Cargando…

The IL‐27/IL‐27R axis is altered in CD4(+) and CD8(+) T lymphocytes from multiple sclerosis patients

OBJECTIVES: Pro‐ and anti‐inflammatory properties have been attributed to interleukin‐27 (IL‐27). Nevertheless, the impact of this cytokine on chronic inflammatory diseases such as multiple sclerosis (MS) remains ill‐defined. We investigated the biology of IL‐27 and its specific receptor IL‐27Rα in...

Descripción completa

Detalles Bibliográficos
Autores principales: Clénet, Marie‐Laure, Laurent, Cyril, Lemaitre, Florent, Farzam‐kia, Negar, Tastet, Olivier, Devergne, Odile, Lahav, Boaz, Girard, Marc, Duquette, Pierre, Prat, Alexandre, Larochelle, Catherine, Arbour, Nathalie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934284/
https://www.ncbi.nlm.nih.gov/pubmed/33728050
http://dx.doi.org/10.1002/cti2.1262
_version_ 1783660791872880640
author Clénet, Marie‐Laure
Laurent, Cyril
Lemaitre, Florent
Farzam‐kia, Negar
Tastet, Olivier
Devergne, Odile
Lahav, Boaz
Girard, Marc
Duquette, Pierre
Prat, Alexandre
Larochelle, Catherine
Arbour, Nathalie
author_facet Clénet, Marie‐Laure
Laurent, Cyril
Lemaitre, Florent
Farzam‐kia, Negar
Tastet, Olivier
Devergne, Odile
Lahav, Boaz
Girard, Marc
Duquette, Pierre
Prat, Alexandre
Larochelle, Catherine
Arbour, Nathalie
author_sort Clénet, Marie‐Laure
collection PubMed
description OBJECTIVES: Pro‐ and anti‐inflammatory properties have been attributed to interleukin‐27 (IL‐27). Nevertheless, the impact of this cytokine on chronic inflammatory diseases such as multiple sclerosis (MS) remains ill‐defined. We investigated the biology of IL‐27 and its specific receptor IL‐27Rα in MS patients. METHODS: Levels of IL‐27 and its natural antagonist (IL‐27‐Rα) were measured by ELISA in biological fluids. CD4(+) and CD8(+) T lymphocytes were isolated from untreated relapsing–remitting MS patients and healthy donors. Transcriptome‐wide analysis compared T‐cell subsets stimulated or not with IL‐27. Expression of the IL‐27Rα, key immune factors, STAT phosphorylation and cytokine production was assessed by flow cytometry. RESULTS: We observed elevated levels of IL‐27 in the serum and cerebrospinal fluid of MS patients compared with controls. Moreover, we show that specific IL‐27‐mediated effects on T lymphocytes are reduced in MS patients including the induction of PD‐L1. IL‐27‐triggered STAT3 signalling pathway is enhanced in CD4(+) and CD8(+) T lymphocytes from MS patients. Elevated IL‐27Rα levels in serum from MS patients are sufficient to impair the capacity of IL‐27 to act on immune cells. We demonstrate that shedding of IL‐27Rα by activated CD4(+) T lymphocytes from MS patients contributes to the increased IL‐27Rα peripheral levels and consequently can dampen the IL‐27 responsiveness. CONCLUSION: Our work identifies several mechanisms that are altered in the IL‐27/IL‐27R axis in MS patients, especially in T lymphocytes. Our results underline the importance of characterising the biology of cytokines in human patients prior to design new therapeutics.
format Online
Article
Text
id pubmed-7934284
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-79342842021-03-15 The IL‐27/IL‐27R axis is altered in CD4(+) and CD8(+) T lymphocytes from multiple sclerosis patients Clénet, Marie‐Laure Laurent, Cyril Lemaitre, Florent Farzam‐kia, Negar Tastet, Olivier Devergne, Odile Lahav, Boaz Girard, Marc Duquette, Pierre Prat, Alexandre Larochelle, Catherine Arbour, Nathalie Clin Transl Immunology Original Articles OBJECTIVES: Pro‐ and anti‐inflammatory properties have been attributed to interleukin‐27 (IL‐27). Nevertheless, the impact of this cytokine on chronic inflammatory diseases such as multiple sclerosis (MS) remains ill‐defined. We investigated the biology of IL‐27 and its specific receptor IL‐27Rα in MS patients. METHODS: Levels of IL‐27 and its natural antagonist (IL‐27‐Rα) were measured by ELISA in biological fluids. CD4(+) and CD8(+) T lymphocytes were isolated from untreated relapsing–remitting MS patients and healthy donors. Transcriptome‐wide analysis compared T‐cell subsets stimulated or not with IL‐27. Expression of the IL‐27Rα, key immune factors, STAT