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Succinylation Regulators Promote Clear Cell Renal Cell Carcinoma by Immune Regulation and RNA N6-Methyladenosine Methylation

Succinylation is a newly discovered and multienzyme-regulated post-translational modification (PTM) that is associated with the initiation and progression of cancer. Currently, no systematic analyses on the role of succinylation regulators in tumors have been reported. In this study, we performed a...

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Autores principales: Lu, Wenqing, Che, Xiaofang, Qu, Xiujuan, Zheng, Chunlei, Yang, Xianghong, Bao, Bowen, Li, Zhi, Wang, Duo, Jin, Yue, Wang, Yizhe, Xiao, Jiawen, Qi, Jianfei, Liu, Yunpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935513/
https://www.ncbi.nlm.nih.gov/pubmed/33681201
http://dx.doi.org/10.3389/fcell.2021.622198
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author Lu, Wenqing
Che, Xiaofang
Qu, Xiujuan
Zheng, Chunlei
Yang, Xianghong
Bao, Bowen
Li, Zhi
Wang, Duo
Jin, Yue
Wang, Yizhe
Xiao, Jiawen
Qi, Jianfei
Liu, Yunpeng
author_facet Lu, Wenqing
Che, Xiaofang
Qu, Xiujuan
Zheng, Chunlei
Yang, Xianghong
Bao, Bowen
Li, Zhi
Wang, Duo
Jin, Yue
Wang, Yizhe
Xiao, Jiawen
Qi, Jianfei
Liu, Yunpeng
author_sort Lu, Wenqing
collection PubMed
description Succinylation is a newly discovered and multienzyme-regulated post-translational modification (PTM) that is associated with the initiation and progression of cancer. Currently, no systematic analyses on the role of succinylation regulators in tumors have been reported. In this study, we performed a comprehensive pan-cancer analysis on four well-known succinylation regulators (CPT1A, KAT2A, SIRT5, and SIRT7). We found that these regulators played specific and critical roles in the prognosis of clear cell renal cell carcinoma (ccRCC). We constructed a risk score (RS) based on two independent prognostic prediction factors, CPT1A and KAT2A, and subsequently developed a nomogram model containing the RS, which showed good accuracy in the prediction of overall survival (OS) in ccRCC patients. Furthermore, we used the similar expression pattern of four succinylation regulators according to consensus clustering analysis to divide the patients into three clusters that exhibited prominently different OS as well as clinicopathological characteristics. Differently expressed genes (DEGs) and pathway enrichment analyses of three clusters indicated that succinylation regulators might promote malignant progression of ccRCC by regulating the infiltration of immune cells and RNA N6-methyladenosine (m6A) methylation. Importantly, our data suggest that CPT1A and SIRT5 might up-regulate and down-regulate the expression of LRPPRC and EIF3B, respectively. Our study systematically analyzed the prognostic predictive values of four succinylation regulators and revealed their potential mechanisms in ccRCC aggressiveness. These data provide new insight into the understanding of succinylation modification and present clinical evidence for its role in ccRCC treatments.
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spelling pubmed-79355132021-03-06 Succinylation Regulators Promote Clear Cell Renal Cell Carcinoma by Immune Regulation and RNA N6-Methyladenosine Methylation Lu, Wenqing Che, Xiaofang Qu, Xiujuan Zheng, Chunlei Yang, Xianghong Bao, Bowen Li, Zhi Wang, Duo Jin, Yue Wang, Yizhe Xiao, Jiawen Qi, Jianfei Liu, Yunpeng Front Cell Dev Biol Cell and Developmental Biology Succinylation is a newly discovered and multienzyme-regulated post-translational modification (PTM) that is associated with the initiation and progression of cancer. Currently, no systematic analyses on the role of succinylation regulators in tumors have been reported. In this study, we performed a comprehensive pan-cancer analysis on four well-known succinylation regulators (CPT1A, KAT2A, SIRT5, and SIRT7). We found that these regulators played specific and critical roles in the prognosis of clear cell renal cell carcinoma (ccRCC). We constructed a risk score (RS) based on two independent prognostic prediction factors, CPT1A and KAT2A, and subsequently developed a nomogram model containing the RS, which showed good accuracy in the prediction of overall survival (OS) in ccRCC patients. Furthermore, we used the similar expression pattern of four succinylation regulators according to consensus clustering analysis to divide the patients into three clusters that exhibited prominently different OS as well as clinicopathological characteristics. Differently expressed genes (DEGs) and pathway enrichment analyses of three clusters indicated that succinylation regulators might promote malignant progression of ccRCC by regulating the infiltration of immune cells and RNA N6-methyladenosine (m6A) methylation. Importantly, our data suggest that CPT1A and SIRT5 might up-regulate and down-regulate the expression of LRPPRC and EIF3B, respectively. Our study systematically analyzed the prognostic predictive values of four succinylation regulators and revealed their potential mechanisms in ccRCC aggressiveness. These data provide new insight into the understanding of succinylation modification and present clinical evidence for its role in ccRCC treatments. Frontiers Media S.A. 2021-02-18 /pmc/articles/PMC7935513/ /pubmed/33681201 http://dx.doi.org/10.3389/fcell.2021.622198 Text en Copyright © 2021 Lu, Che, Qu, Zheng, Yang, Bao, Li, Wang, Jin, Wang, Xiao, Qi and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Lu, Wenqing
Che, Xiaofang
Qu, Xiujuan
Zheng, Chunlei
Yang, Xianghong
Bao, Bowen
Li, Zhi
Wang, Duo
Jin, Yue
Wang, Yizhe
Xiao, Jiawen
Qi, Jianfei
Liu, Yunpeng
Succinylation Regulators Promote Clear Cell Renal Cell Carcinoma by Immune Regulation and RNA N6-Methyladenosine Methylation
title Succinylation Regulators Promote Clear Cell Renal Cell Carcinoma by Immune Regulation and RNA N6-Methyladenosine Methylation
title_full Succinylation Regulators Promote Clear Cell Renal Cell Carcinoma by Immune Regulation and RNA N6-Methyladenosine Methylation
title_fullStr Succinylation Regulators Promote Clear Cell Renal Cell Carcinoma by Immune Regulation and RNA N6-Methyladenosine Methylation
title_full_unstemmed Succinylation Regulators Promote Clear Cell Renal Cell Carcinoma by Immune Regulation and RNA N6-Methyladenosine Methylation
title_short Succinylation Regulators Promote Clear Cell Renal Cell Carcinoma by Immune Regulation and RNA N6-Methyladenosine Methylation
title_sort succinylation regulators promote clear cell renal cell carcinoma by immune regulation and rna n6-methyladenosine methylation
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935513/
https://www.ncbi.nlm.nih.gov/pubmed/33681201
http://dx.doi.org/10.3389/fcell.2021.622198
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