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Non-genetic inheritance restraint of cell-to-cell variation
Heterogeneity in physical and functional characteristics of cells (e.g. size, cycle time, growth rate, protein concentration) proliferates within an isogenic population due to stochasticity in intracellular biochemical processes and in the distribution of resources during divisions. Conversely, it i...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7932692/ https://www.ncbi.nlm.nih.gov/pubmed/33523801 http://dx.doi.org/10.7554/eLife.64779 |
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author | Vashistha, Harsh Kohram, Maryam Salman, Hanna |
author_facet | Vashistha, Harsh Kohram, Maryam Salman, Hanna |
author_sort | Vashistha, Harsh |
collection | PubMed |
description | Heterogeneity in physical and functional characteristics of cells (e.g. size, cycle time, growth rate, protein concentration) proliferates within an isogenic population due to stochasticity in intracellular biochemical processes and in the distribution of resources during divisions. Conversely, it is limited in part by the inheritance of cellular components between consecutive generations. Here we introduce a new experimental method for measuring proliferation of heterogeneity in bacterial cell characteristics, based on measuring how two sister cells become different from each other over time. Our measurements provide the inheritance dynamics of different cellular properties, and the ‘inertia’ of cells to maintain these properties along time. We find that inheritance dynamics are property specific and can exhibit long-term memory (∼10 generations) that works to restrain variation among cells. Our results can reveal mechanisms of non-genetic inheritance in bacteria and help understand how cells control their properties and heterogeneity within isogenic cell populations. |
format | Online Article Text |
id | pubmed-7932692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-79326922021-03-08 Non-genetic inheritance restraint of cell-to-cell variation Vashistha, Harsh Kohram, Maryam Salman, Hanna eLife Cell Biology Heterogeneity in physical and functional characteristics of cells (e.g. size, cycle time, growth rate, protein concentration) proliferates within an isogenic population due to stochasticity in intracellular biochemical processes and in the distribution of resources during divisions. Conversely, it is limited in part by the inheritance of cellular components between consecutive generations. Here we introduce a new experimental method for measuring proliferation of heterogeneity in bacterial cell characteristics, based on measuring how two sister cells become different from each other over time. Our measurements provide the inheritance dynamics of different cellular properties, and the ‘inertia’ of cells to maintain these properties along time. We find that inheritance dynamics are property specific and can exhibit long-term memory (∼10 generations) that works to restrain variation among cells. Our results can reveal mechanisms of non-genetic inheritance in bacteria and help understand how cells control their properties and heterogeneity within isogenic cell populations. eLife Sciences Publications, Ltd 2021-02-01 /pmc/articles/PMC7932692/ /pubmed/33523801 http://dx.doi.org/10.7554/eLife.64779 Text en © 2021, Vashistha et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Vashistha, Harsh Kohram, Maryam Salman, Hanna Non-genetic inheritance restraint of cell-to-cell variation |
title | Non-genetic inheritance restraint of cell-to-cell variation |
title_full | Non-genetic inheritance restraint of cell-to-cell variation |
title_fullStr | Non-genetic inheritance restraint of cell-to-cell variation |
title_full_unstemmed | Non-genetic inheritance restraint of cell-to-cell variation |
title_short | Non-genetic inheritance restraint of cell-to-cell variation |
title_sort | non-genetic inheritance restraint of cell-to-cell variation |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7932692/ https://www.ncbi.nlm.nih.gov/pubmed/33523801 http://dx.doi.org/10.7554/eLife.64779 |
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