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Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP

Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automati...

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Autores principales: Bokelmann, Lukas, Nickel, Olaf, Maricic, Tomislav, Pääbo, Svante, Meyer, Matthias, Borte, Stephan, Riesenberg, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935920/
https://www.ncbi.nlm.nih.gov/pubmed/33674580
http://dx.doi.org/10.1038/s41467-021-21627-0
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author Bokelmann, Lukas
Nickel, Olaf
Maricic, Tomislav
Pääbo, Svante
Meyer, Matthias
Borte, Stephan
Riesenberg, Stephan
author_facet Bokelmann, Lukas
Nickel, Olaf
Maricic, Tomislav
Pääbo, Svante
Meyer, Matthias
Borte, Stephan
Riesenberg, Stephan
author_sort Bokelmann, Lukas
collection PubMed
description Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual.
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spelling pubmed-79359202021-03-21 Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP Bokelmann, Lukas Nickel, Olaf Maricic, Tomislav Pääbo, Svante Meyer, Matthias Borte, Stephan Riesenberg, Stephan Nat Commun Article Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual. Nature Publishing Group UK 2021-03-05 /pmc/articles/PMC7935920/ /pubmed/33674580 http://dx.doi.org/10.1038/s41467-021-21627-0 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bokelmann, Lukas
Nickel, Olaf
Maricic, Tomislav
Pääbo, Svante
Meyer, Matthias
Borte, Stephan
Riesenberg, Stephan
Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP
title Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP
title_full Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP
title_fullStr Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP
title_full_unstemmed Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP
title_short Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP
title_sort point-of-care bulk testing for sars-cov-2 by combining hybridization capture with improved colorimetric lamp
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935920/
https://www.ncbi.nlm.nih.gov/pubmed/33674580
http://dx.doi.org/10.1038/s41467-021-21627-0
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