Cargando…

Engineering subtilisin proteases that specifically degrade active RAS

We describe the design, kinetic properties, and structures of engineered subtilisin proteases that degrade the active form of RAS by cleaving a conserved sequence in switch 2. RAS is a signaling protein that, when mutated, drives a third of human cancers. To generate high specificity for the RAS tar...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chen, Yingwei, Toth, Eric A., Ruan, Biao, Choi, Eun Jung, Simmerman, Richard, Chen, Yihong, He, Yanan, Wang, Ruixue, Godoy-Ruiz, Raquel, King, Harlan, Custer, Gregory, Travis Gallagher, D., Rozak, David A., Solomon, Melani, Muro, Silvia, Weber, David J., Orban, John, Fuerst, Thomas R., Bryan, Philip N.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935941/ https://www.ncbi.nlm.nih.gov/pubmed/33674772 http://dx.doi.org/10.1038/s42003-021-01818-7

Ejemplares similares

Design and characterization of a protein fold switching network
por: Ruan, Biao, et al.
Publicado: (2023)

Production of subtilisin proteases in bacteria and yeast
por: Rozanov, A.S., et al.
Publicado: (2021)

Structure of a Switchable Subtilisin Complexed with a Substrate and with the Activator Azide
por: Gallagher, Travis, et al.
Publicado: (2009)

Molecular Determinants of Binding to the Plasmodium Subtilisin-like Protease 1
por: Fulle, Simone, et al.
Publicado: (2013)

Reversible switching between two common protein folds in a designed system using only temperature
por: Solomon, Tsega L., et al.
Publicado: (2023)

New insights into the evolution of subtilisin-like serine protease genes in Pezizomycotina
por: Li, Juan, et al.
Publicado: (2010)

The subtilisin-like protease SBT3 contributes to insect resistance in tomato
por: Meyer, Michael, et al.
Publicado: (2016)

Intracellular Delivery of Active Proteins by Polyphosphazene Polymers
por: Qamar, Bareera, et al.
Publicado: (2021)

Subtilisin-like proteases in plant–pathogen recognition and immune priming: a perspective
por: Figueiredo, Andreia, et al.
Publicado: (2014)

Biodegradation of Pine Processionary Caterpillar Silk Is Mediated by Elastase- and Subtilisin-like Proteases
por: Diez-Galán, Alba, et al.
Publicado: (2022)

Computational Design of Protein-Based Inhibitors of Plasmodium vivax Subtilisin-Like 1 Protease
por: Bastianelli, Giacomo, et al.
Publicado: (2014)

Maturation of subtilisin-like protease NbSLP1 from microsporidia Nosema bombycis
por: Wang, Rong, et al.
Publicado: (2022)

A malaria parasite subtilisin propeptide-like protein is a potent inhibitor of the egress protease SUB1
por: Tarr, Sarah J., et al.
Publicado: (2020)

Evolution of a subtilisin-like protease gene family in the grass endophytic fungus Epichloë festucae
por: Bryant, Michelle K, et al.
Publicado: (2009)

The malaria parasite egress protease SUB1 is a calcium-dependent redox switch subtilisin
por: Withers-Martinez, Chrislaine, et al.
Publicado: (2014)

Isolation and Identification of an Extracellular Subtilisin-Like Serine Protease Secreted by the Bat Pathogen Pseudogymnoascus destructans
por: Pannkuk, Evan L., et al.
Publicado: (2015)

The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea
por: Liu, Xinqiang, et al.
Publicado: (2020)

Identification of two subtilisin‐like serine proteases engaged in the degradation of recombinant proteins in Nicotiana benthamiana
por: Puchol Tarazona, Alejandro A., et al.
Publicado: (2020)

Genomic and exoproteomic analyses of cold‐ and alkaline‐adapted bacteria reveal an abundance of secreted subtilisin‐like proteases
por: Lylloff, Jeanette E., et al.
Publicado: (2016)

Corrigendum: The malaria parasite egress protease SUB1 is a calcium-dependent redox switch subtilisin
por: Withers-Martinez, Chrislaine, et al.
Publicado: (2014)

