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Cohesin architecture and clustering in vivo
Cohesin helps mediate sister chromatid cohesion, chromosome condensation, DNA repair, and transcription regulation. We exploited proximity-dependent labeling to define the in vivo interactions of cohesin domains with DNA or with other cohesin domains that lie within the same or in different cohesin...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7932697/ https://www.ncbi.nlm.nih.gov/pubmed/33594972 http://dx.doi.org/10.7554/eLife.62243 |
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author | Xiang, Siheng Koshland, Douglas |
author_facet | Xiang, Siheng Koshland, Douglas |
author_sort | Xiang, Siheng |
collection | PubMed |
description | Cohesin helps mediate sister chromatid cohesion, chromosome condensation, DNA repair, and transcription regulation. We exploited proximity-dependent labeling to define the in vivo interactions of cohesin domains with DNA or with other cohesin domains that lie within the same or in different cohesin complexes. Our results suggest that both cohesin's head and hinge domains are proximal to DNA, and cohesin structure is dynamic with differential folding of its coiled coil regions to generate butterfly confirmations. This method also reveals that cohesins form ordered clusters on and off DNA. The levels of cohesin clusters and their distribution on chromosomes are cell cycle-regulated. Cohesin clustering is likely necessary for cohesion maintenance because clustering and maintenance uniquely require the same subset of cohesin domains and the auxiliary cohesin factor Pds5p. These conclusions provide important new mechanistic and biological insights into the architecture of the cohesin complex, cohesin–cohesin interactions, and cohesin's tethering and loop-extruding activities. |
format | Online Article Text |
id | pubmed-7932697 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-79326972021-03-08 Cohesin architecture and clustering in vivo Xiang, Siheng Koshland, Douglas eLife Chromosomes and Gene Expression Cohesin helps mediate sister chromatid cohesion, chromosome condensation, DNA repair, and transcription regulation. We exploited proximity-dependent labeling to define the in vivo interactions of cohesin domains with DNA or with other cohesin domains that lie within the same or in different cohesin complexes. Our results suggest that both cohesin's head and hinge domains are proximal to DNA, and cohesin structure is dynamic with differential folding of its coiled coil regions to generate butterfly confirmations. This method also reveals that cohesins form ordered clusters on and off DNA. The levels of cohesin clusters and their distribution on chromosomes are cell cycle-regulated. Cohesin clustering is likely necessary for cohesion maintenance because clustering and maintenance uniquely require the same subset of cohesin domains and the auxiliary cohesin factor Pds5p. These conclusions provide important new mechanistic and biological insights into the architecture of the cohesin complex, cohesin–cohesin interactions, and cohesin's tethering and loop-extruding activities. eLife Sciences Publications, Ltd 2021-02-17 /pmc/articles/PMC7932697/ /pubmed/33594972 http://dx.doi.org/10.7554/eLife.62243 Text en © 2021, Xiang and Koshland http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Chromosomes and Gene Expression Xiang, Siheng Koshland, Douglas Cohesin architecture and clustering in vivo |
title | Cohesin architecture and clustering in vivo |
title_full | Cohesin architecture and clustering in vivo |
title_fullStr | Cohesin architecture and clustering in vivo |
title_full_unstemmed | Cohesin architecture and clustering in vivo |
title_short | Cohesin architecture and clustering in vivo |
title_sort | cohesin architecture and clustering in vivo |
topic | Chromosomes and Gene Expression |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7932697/ https://www.ncbi.nlm.nih.gov/pubmed/33594972 http://dx.doi.org/10.7554/eLife.62243 |
work_keys_str_mv | AT xiangsiheng cohesinarchitectureandclusteringinvivo AT koshlanddouglas cohesinarchitectureandclusteringinvivo |