Cargando…
Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils
Neutrophils play a key role in the human immune response to Staphylococcus aureus infections. These professional phagocytes rapidly migrate to the site of infection to engulf bacteria and destroy them via specialized intracellular killing mechanisms. Here we describe a robust and relatively high-thr...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934835/ https://www.ncbi.nlm.nih.gov/pubmed/33679791 http://dx.doi.org/10.3389/fimmu.2021.635825 |
_version_ | 1783660892858089472 |
---|---|
author | Boero, Elena Brinkman, Iris Juliet, Thessely van Yperen, Eline van Strijp, Jos A. G. Rooijakkers, Suzan H. M. van Kessel, Kok P. M. |
author_facet | Boero, Elena Brinkman, Iris Juliet, Thessely van Yperen, Eline van Strijp, Jos A. G. Rooijakkers, Suzan H. M. van Kessel, Kok P. M. |
author_sort | Boero, Elena |
collection | PubMed |
description | Neutrophils play a key role in the human immune response to Staphylococcus aureus infections. These professional phagocytes rapidly migrate to the site of infection to engulf bacteria and destroy them via specialized intracellular killing mechanisms. Here we describe a robust and relatively high-throughput flow cytometry assay to quantify phagocytosis of S. aureus by human neutrophils. We show that effective phagocytic uptake of S. aureus is greatly enhanced by opsonization, i.e. the tagging of microbial surfaces with plasma-derived host proteins like antibodies and complement. Our rapid assay to monitor phagocytosis can be used to study neutrophil deficiencies and bacterial evasion, but also provides a powerful tool to assess the opsonic capacity of antibodies, either in the context of natural immune responses or immune therapies. |
format | Online Article Text |
id | pubmed-7934835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79348352021-03-06 Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils Boero, Elena Brinkman, Iris Juliet, Thessely van Yperen, Eline van Strijp, Jos A. G. Rooijakkers, Suzan H. M. van Kessel, Kok P. M. Front Immunol Immunology Neutrophils play a key role in the human immune response to Staphylococcus aureus infections. These professional phagocytes rapidly migrate to the site of infection to engulf bacteria and destroy them via specialized intracellular killing mechanisms. Here we describe a robust and relatively high-throughput flow cytometry assay to quantify phagocytosis of S. aureus by human neutrophils. We show that effective phagocytic uptake of S. aureus is greatly enhanced by opsonization, i.e. the tagging of microbial surfaces with plasma-derived host proteins like antibodies and complement. Our rapid assay to monitor phagocytosis can be used to study neutrophil deficiencies and bacterial evasion, but also provides a powerful tool to assess the opsonic capacity of antibodies, either in the context of natural immune responses or immune therapies. Frontiers Media S.A. 2021-02-19 /pmc/articles/PMC7934835/ /pubmed/33679791 http://dx.doi.org/10.3389/fimmu.2021.635825 Text en Copyright © 2021 Boero, Brinkman, Juliet, van Yperen, van Strijp, Rooijakkers and van Kessel http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Boero, Elena Brinkman, Iris Juliet, Thessely van Yperen, Eline van Strijp, Jos A. G. Rooijakkers, Suzan H. M. van Kessel, Kok P. M. Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils |
title | Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils |
title_full | Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils |
title_fullStr | Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils |
title_full_unstemmed | Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils |
title_short | Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils |
title_sort | use of flow cytometry to evaluate phagocytosis of staphylococcus aureus by human neutrophils |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934835/ https://www.ncbi.nlm.nih.gov/pubmed/33679791 http://dx.doi.org/10.3389/fimmu.2021.635825 |
work_keys_str_mv | AT boeroelena useofflowcytometrytoevaluatephagocytosisofstaphylococcusaureusbyhumanneutrophils AT brinkmaniris useofflowcytometrytoevaluatephagocytosisofstaphylococcusaureusbyhumanneutrophils AT julietthessely useofflowcytometrytoevaluatephagocytosisofstaphylococcusaureusbyhumanneutrophils AT vanypereneline useofflowcytometrytoevaluatephagocytosisofstaphylococcusaureusbyhumanneutrophils AT vanstrijpjosag useofflowcytometrytoevaluatephagocytosisofstaphylococcusaureusbyhumanneutrophils AT rooijakkerssuzanhm useofflowcytometrytoevaluatephagocytosisofstaphylococcusaureusbyhumanneutrophils AT vankesselkokpm useofflowcytometrytoevaluatephagocytosisofstaphylococcusaureusbyhumanneutrophils |