Single-cell measurement of plasmid copy number and promoter activity

Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inf...

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Detalles Bibliográficos
Autores principales: Shao, Bin, Rammohan, Jayan, Anderson, Daniel A., Alperovich, Nina, Ross, David, Voigt, Christopher A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935883/
https://www.ncbi.nlm.nih.gov/pubmed/33674569
http://dx.doi.org/10.1038/s41467-021-21734-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935883/
https://www.ncbi.nlm.nih.gov/pubmed/33674569
http://dx.doi.org/10.1038/s41467-021-21734-y

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