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Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes
Polyhydroxyalkanoates (PHAs) production at pilot scale has been recently investigated and carried out exploiting different process configurations and organic wastes. More in detail, three pilot platforms, in Treviso (North-East of Italy), Carbonera (North-East of Italy) and Lisbon, produced PHAs by...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931994/ https://www.ncbi.nlm.nih.gov/pubmed/33681164 http://dx.doi.org/10.3389/fbioe.2021.628719 |
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author | Lorini, Laura Martinelli, Andrea Capuani, Giorgio Frison, Nicola Reis, Maria Sommer Ferreira, Bruno Villano, Marianna Majone, Mauro Valentino, Francesco |
author_facet | Lorini, Laura Martinelli, Andrea Capuani, Giorgio Frison, Nicola Reis, Maria Sommer Ferreira, Bruno Villano, Marianna Majone, Mauro Valentino, Francesco |
author_sort | Lorini, Laura |
collection | PubMed |
description | Polyhydroxyalkanoates (PHAs) production at pilot scale has been recently investigated and carried out exploiting different process configurations and organic wastes. More in detail, three pilot platforms, in Treviso (North-East of Italy), Carbonera (North-East of Italy) and Lisbon, produced PHAs by open mixed microbial cultures (MMCs) and different organic waste streams: organic fraction of municipal solid waste and sewage sludge (OFMSW-WAS), cellulosic primary sludge (CPS), and fruit waste (FW), respectively. In this context, two stabilization methods have been applied, and compared, for preserving the amount of PHA inside the cells: thermal drying and wet acidification of the biomass at the end of PHA accumulation process. Afterward, polymer has been extracted following an optimized method based on aqueous-phase inorganic reagents. Several PHA samples were then characterized to determine PHA purity, chemical composition, molecular weight, and thermal properties. The polymer contained two types of monomers, namely 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) at a relative percentage of 92.6–79.8 and 7.4–20.2 w/w, respectively, for Treviso and Lisbon plants. On the other hand, an opposite range was found for 3HB and 3HV monomers of PHA from Carbonera, which is 44.0–13.0 and 56.0–87.0 w/w, respectively. PHA extracted from wet-acidified biomass had generally higher viscosity average molecular weights (M(v)) (on average 424.8 ± 20.6 and 224.9 ± 21.9 KDa, respectively, for Treviso and Lisbon) while PHA recovered from thermally stabilized dried biomass had a three-fold lower M(v). |
format | Online Article Text |
id | pubmed-7931994 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79319942021-03-05 Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes Lorini, Laura Martinelli, Andrea Capuani, Giorgio Frison, Nicola Reis, Maria Sommer Ferreira, Bruno Villano, Marianna Majone, Mauro Valentino, Francesco Front Bioeng Biotechnol Bioengineering and Biotechnology Polyhydroxyalkanoates (PHAs) production at pilot scale has been recently investigated and carried out exploiting different process configurations and organic wastes. More in detail, three pilot platforms, in Treviso (North-East of Italy), Carbonera (North-East of Italy) and Lisbon, produced PHAs by open mixed microbial cultures (MMCs) and different organic waste streams: organic fraction of municipal solid waste and sewage sludge (OFMSW-WAS), cellulosic primary sludge (CPS), and fruit waste (FW), respectively. In this context, two stabilization methods have been applied, and compared, for preserving the amount of PHA inside the cells: thermal drying and wet acidification of the biomass at the end of PHA accumulation process. Afterward, polymer has been extracted following an optimized method based on aqueous-phase inorganic reagents. Several PHA samples were then characterized to determine PHA purity, chemical composition, molecular weight, and thermal properties. The polymer contained two types of monomers, namely 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) at a relative percentage of 92.6–79.8 and 7.4–20.2 w/w, respectively, for Treviso and Lisbon plants. On the other hand, an opposite range was found for 3HB and 3HV monomers of PHA from Carbonera, which is 44.0–13.0 and 56.0–87.0 w/w, respectively. PHA extracted from wet-acidified biomass had generally higher viscosity average molecular weights (M(v)) (on average 424.8 ± 20.6 and 224.9 ± 21.9 KDa, respectively, for Treviso and Lisbon) while PHA recovered from thermally stabilized dried biomass had a three-fold lower M(v). Frontiers Media S.A. 2021-02-18 /pmc/articles/PMC7931994/ /pubmed/33681164 http://dx.doi.org/10.3389/fbioe.2021.628719 Text en Copyright © 2021 Lorini, Martinelli, Capuani, Frison, Reis, Sommer Ferreira, Villano, Majone and Valentino. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Lorini, Laura Martinelli, Andrea Capuani, Giorgio Frison, Nicola Reis, Maria Sommer Ferreira, Bruno Villano, Marianna Majone, Mauro Valentino, Francesco Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes |
title | Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes |
title_full | Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes |
title_fullStr | Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes |
title_full_unstemmed | Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes |
title_short | Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes |
title_sort | characterization of polyhydroxyalkanoates produced at pilot scale from different organic wastes |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931994/ https://www.ncbi.nlm.nih.gov/pubmed/33681164 http://dx.doi.org/10.3389/fbioe.2021.628719 |
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