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Cytochalasins from Xylaria sp. CFL5, an Endophytic Fungus of Cephalotaxus fortunei

ABSTRACT: Three previously undescribed cytochalasins, named xylariasins A‒C (1‒3), together with six known ones (4‒9) were isolated from Xylaria sp. CFL5, an endophytic fungus of Cephalotaxus fortunei. The chemical structures of all new compounds were elucidated on the basis of extensive spectroscop...

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Autores principales: Ma, Kai-Liang, Dong, Shi-Hui, Li, Hang-Ying, Wei, Wen-Jun, Tu, Yong-Qiang, Gao, Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933320/
https://www.ncbi.nlm.nih.gov/pubmed/33146865
http://dx.doi.org/10.1007/s13659-020-00279-5
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author Ma, Kai-Liang
Dong, Shi-Hui
Li, Hang-Ying
Wei, Wen-Jun
Tu, Yong-Qiang
Gao, Kun
author_facet Ma, Kai-Liang
Dong, Shi-Hui
Li, Hang-Ying
Wei, Wen-Jun
Tu, Yong-Qiang
Gao, Kun
author_sort Ma, Kai-Liang
collection PubMed
description ABSTRACT: Three previously undescribed cytochalasins, named xylariasins A‒C (1‒3), together with six known ones (4‒9) were isolated from Xylaria sp. CFL5, an endophytic fungus of Cephalotaxus fortunei. The chemical structures of all new compounds were elucidated on the basis of extensive spectroscopic data analyses and electronic circular dichroism calculation, as well as optical rotation calculation. Biological activities of compounds 1, 4‒9 were evaluated, including cytotoxic, LAG3/MHC II binding inhibition and LAG3/FGL1 binding inhibition activities. Compounds 6 and 9 possessed cytotoxicity against AGS cells at 5 μM, with inhibition rates of 94% and 64%, respectively. In addition, all tested isolates, except compound 6, exhibited obvious inhibitory activity against the interaction of both LAG3/MHC II and LAG3/FGL1. Compounds 1, 5, 7, and 8 inhibited LAG3/MHC II with IC(50) values ranging from 2.37 to 4.74 μM. Meanwhile, the IC(50) values of compounds 1, 7, and 8 against LAG3/FGL1 were 11.78, 4.39, and 7.45 μM, respectively. GRAPHIC ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13659-020-00279-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-79333202021-03-19 Cytochalasins from Xylaria sp. CFL5, an Endophytic Fungus of Cephalotaxus fortunei Ma, Kai-Liang Dong, Shi-Hui Li, Hang-Ying Wei, Wen-Jun Tu, Yong-Qiang Gao, Kun Nat Prod Bioprospect Original Article ABSTRACT: Three previously undescribed cytochalasins, named xylariasins A‒C (1‒3), together with six known ones (4‒9) were isolated from Xylaria sp. CFL5, an endophytic fungus of Cephalotaxus fortunei. The chemical structures of all new compounds were elucidated on the basis of extensive spectroscopic data analyses and electronic circular dichroism calculation, as well as optical rotation calculation. Biological activities of compounds 1, 4‒9 were evaluated, including cytotoxic, LAG3/MHC II binding inhibition and LAG3/FGL1 binding inhibition activities. Compounds 6 and 9 possessed cytotoxicity against AGS cells at 5 μM, with inhibition rates of 94% and 64%, respectively. In addition, all tested isolates, except compound 6, exhibited obvious inhibitory activity against the interaction of both LAG3/MHC II and LAG3/FGL1. Compounds 1, 5, 7, and 8 inhibited LAG3/MHC II with IC(50) values ranging from 2.37 to 4.74 μM. Meanwhile, the IC(50) values of compounds 1, 7, and 8 against LAG3/FGL1 were 11.78, 4.39, and 7.45 μM, respectively. GRAPHIC ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13659-020-00279-5) contains supplementary material, which is available to authorized users. Springer Singapore 2020-11-04 /pmc/articles/PMC7933320/ /pubmed/33146865 http://dx.doi.org/10.1007/s13659-020-00279-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Ma, Kai-Liang
Dong, Shi-Hui
Li, Hang-Ying
Wei, Wen-Jun
Tu, Yong-Qiang
Gao, Kun
Cytochalasins from Xylaria sp. CFL5, an Endophytic Fungus of Cephalotaxus fortunei
title Cytochalasins from Xylaria sp. CFL5, an Endophytic Fungus of Cephalotaxus fortunei
title_full Cytochalasins from Xylaria sp. CFL5, an Endophytic Fungus of Cephalotaxus fortunei
title_fullStr Cytochalasins from Xylaria sp. CFL5, an Endophytic Fungus of Cephalotaxus fortunei
title_full_unstemmed Cytochalasins from Xylaria sp. CFL5, an Endophytic Fungus of Cephalotaxus fortunei
title_short Cytochalasins from Xylaria sp. CFL5, an Endophytic Fungus of Cephalotaxus fortunei
title_sort cytochalasins from xylaria sp. cfl5, an endophytic fungus of cephalotaxus fortunei
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933320/
https://www.ncbi.nlm.nih.gov/pubmed/33146865
http://dx.doi.org/10.1007/s13659-020-00279-5
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