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Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes

Polyhydroxyalkanoates (PHAs) production at pilot scale has been recently investigated and carried out exploiting different process configurations and organic wastes. More in detail, three pilot platforms, in Treviso (North-East of Italy), Carbonera (North-East of Italy) and Lisbon, produced PHAs by...

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Autores principales: Lorini, Laura, Martinelli, Andrea, Capuani, Giorgio, Frison, Nicola, Reis, Maria, Sommer Ferreira, Bruno, Villano, Marianna, Majone, Mauro, Valentino, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931994/
https://www.ncbi.nlm.nih.gov/pubmed/33681164
http://dx.doi.org/10.3389/fbioe.2021.628719
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author Lorini, Laura
Martinelli, Andrea
Capuani, Giorgio
Frison, Nicola
Reis, Maria
Sommer Ferreira, Bruno
Villano, Marianna
Majone, Mauro
Valentino, Francesco
author_facet Lorini, Laura
Martinelli, Andrea
Capuani, Giorgio
Frison, Nicola
Reis, Maria
Sommer Ferreira, Bruno
Villano, Marianna
Majone, Mauro
Valentino, Francesco
author_sort Lorini, Laura
collection PubMed
description Polyhydroxyalkanoates (PHAs) production at pilot scale has been recently investigated and carried out exploiting different process configurations and organic wastes. More in detail, three pilot platforms, in Treviso (North-East of Italy), Carbonera (North-East of Italy) and Lisbon, produced PHAs by open mixed microbial cultures (MMCs) and different organic waste streams: organic fraction of municipal solid waste and sewage sludge (OFMSW-WAS), cellulosic primary sludge (CPS), and fruit waste (FW), respectively. In this context, two stabilization methods have been applied, and compared, for preserving the amount of PHA inside the cells: thermal drying and wet acidification of the biomass at the end of PHA accumulation process. Afterward, polymer has been extracted following an optimized method based on aqueous-phase inorganic reagents. Several PHA samples were then characterized to determine PHA purity, chemical composition, molecular weight, and thermal properties. The polymer contained two types of monomers, namely 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) at a relative percentage of 92.6–79.8 and 7.4–20.2 w/w, respectively, for Treviso and Lisbon plants. On the other hand, an opposite range was found for 3HB and 3HV monomers of PHA from Carbonera, which is 44.0–13.0 and 56.0–87.0 w/w, respectively. PHA extracted from wet-acidified biomass had generally higher viscosity average molecular weights (M(v)) (on average 424.8 ± 20.6 and 224.9 ± 21.9 KDa, respectively, for Treviso and Lisbon) while PHA recovered from thermally stabilized dried biomass had a three-fold lower M(v).
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spelling pubmed-79319942021-03-05 Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes Lorini, Laura Martinelli, Andrea Capuani, Giorgio Frison, Nicola Reis, Maria Sommer Ferreira, Bruno Villano, Marianna Majone, Mauro Valentino, Francesco Front Bioeng Biotechnol Bioengineering and Biotechnology Polyhydroxyalkanoates (PHAs) production at pilot scale has been recently investigated and carried out exploiting different process configurations and organic wastes. More in detail, three pilot platforms, in Treviso (North-East of Italy), Carbonera (North-East of Italy) and Lisbon, produced PHAs by open mixed microbial cultures (MMCs) and different organic waste streams: organic fraction of municipal solid waste and sewage sludge (OFMSW-WAS), cellulosic primary sludge (CPS), and fruit waste (FW), respectively. In this context, two stabilization methods have been applied, and compared, for preserving the amount of PHA inside the cells: thermal drying and wet acidification of the biomass at the end of PHA accumulation process. Afterward, polymer has been extracted following an optimized method based on aqueous-phase inorganic reagents. Several PHA samples were then characterized to determine PHA purity, chemical composition, molecular weight, and thermal properties. The polymer contained two types of monomers, namely 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) at a relative percentage of 92.6–79.8 and 7.4–20.2 w/w, respectively, for Treviso and Lisbon plants. On the other hand, an opposite range was found for 3HB and 3HV monomers of PHA from Carbonera, which is 44.0–13.0 and 56.0–87.0 w/w, respectively. PHA extracted from wet-acidified biomass had generally higher viscosity average molecular weights (M(v)) (on average 424.8 ± 20.6 and 224.9 ± 21.9 KDa, respectively, for Treviso and Lisbon) while PHA recovered from thermally stabilized dried biomass had a three-fold lower M(v). Frontiers Media S.A. 2021-02-18 /pmc/articles/PMC7931994/ /pubmed/33681164 http://dx.doi.org/10.3389/fbioe.2021.628719 Text en Copyright © 2021 Lorini, Martinelli, Capuani, Frison, Reis, Sommer Ferreira, Villano, Majone and Valentino. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Lorini, Laura
Martinelli, Andrea
Capuani, Giorgio
Frison, Nicola
Reis, Maria
Sommer Ferreira, Bruno
Villano, Marianna
Majone, Mauro
Valentino, Francesco
Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes
title Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes
title_full Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes
title_fullStr Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes
title_full_unstemmed Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes
title_short Characterization of Polyhydroxyalkanoates Produced at Pilot Scale From Different Organic Wastes
title_sort characterization of polyhydroxyalkanoates produced at pilot scale from different organic wastes
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931994/
https://www.ncbi.nlm.nih.gov/pubmed/33681164
http://dx.doi.org/10.3389/fbioe.2021.628719
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