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Listeriosis Cases and Genetic Diversity of Their L. monocytogenes Isolates in China, 2008–2019

Listeriosis, caused by Listeria monocytogenes, is a severe food-borne infection. The nationwide surveillance in China concerning listeriosis is urgently needed. In the present study, 144 L. monocytogenes isolates were collected from the samples of blood, cerebrospinal fluid (CSF), and fetal membrane...

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Autores principales: Lu, Binghuai, Yang, Junwen, Gao, Chunyan, Li, Dong, Cui, Yanchao, Huang, Lei, Chen, Xingchun, Wang, Duochun, Wang, Aiping, Liu, Yulei, Li, Yi, Zhang, Zhijun, Jiao, Mingyuan, Xu, Heping, Song, Yu, Fu, Baoqing, Xu, Lili, Yang, Qing, Ning, Yongzhong, Wang, Lijun, Bao, Chunmei, Luo, Guolan, Wu, Hua, Yang, Tongshu, Li, Chen, Tang, Manjuan, Wang, Junrui, Guo, Wenchen, Zeng, Ji, Zhong, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933659/
https://www.ncbi.nlm.nih.gov/pubmed/33680989
http://dx.doi.org/10.3389/fcimb.2021.608352
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author Lu, Binghuai
Yang, Junwen
Gao, Chunyan
Li, Dong
Cui, Yanchao
Huang, Lei
Chen, Xingchun
Wang, Duochun
Wang, Aiping
Liu, Yulei
Li, Yi
Zhang, Zhijun
Jiao, Mingyuan
Xu, Heping
Song, Yu
Fu, Baoqing
Xu, Lili
Yang, Qing
Ning, Yongzhong
Wang, Lijun
Bao, Chunmei
Luo, Guolan
Wu, Hua
Yang, Tongshu
Li, Chen
Tang, Manjuan
Wang, Junrui
Guo, Wenchen
Zeng, Ji
Zhong, Wen
author_facet Lu, Binghuai
Yang, Junwen
Gao, Chunyan
Li, Dong
Cui, Yanchao
Huang, Lei
Chen, Xingchun
Wang, Duochun
Wang, Aiping
Liu, Yulei
Li, Yi
Zhang, Zhijun
Jiao, Mingyuan
Xu, Heping
Song, Yu
Fu, Baoqing
Xu, Lili
Yang, Qing
Ning, Yongzhong
Wang, Lijun
Bao, Chunmei
Luo, Guolan
Wu, Hua
Yang, Tongshu
Li, Chen
Tang, Manjuan
Wang, Junrui
Guo, Wenchen
Zeng, Ji
Zhong, Wen
author_sort Lu, Binghuai
collection PubMed
description Listeriosis, caused by Listeria monocytogenes, is a severe food-borne infection. The nationwide surveillance in China concerning listeriosis is urgently needed. In the present study, 144 L. monocytogenes isolates were collected from the samples of blood, cerebrospinal fluid (CSF), and fetal membrane/placenta in China for 12 years from 2008 to 2019. We summarized these listeriosis patients’ demographical and clinical features and outcomes. The susceptibility profile for 12 antibiotics was also determined by the broth microdilution method. Multilocus sequence typing (MLST) and serogroups of these listeria isolates were analyzed to designate epidemiological types. We enrolled 144 cases from 29 healthcare centers, including 96 maternal-neonatal infections, 33 cases of bacteremia, 13 cases of neurolisteriosis, and two cutaneous listeriosis. There were 31 (59.6%) fetal loss in 52 pregnant women and four (9.8%) neonatal death in 41 newborns. Among the 48 nonmaternal-neonatal cases, 12.5% (6/48) died, 41.7% (20/48) were female, and 64.6% (31/48) occurred in those with significant comorbidities. By MLST, the strains were distinguished into 23 individual sequence types (STs). The most prevalent ST was ST87 (49 isolates, 34.0%), followed by ST1 (18, 12.5%), ST8 (10, 6.9%), ST619 (9, 6.3%), ST7 (7, 4.9%) and ST3 (7, 4.9%). Furthermore, all L. monocytogenes isolates were uniformly susceptible to penicillin, ampicillin, and meropenem. In summary, our study highlights a high genotypic diversity of L. monocytogenes strains causing clinical listeriosis in China. Furthermore, a high prevalence of ST87 and ST1 in the listeriosis should be noted.