Mutational analysis of the pro‐peptide of a marine intracellular subtilisin protease supports its role in inhibition
por: Bjerga, Gro E. K., et al.
Publicado: (2018)

Genome-Wide Comprehensive Survey of the Subtilisin-Like Proteases Gene Family Associated With Rice Caryopsis Development
por: Zheng, Kaifeng, et al.
Publicado: (2022)

Activation of the Plasmodium Egress Effector Subtilisin-Like Protease 1 Is Mediated by Plasmepsin X Destruction of the Prodomain
por: Mukherjee, Sumit, et al.
Publicado: (2023)

Proteolytic processing of a precursor protein for a growth-promoting peptide by a subtilisin serine protease in Arabidopsis
por: Srivastava, Renu, et al.
Publicado: (2008)

Early Diverging Insect-Pathogenic Fungi of the Order Entomophthorales Possess Diverse and Unique Subtilisin-Like Serine Proteases
por: Arnesen, Jonathan A., et al.
Publicado: (2018)

Optimization of fibrinogenolytic activity of Solanum tuberosum subtilisin-like protease (StSBTc-3) by response surface methodology
por: Pepe, Alfonso, et al.
Publicado: (2019)

Subtilisin-like Pr1 proteases marking the evolution of pathogenicity in a wide-spectrum insect-pathogenic fungus
por: Gao, Ben-Jie, et al.
Publicado: (2020)

Recombinant Production and Characterization of an Extracellular Subtilisin-Like Serine Protease from Acinetobacter baumannii of Fermented Food Origin
por: Muhammed, Nur Syafiqah, et al.
Publicado: (2021)

Efficacy and safety profile of a subtilisin protease produced by fermentation in Bacillus licheniformis to be used as a feed additive
por: Cupi, Denisa, et al.
Publicado: (2022)

Role of the Subtilisin-like Serine Protease CJPRB from Cordyceps javanica in Eliciting an Immune Response in Hyphantria cunea
por: Wang, Wenxiu, et al.
Publicado: (2023)

The Subtilisin-Like Protease AprV2 Is Required for Virulence and Uses a Novel Disulphide-Tethered Exosite to Bind Substrates
por: Kennan, Ruth M., et al.
Publicado: (2010)

Insights into the role of electrostatics in temperature adaptation: a comparative study of psychrophilic, mesophilic, and thermophilic subtilisin-like serine proteases
por: Xia, Yuan-Ling, et al.
Publicado: (2018)

Safety assessment of subtilisin QK in rats
por: Xiao, Shuai, et al.
Publicado: (2021)

Delivering a RAS protease halts tumor growth
por: Vidimar, Vania, et al.
Publicado: (2020)

Mycosin-1, a subtilisin-like serine protease of Mycobacterium tuberculosis, is cell wall-associated and expressed during infection of macrophages
por: Dave, Joel A, et al.
Publicado: (2002)

Molecular Cloning and Immunochemical Characterization of a New Japanese Cedar Pollen Allergen Homologous to Plant Subtilisin-Like Serine Protease
por: Nour Ibrahim, Ahmed Ragaa, et al.
Publicado: (2010)

Subtilisin-like protease-1 secreted through type IV secretion system contributes to high virulence of Streptococcus suis 2
por: Yin, Supeng, et al.
Publicado: (2016)

Extracellular Production, Characterization, and Engineering of a Polyextremotolerant Subtilisin-Like Protease From Feather-Degrading Thermoactinomyces vulgaris Strain CDF
por: Ding, Yidi, et al.
Publicado: (2020)

Using a color-coded ambigraphic nucleic acid notation to visualize conserved palindromic motifs within and across genomes
por: Rozak, David A, et al.
Publicado: (2014)

Optimization of subtilisin production from Bacillus subtilis strain ZK3 and biological and molecular characterization of synthesized subtilisin capped nanoparticles
por: Shettar, Shreya S., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_trruts08b86bpq3c7k19f8rcf7