phosphorylation and cytokine production was assessed by flow cytometry. RESULTS: We observed elevated levels of IL‐27 in the serum and cerebrospinal fluid of MS patients compared with controls. Moreover, we show that specific IL‐27‐mediated effects on T lymphocytes are reduced in MS patients including the induction of PD‐L1. IL‐27‐triggered STAT3 signalling pathway is enhanced in CD4(+) and CD8(+) T lymphocytes from MS patients. Elevated IL‐27Rα levels in serum from MS patients are sufficient to impair the capacity of IL‐27 to act on immune cells. We demonstrate that shedding of IL‐27Rα by activated CD4(+) T lymphocytes from MS patients contributes to the increased IL‐27Rα peripheral levels and consequently can dampen the IL‐27 responsiveness. CONCLUSION: Our work identifies several mechanisms that are altered in the IL‐27/IL‐27R axis in MS patients, especially in T lymphocytes. Our results underline the importance of characterising the biology of cytokines in human patients prior to design new therapeutics. John Wiley and Sons Inc. 2021-03-05 /pmc/articles/PMC7934284/ /pubmed/33728050 http://dx.doi.org/10.1002/cti2.1262 Text en © 2021 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Clénet, Marie‐Laure
Laurent, Cyril
Lemaitre, Florent
Farzam‐kia, Negar
Tastet, Olivier
Devergne, Odile
Lahav, Boaz
Girard, Marc
Duquette, Pierre
Prat, Alexandre
Larochelle, Catherine
Arbour, Nathalie
The IL‐27/IL‐27R axis is altered in CD4(+) and CD8(+) T lymphocytes from multiple sclerosis patients
title The IL‐27/IL‐27R axis is altered in CD4(+) and CD8(+) T lymphocytes from multiple sclerosis patients
title_full The IL‐27/IL‐27R axis is altered in CD4(+) and CD8(+) T lymphocytes from multiple sclerosis patients
title_fullStr The IL‐27/IL‐27R axis is altered in CD4(+) and CD8(+) T lymphocytes from multiple sclerosis patients
title_full_unstemmed The IL‐27/IL‐27R axis is altered in CD4(+) and CD8(+) T lymphocytes from multiple sclerosis patients
title_short The IL‐27/IL‐27R axis is altered in CD4(+) and CD8(+) T lymphocytes from multiple sclerosis patients
title_sort il‐27/il‐27r axis is altered in cd4(+) and cd8(+) t lymphocytes from multiple sclerosis patients
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934284/
https://www.ncbi.nlm.nih.gov/pubmed/33728050
http://dx.doi.org/10.1002/cti2.1262
work_keys_str_mv AT clenetmarielaure theil27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT laurentcyril theil27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT lemaitreflorent theil27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT farzamkianegar theil27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT tastetolivier theil27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT devergneodile theil27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT lahavboaz theil27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT girardmarc theil27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT duquettepierre theil27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT pratalexandre theil27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT larochellecatherine theil27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT arbournathalie theil27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT clenetmarielaure il27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT laurentcyril il27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT lemaitreflorent il27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT farzamkianegar il27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT tastetolivier il27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT devergneodile il27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT lahavboaz il27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT girardmarc il27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT duquettepierre il27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT pratalexandre il27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT larochellecatherine il27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients
AT arbournathalie il27il27raxisisalteredincd4andcd8tlymphocytesfrommultiplesclerosispatients