR-1184 were significantly reduced, and the expression levels of circROBO2 and TRADD were significantly increased in MI group. CircROBO2 acted as a sponge for miR-1184 by upregulating the expression of TRADD. In addition, overexpression of miR-1184 enhanced the protective effect of knockdown of circROBO2 by partially inhibiting the expression of TRADD in vivo and in vitro. CONCLUSION: Knockdown of circROBO2 reduced the apoptosis of cardiomyocytes by increasing the expression levels of miR-1184, which in turn decreased the expression levels of TRADD in the myocardium post-MI. BioMed Central 2021-03-03 /pmc/articles/PMC7931513/ /pubmed/33658002 http://dx.doi.org/10.1186/s10020-021-00275-6 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Chen, Tian-ping Zhang, Nai-ju Wang, Hong-ju Hu, Si-gan Geng, Xu Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis |
title | Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis |
title_full | Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis |
title_fullStr | Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis |
title_full_unstemmed | Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis |
title_short | Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis |
title_sort | knockdown of circrobo2 attenuates acute myocardial infarction through regulating the mir-1184/tradd axis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931513/ https://www.ncbi.nlm.nih.gov/pubmed/33658002 http://dx.doi.org/10.1186/s10020-021-00275-6 |
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