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Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils

Neutrophils play a key role in the human immune response to Staphylococcus aureus infections. These professional phagocytes rapidly migrate to the site of infection to engulf bacteria and destroy them via specialized intracellular killing mechanisms. Here we describe a robust and relatively high-thr...

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Autores principales: Boero, Elena, Brinkman, Iris, Juliet, Thessely, van Yperen, Eline, van Strijp, Jos A. G., Rooijakkers, Suzan H. M., van Kessel, Kok P. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934835/
https://www.ncbi.nlm.nih.gov/pubmed/33679791
http://dx.doi.org/10.3389/fimmu.2021.635825
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author Boero, Elena
Brinkman, Iris
Juliet, Thessely
van Yperen, Eline
van Strijp, Jos A. G.
Rooijakkers, Suzan H. M.
van Kessel, Kok P. M.
author_facet Boero, Elena
Brinkman, Iris
Juliet, Thessely
van Yperen, Eline
van Strijp, Jos A. G.
Rooijakkers, Suzan H. M.
van Kessel, Kok P. M.
author_sort Boero, Elena
collection PubMed
description Neutrophils play a key role in the human immune response to Staphylococcus aureus infections. These professional phagocytes rapidly migrate to the site of infection to engulf bacteria and destroy them via specialized intracellular killing mechanisms. Here we describe a robust and relatively high-throughput flow cytometry assay to quantify phagocytosis of S. aureus by human neutrophils. We show that effective phagocytic uptake of S. aureus is greatly enhanced by opsonization, i.e. the tagging of microbial surfaces with plasma-derived host proteins like antibodies and complement. Our rapid assay to monitor phagocytosis can be used to study neutrophil deficiencies and bacterial evasion, but also provides a powerful tool to assess the opsonic capacity of antibodies, either in the context of natural immune responses or immune therapies.
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spelling pubmed-79348352021-03-06 Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils Boero, Elena Brinkman, Iris Juliet, Thessely van Yperen, Eline van Strijp, Jos A. G. Rooijakkers, Suzan H. M. van Kessel, Kok P. M. Front Immunol Immunology Neutrophils play a key role in the human immune response to Staphylococcus aureus infections. These professional phagocytes rapidly migrate to the site of infection to engulf bacteria and destroy them via specialized intracellular killing mechanisms. Here we describe a robust and relatively high-throughput flow cytometry assay to quantify phagocytosis of S. aureus by human neutrophils. We show that effective phagocytic uptake of S. aureus is greatly enhanced by opsonization, i.e. the tagging of microbial surfaces with plasma-derived host proteins like antibodies and complement. Our rapid assay to monitor phagocytosis can be used to study neutrophil deficiencies and bacterial evasion, but also provides a powerful tool to assess the opsonic capacity of antibodies, either in the context of natural immune responses or immune therapies. Frontiers Media S.A. 2021-02-19 /pmc/articles/PMC7934835/ /pubmed/33679791 http://dx.doi.org/10.3389/fimmu.2021.635825 Text en Copyright © 2021 Boero, Brinkman, Juliet, van Yperen, van Strijp, Rooijakkers and van Kessel http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Boero, Elena
Brinkman, Iris
Juliet, Thessely
van Yperen, Eline
van Strijp, Jos A. G.
Rooijakkers, Suzan H. M.
van Kessel, Kok P. M.
Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils
title Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils
title_full Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils
title_fullStr Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils
title_full_unstemmed Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils
title_short Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils
title_sort use of flow cytometry to evaluate phagocytosis of staphylococcus aureus by human neutrophils
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934835/
https://www.ncbi.nlm.nih.gov/pubmed/33679791
http://dx.doi.org/10.3389/fimmu.2021.635825
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