Structure-guided selection of puromycin N-acetyltransferase mutants with enhanced selection stringency for deriving mammalian cell lines expressing recombinant proteins

Puromycin and the Streptomyces alboniger-derived puromycin N-acetyltransferase (PAC) enzyme form a commonly used system for selecting stably transfected cultured cells. The crystal structure of PAC has been solved using X-ray crystallography, revealing it to be a member of the GCN5-related N-acetylt...

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Detalles Bibliográficos
Autores principales: Caputo, Alessandro T., Eder, Oliver M., Bereznakova, Hana, Pothuis, Heleen, Ardevol, Albert, Newman, Janet, Nuttall, Stewart, Peat, Thomas S., Adams, Timothy E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933286/
https://www.ncbi.nlm.nih.gov/pubmed/33664348
http://dx.doi.org/10.1038/s41598-021-84551-9

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