Cargando…

Site-specific N-glycosylation analysis of animal cell culture-derived Zika virus proteins

Here, we present for the first time, a site-specific N-glycosylation analysis of proteins from a Brazilian Zika virus (ZIKV) strain. The virus was propagated with high yield in an embryo-derived stem cell line (EB66, Valneva SE), and concentrated by g-force step-gradient centrifugation. Subsequently...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pralow, Alexander, Nikolay, Alexander, Leon, Arnaud, Genzel, Yvonne, Rapp, Erdmann, Reichl, Udo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933209/ https://www.ncbi.nlm.nih.gov/pubmed/33664361 http://dx.doi.org/10.1038/s41598-021-84682-z

Ejemplares similares

Impact of different influenza cultivation conditions on HA N-Glycosylation
por: Roedig, Jana V, et al.
Publicado: (2011)

Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal cleavage specificity of flavastacin for N-glycosylated asparagine
por: Pralow, Alexander, et al.
Publicado: (2017)

Process intensification of EB66® cell cultivations leads to high-yield yellow fever and Zika virus production
por: Nikolay, Alexander, et al.
Publicado: (2018)

Impact of Host Cell Line Adaptation on Quasispecies Composition and Glycosylation of Influenza A Virus Hemagglutinin
por: Roedig, Jana Verena, et al.
Publicado: (2011)

Site-specific O-Glycosylation Analysis of Human Blood Plasma Proteins
por: Hoffmann, Marcus, et al.
Publicado: (2016)

Cell-free N-glycosylation of peptides using synthetic lipid-linked hybrid and complex N-glycans
por: Wenzel, Lisa, et al.
Publicado: (2023)

MDCK and Vero cells for influenza virus vaccine production: a one-to-one comparison up to lab-scale bioreactor cultivation
por: Genzel, Yvonne, et al.
Publicado: (2010)

Metabolic effects of influenza virus infection in cultured animal cells: Intra- and extracellular metabolite profiling
por: Ritter, Joachim B, et al.
Publicado: (2010)

Cell culture-based production of defective interfering particles for influenza antiviral therapy
por: Wasik, Milena A., et al.
Publicado: (2017)

Bioreactors for high cell density and continuous multi-stage cultivations: options for process intensification in cell culture-based viral vaccine production
por: Tapia, Felipe, et al.
Publicado: (2016)

Tracking changes in adaptation to suspension growth for MDCK cells: cell growth correlates with levels of metabolites, enzymes and proteins
por: Pech, Sabine, et al.
Publicado: (2021)

Application of an Inclined Settler for Cell Culture-Based Influenza A Virus Production in Perfusion Mode
por: Coronel, Juliana, et al.
Publicado: (2020)

Production of Modified Vaccinia Ankara Virus by Intensified Cell Cultures: A Comparison of Platform Technologies for Viral Vector Production
por: Gränicher, Gwendal, et al.
Publicado: (2020)

Flow cytometric monitoring of influenza A virus infection in MDCK cells during vaccine production
por: Schulze-Horsel, Josef, et al.
Publicado: (2008)

Effect of influenza virus infection on key metabolic enzyme activities in MDCK cells
por: Janke, Robert, et al.
Publicado: (2011)

Efficient and stable production of Modified Vaccinia Ankara virus in two-stage semi-continuous and in continuous stirred tank cultivation systems
por: Tapia, Felipe, et al.
Publicado: (2017)

Production of Defective Interfering Particles of Influenza A Virus in Parallel Continuous Cultures at Two Residence Times—Insights From qPCR Measurements and Viral Dynamics Modeling
por: Tapia, Felipe, et al.
Publicado: (2019)

Cell culture–based production of defective interfering influenza A virus particles in perfusion mode using an alternating tangential flow filtration system
por: Hein, Marc D., et al.
Publicado: (2021)

Elements in the Development of a Production Process for Modified Vaccinia Virus Ankara
por: Jordan, Ingo, et al.
Publicado: (2013)

Impact of Influenza A Virus Infection on Growth and Metabolism of Suspension MDCK Cells Using a Dynamic Model
por: Ramos, João Rodrigues Correia, et al.
Publicado: (2022)

Continuous Influenza Virus Production in Cell Culture Shows a Periodic Accumulation of Defective Interfering Particles
por: Frensing, Timo, et al.
Publicado: (2013)

Cell-line screening and process development for a fusogenic oncolytic virus in small-scale suspension cultures
por: Göbel, Sven, et al.
Publicado: (2022)

Reliable N-Glycan Analysis–Removal of Frequently Occurring Oligosaccharide Impurities by Enzymatic Degradation
por: Burock, Robert, et al.
Publicado: (2023)

High titer MVA and influenza A virus production using a hybrid fed-batch/perfusion strategy with an ATF system
por: Vázquez-Ramírez, Daniel, et al.
Publicado: (2019)

High-cell-density cultivations to increase MVA virus production
por: Vázquez-Ramírez, Daniel, et al.
Publicado: (2018)

Production of retroviral vectors in continuous high cell density culture
por: Hein, Marc D., et al.
Publicado: (2023)

Performance of an acoustic settler versus a hollow fiber–based ATF technology for influenza virus production in perfusion
por: Gränicher, Gwendal, et al.
Publicado: (2020)

Continuous influenza virus production in a tubular bioreactor system provides stable titers and avoids the “von Magnus effect”
por: Tapia, Felipe, et al.
Publicado: (2019)

Enzymatic Cascades for Tailored (13)C(6) and (15)N Enriched Human Milk Oligosaccharides
por: Fischöder, Thomas, et al.
Publicado: (2019)

OP7, a novel influenza A virus defective interfering particle: production, purification, and animal experiments demonstrating antiviral potential
por: Hein, Marc D., et al.
Publicado: (2020)

Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particles
por: Hein, Marc D., et al.
Publicado: (2021)

High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cells
por: Wu, Yixiao, et al.
Publicado: (2021)

Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells
por: Lohr, Verena, et al.
Publicado: (2012)

The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis
por: Lohr, Verena, et al.
Publicado: (2014)

N-glycosylation in the Pre-Membrane Protein Is Essential for the Zika Virus Life Cycle
por: Gwon, Yong-Dae, et al.
Publicado: (2020)

Multiscale model of defective interfering particle replication for influenza A virus infection in animal cell culture
por: Rüdiger, Daniel, et al.
Publicado: (2021)

Combining functional metagenomics and glycoanalytics to identify enzymes that facilitate structural characterization of sulfated N-glycans
por: Chuzel, Léa, et al.
Publicado: (2021)

Glycomic Characterization of Induced Pluripotent Stem Cells Derived from a Patient Suffering from Phosphomannomutase 2 Congenital Disorder of Glycosylation (PMM2-CDG)
por: Thiesler, Christina T., et al.
Publicado: (2016)

Site-specific N- and O-glycosylation analysis of atacicept
por: Stavenhagen, Kathrin, et al.
Publicado: (2019)

Mutation of N-glycosylation Sites in Salmonid Alphavirus (SAV) Envelope Proteins Attenuate the Virus in Cell Culture
por: Aksnes, Ida, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_7bpt9g6ikkak9ciccg6hniniuf