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The intestinal microbial composition in Greylag geese differs with steatosis induction mode: spontaneous or induced by overfeeding

BACKGROUND: Relationships between microbial composition and steatosis are being extensively studied in mammals, and causal relations have been evidenced. In migratory birds the liver can transiently store lipids during pre-migratory and migratory phases, but little is known about the implications of...

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Autores principales: Knudsen, Christelle, Arroyo, Julien, Even, Maxime, Cauquil, Laurent, Pascal, Géraldine, Fernandez, Xavier, Lavigne, Franck, Davail, Stéphane, Combes, Sylvie, Ricaud, Karine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934468/
https://www.ncbi.nlm.nih.gov/pubmed/33499980
http://dx.doi.org/10.1186/s42523-020-00067-z
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author Knudsen, Christelle
Arroyo, Julien
Even, Maxime
Cauquil, Laurent
Pascal, Géraldine
Fernandez, Xavier
Lavigne, Franck
Davail, Stéphane
Combes, Sylvie
Ricaud, Karine
author_facet Knudsen, Christelle
Arroyo, Julien
Even, Maxime
Cauquil, Laurent
Pascal, Géraldine
Fernandez, Xavier
Lavigne, Franck
Davail, Stéphane
Combes, Sylvie
Ricaud, Karine
author_sort Knudsen, Christelle
collection PubMed
description BACKGROUND: Relationships between microbial composition and steatosis are being extensively studied in mammals, and causal relations have been evidenced. In migratory birds the liver can transiently store lipids during pre-migratory and migratory phases, but little is known about the implications of the digestive microbiota in those mechanisms. The Landaise greylag goose (Anser anser) is a good model to study steatosis in migratory birds as it is domesticated, but is still, from a genetic point of view, close to its wild migratory ancestor. It also has a great ingestion capacity and a good predisposition for hepatic steatosis, whether spontaneous or induced by conventional overfeeding. The conventional (overfeeding) and alternative (spontaneous steatosis induction) systems differ considerably in duration and feed intake level and previous studies have shown that aptitudes to spontaneous steatosis are very variable. The present study thus aimed to address two issues: (i) evaluate whether microbial composition differs with steatosis-inducing mode; (ii) elucidate whether a digestive microbial signature could be associated with variable aptitudes to spontaneous liver steatosis. RESULTS: Performances, biochemical composition of the livers and microbiota differed considerably in response to steatosis stimulation. We namely identified the genus Romboutsia to be overrepresented in birds developing a spontaneous steatosis in comparison to those submitted to conventional overfeeding while the genera Ralstonia, Variovorax and Sphingomonas were underrepresented only in birds that did not develop a spontaneous steatosis compared to conventionally overfed ones, birds developing a spontaneous steatosis having intermediate values. Secondly, no overall differences in microbial composition were evidenced in association with variable aptitudes to spontaneous steatosis, although one OTU, belonging to the Lactobacillus genus, was overrepresented in birds having developed a spontaneous steatosis compared to those that had not. CONCLUSIONS: Our study is the first to evaluate the intestinal microbial composition in association with steatosis, whether spontaneous or induced by overfeeding, in geese. Steatosis induction modes were associated with distinct digestive microbial compositions. However, unlike what can be observed in mammals, no clear microbial signature associated with spontaneous steatosis level was identified.
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spelling pubmed-79344682021-03-09 The intestinal microbial composition in Greylag geese differs with steatosis induction mode: spontaneous or induced by overfeeding Knudsen, Christelle Arroyo, Julien Even, Maxime Cauquil, Laurent Pascal, Géraldine Fernandez, Xavier Lavigne, Franck Davail, Stéphane Combes, Sylvie Ricaud, Karine Anim Microbiome Research Article BACKGROUND: Relationships between microbial composition and steatosis are being extensively studied in mammals, and causal relations have been evidenced. In migratory birds the liver can transiently store lipids during pre-migratory and migratory phases, but little is known about the implications of the digestive microbiota in those mechanisms. The Landaise greylag goose (Anser anser) is a good model to study steatosis in migratory birds as it is domesticated, but is still, from a genetic point of view, close to its wild migratory ancestor. It also has a great ingestion capacity and a good predisposition for hepatic steatosis, whether spontaneous or induced by conventional overfeeding. The conventional (overfeeding) and alternative (spontaneous steatosis induction) systems differ considerably in duration and feed intake level and previous studies have shown that aptitudes to spontaneous steatosis are very variable. The present study thus aimed to address two issues: (i) evaluate whether microbial composition differs with steatosis-inducing mode; (ii) elucidate whether a digestive microbial signature could be associated with variable aptitudes to spontaneous liver steatosis. RESULTS: Performances, biochemical composition of the livers and microbiota differed considerably in response to steatosis stimulation. We namely identified the genus Romboutsia to be overrepresented in birds developing a spontaneous steatosis in comparison to those submitted to conventional overfeeding while the genera Ralstonia, Variovorax and Sphingomonas were underrepresented only in birds that did not develop a spontaneous steatosis compared to conventionally overfed ones, birds developing a spontaneous steatosis having intermediate values. Secondly, no overall differences in microbial composition were evidenced in association with variable aptitudes to spontaneous steatosis, although one OTU, belonging to the Lactobacillus genus, was overrepresented in birds having developed a spontaneous steatosis compared to those that had not. CONCLUSIONS: Our study is the first to evaluate the intestinal microbial composition in association with steatosis, whether spontaneous or induced by overfeeding, in geese. Steatosis induction modes were associated with distinct digestive microbial compositions. However, unlike what can be observed in mammals, no clear microbial signature associated with spontaneous steatosis level was identified. BioMed Central 2021-01-06 /pmc/articles/PMC7934468/ /pubmed/33499980 http://dx.doi.org/10.1186/s42523-020-00067-z Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Knudsen, Christelle
Arroyo, Julien
Even, Maxime
Cauquil, Laurent
Pascal, Géraldine
Fernandez, Xavier
Lavigne, Franck
Davail, Stéphane
Combes, Sylvie
Ricaud, Karine
The intestinal microbial composition in Greylag geese differs with steatosis induction mode: spontaneous or induced by overfeeding
title The intestinal microbial composition in Greylag geese differs with steatosis induction mode: spontaneous or induced by overfeeding
title_full The intestinal microbial composition in Greylag geese differs with steatosis induction mode: spontaneous or induced by overfeeding
title_fullStr The intestinal microbial composition in Greylag geese differs with steatosis induction mode: spontaneous or induced by overfeeding
title_full_unstemmed The intestinal microbial composition in Greylag geese differs with steatosis induction mode: spontaneous or induced by overfeeding
title_short The intestinal microbial composition in Greylag geese differs with steatosis induction mode: spontaneous or induced by overfeeding
title_sort intestinal microbial composition in greylag geese differs with steatosis induction mode: spontaneous or induced by overfeeding
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934468/
https://www.ncbi.nlm.nih.gov/pubmed/33499980
http://dx.doi.org/10.1186/s42523-020-00067-z
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