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spelling pubmed-79336592021-03-06 Listeriosis Cases and Genetic Diversity of Their L. monocytogenes Isolates in China, 2008–2019 Lu, Binghuai Yang, Junwen Gao, Chunyan Li, Dong Cui, Yanchao Huang, Lei Chen, Xingchun Wang, Duochun Wang, Aiping Liu, Yulei Li, Yi Zhang, Zhijun Jiao, Mingyuan Xu, Heping Song, Yu Fu, Baoqing Xu, Lili Yang, Qing Ning, Yongzhong Wang, Lijun Bao, Chunmei Luo, Guolan Wu, Hua Yang, Tongshu Li, Chen Tang, Manjuan Wang, Junrui Guo, Wenchen Zeng, Ji Zhong, Wen Front Cell Infect Microbiol Cellular and Infection Microbiology Listeriosis, caused by Listeria monocytogenes, is a severe food-borne infection. The nationwide surveillance in China concerning listeriosis is urgently needed. In the present study, 144 L. monocytogenes isolates were collected from the samples of blood, cerebrospinal fluid (CSF), and fetal membrane/placenta in China for 12 years from 2008 to 2019. We summarized these listeriosis patients’ demographical and clinical features and outcomes. The susceptibility profile for 12 antibiotics was also determined by the broth microdilution method. Multilocus sequence typing (MLST) and serogroups of these listeria isolates were analyzed to designate epidemiological types. We enrolled 144 cases from 29 healthcare centers, including 96 maternal-neonatal infections, 33 cases of bacteremia, 13 cases of neurolisteriosis, and two cutaneous listeriosis. There were 31 (59.6%) fetal loss in 52 pregnant women and four (9.8%) neonatal death in 41 newborns. Among the 48 nonmaternal-neonatal cases, 12.5% (6/48) died, 41.7% (20/48) were female, and 64.6% (31/48) occurred in those with significant comorbidities. By MLST, the strains were distinguished into 23 individual sequence types (STs). The most prevalent ST was ST87 (49 isolates, 34.0%), followed by ST1 (18, 12.5%), ST8 (10, 6.9%), ST619 (9, 6.3%), ST7 (7, 4.9%) and ST3 (7, 4.9%). Furthermore, all L. monocytogenes isolates were uniformly susceptible to penicillin, ampicillin, and meropenem. In summary, our study highlights a high genotypic diversity of L. monocytogenes strains causing clinical listeriosis in China. Furthermore, a high prevalence of ST87 and ST1 in the listeriosis should be noted. Frontiers Media S.A. 2021-02-19 /pmc/articles/PMC7933659/ /pubmed/33680989 http://dx.doi.org/10.3389/fcimb.2021.608352 Text en Copyright © 2021 Lu, Yang, Gao, Li, Cui, Huang, Chen, Wang, Wang, Liu, Li, Zhang, Jiao, Xu, Song, Fu, Xu, Yang, Ning, Wang, Bao, Luo, Wu, Yang, Li, Tang, Wang, Guo, Zeng and Zhong http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Lu, Binghuai
Yang, Junwen
Gao, Chunyan
Li, Dong
Cui, Yanchao
Huang, Lei
Chen, Xingchun
Wang, Duochun
Wang, Aiping
Liu, Yulei
Li, Yi
Zhang, Zhijun
Jiao, Mingyuan
Xu, Heping
Song, Yu
Fu, Baoqing
Xu, Lili
Yang, Qing
Ning, Yongzhong
Wang, Lijun
Bao, Chunmei
Luo, Guolan
Wu, Hua
Yang, Tongshu
Li, Chen
Tang, Manjuan
Wang, Junrui
Guo, Wenchen
Zeng, Ji
Zhong, Wen
Listeriosis Cases and Genetic Diversity of Their L. monocytogenes Isolates in China, 2008–2019
title Listeriosis Cases and Genetic Diversity of Their L. monocytogenes Isolates in China, 2008–2019
title_full Listeriosis Cases and Genetic Diversity of Their L. monocytogenes Isolates in China, 2008–2019
title_fullStr Listeriosis Cases and Genetic Diversity of Their L. monocytogenes Isolates in China, 2008–2019
title_full_unstemmed Listeriosis Cases and Genetic Diversity of Their L. monocytogenes Isolates in China, 2008–2019
title_short Listeriosis Cases and Genetic Diversity of Their L. monocytogenes Isolates in China, 2008–2019
title_sort listeriosis cases and genetic diversity of their l. monocytogenes isolates in china, 2008–2019
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933659/
https://www.ncbi.nlm.nih.gov/pubmed/33680989
http://dx.doi.org/10.3389/fcimb.2021.608352
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