Characterization of CRISPR/Cas9 RANKL knockout mesenchymal stem cell clones based on single-cell printing technology and Emulsion Coupling assay as a low-cellularity workflow for single-cell cloning

The homogeneity of the genetically modified single-cells is a necessity for many applications such as cell line development, gene therapy, and tissue engineering and in particular for regenerative medical applications. The lack of tools to effectively isolate and characterize CRISPR/Cas9 engineered...

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Detalles Bibliográficos
Autores principales: Gross, Tobias, Jeney, Csaba, Halm, Darius, Finkenzeller, Günter, Stark, G. Björn, Zengerle, Roland, Koltay, Peter, Zimmermann, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7932140/
https://www.ncbi.nlm.nih.gov/pubmed/33661950
http://dx.doi.org/10.1371/journal.pone.0238330

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7932140/
https://www.ncbi.nlm.nih.gov/pubmed/33661950
http://dx.doi.org/10.1371/journal.pone.0238330

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