In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation

Little is known about the emergence of blood progenitors during human embryogenesis due to ethical reasons and restricted embryo access. The use of human embryonic stem cells (hESCs) as a model system offers unique opportunities to dissect human blood cell formation. Here, we describe a protocol all...

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Autores principales: Garcia-Alegria, Eva, Potts, Bethany, Menegatti, Sara, Kouskoff, Valerie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933812/
https://www.ncbi.nlm.nih.gov/pubmed/33718891
http://dx.doi.org/10.1016/j.xpro.2021.100367
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author Garcia-Alegria, Eva
Potts, Bethany
Menegatti, Sara
Kouskoff, Valerie
author_facet Garcia-Alegria, Eva
Potts, Bethany
Menegatti, Sara
Kouskoff, Valerie
author_sort Garcia-Alegria, Eva
collection PubMed
description Little is known about the emergence of blood progenitors during human embryogenesis due to ethical reasons and restricted embryo access. The use of human embryonic stem cells (hESCs) as a model system offers unique opportunities to dissect human blood cell formation. Here, we describe a protocol allowing the differentiation of hESCs via embryoid bodies toward hemogenic endothelium and its subsequent differentiation to blood progenitors. This protocol relies on the formation of embryoid bodies, which is tricky if not carefully performed. For complete details on the use and execution of this protocol, please refer to Garcia-Alegria et al. (2018).
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spelling pubmed-79338122021-03-12 In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation Garcia-Alegria, Eva Potts, Bethany Menegatti, Sara Kouskoff, Valerie STAR Protoc Protocol Little is known about the emergence of blood progenitors during human embryogenesis due to ethical reasons and restricted embryo access. The use of human embryonic stem cells (hESCs) as a model system offers unique opportunities to dissect human blood cell formation. Here, we describe a protocol allowing the differentiation of hESCs via embryoid bodies toward hemogenic endothelium and its subsequent differentiation to blood progenitors. This protocol relies on the formation of embryoid bodies, which is tricky if not carefully performed. For complete details on the use and execution of this protocol, please refer to Garcia-Alegria et al. (2018). Elsevier 2021-03-02 /pmc/articles/PMC7933812/ /pubmed/33718891 http://dx.doi.org/10.1016/j.xpro.2021.100367 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Garcia-Alegria, Eva
Potts, Bethany
Menegatti, Sara
Kouskoff, Valerie
In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation
title In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation
title_full In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation
title_fullStr In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation
title_full_unstemmed In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation
title_short In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation
title_sort in vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933812/
https://www.ncbi.nlm.nih.gov/pubmed/33718891
http://dx.doi.org/10.1016/j.xpro.2021.100367
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