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Engineered yeast genomes accurately assembled from pure and mixed samples
Yeast whole genome sequencing (WGS) lacks end-to-end workflows that identify genetic engineering. Here we present Prymetime, a tool that assembles yeast plasmids and chromosomes and annotates genetic engineering sequences. It is a hybrid workflow—it uses short and long reads as inputs to perform sep...
Autores principales: | Collins, Joseph H., Keating, Kevin W., Jones, Trent R., Balaji, Shravani, Marsan, Celeste B., Çomo, Marina, Newlon, Zachary J., Mitchell, Tom, Bartley, Bryan, Adler, Aaron, Roehner, Nicholas, Young, Eric M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935868/ https://www.ncbi.nlm.nih.gov/pubmed/33674578 http://dx.doi.org/10.1038/s41467-021-21656-9 |
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