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Random and combinatorial mutagenesis for improved total production of secretory target protein in Escherichia coli

Signal peptides and secretory carrier proteins are commonly used to secrete heterologous recombinant protein in Gram-negative bacteria. The Escherichia coli osmotically-inducible protein Y (OsmY) is a carrier protein that secretes a target protein extracellularly, and we have previously applied it i...

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Detalles Bibliográficos
Autores principales:	Gonzalez-Perez, David, Ratcliffe, James, Tan, Shu Khan, Wong, Mary Chen May, Yee, Yi Pei, Nyabadza, Natsai, Xu, Jian-He, Wong, Tuck Seng, Tee, Kang Lan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935960/ https://www.ncbi.nlm.nih.gov/pubmed/33674702 http://dx.doi.org/10.1038/s41598-021-84859-6

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