A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers

The separation of biomarkers from blood is straightforward in most molecular biology laboratories. However, separation in resource-limited settings, allowing for the successful removal of biomarkers for diagnostic applications, is not always possible. The situation is further complicated by the need...

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Autores principales: Lenz, Kiersten D., Jakhar, Shailja, Chen, Jing W., Anderson, Aaron S., Purcell, Dylan C., Ishak, Mohammad O., Harris, Jennifer F., Akhadov, Leyla E., Kubicek-Sutherland, Jessica Z., Nath, Pulak, Mukundan, Harshini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935985/
https://www.ncbi.nlm.nih.gov/pubmed/33674653
http://dx.doi.org/10.1038/s41598-021-84353-z
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author Lenz, Kiersten D.
Jakhar, Shailja
Chen, Jing W.
Anderson, Aaron S.
Purcell, Dylan C.
Ishak, Mohammad O.
Harris, Jennifer F.
Akhadov, Leyla E.
Kubicek-Sutherland, Jessica Z.
Nath, Pulak
Mukundan, Harshini
author_facet Lenz, Kiersten D.
Jakhar, Shailja
Chen, Jing W.
Anderson, Aaron S.
Purcell, Dylan C.
Ishak, Mohammad O.
Harris, Jennifer F.
Akhadov, Leyla E.
Kubicek-Sutherland, Jessica Z.
Nath, Pulak
Mukundan, Harshini
author_sort Lenz, Kiersten D.
collection PubMed
description The separation of biomarkers from blood is straightforward in most molecular biology laboratories. However, separation in resource-limited settings, allowing for the successful removal of biomarkers for diagnostic applications, is not always possible. The situation is further complicated by the need to separate hydrophobic signatures such as lipids from blood. Herein, we present a microfluidic device capable of centrifugal separation of serum from blood at the point of need with a system that is compatible with biomarkers that are both hydrophilic and hydrophobic. The cross-flow filtration device separates serum from blood as efficiently as traditional methods and retains amphiphilic biomarkers in serum for detection.
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spelling pubmed-79359852021-03-08 A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers Lenz, Kiersten D. Jakhar, Shailja Chen, Jing W. Anderson, Aaron S. Purcell, Dylan C. Ishak, Mohammad O. Harris, Jennifer F. Akhadov, Leyla E. Kubicek-Sutherland, Jessica Z. Nath, Pulak Mukundan, Harshini Sci Rep Article The separation of biomarkers from blood is straightforward in most molecular biology laboratories. However, separation in resource-limited settings, allowing for the successful removal of biomarkers for diagnostic applications, is not always possible. The situation is further complicated by the need to separate hydrophobic signatures such as lipids from blood. Herein, we present a microfluidic device capable of centrifugal separation of serum from blood at the point of need with a system that is compatible with biomarkers that are both hydrophilic and hydrophobic. The cross-flow filtration device separates serum from blood as efficiently as traditional methods and retains amphiphilic biomarkers in serum for detection. Nature Publishing Group UK 2021-03-05 /pmc/articles/PMC7935985/ /pubmed/33674653 http://dx.doi.org/10.1038/s41598-021-84353-z Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lenz, Kiersten D.
Jakhar, Shailja
Chen, Jing W.
Anderson, Aaron S.
Purcell, Dylan C.
Ishak, Mohammad O.
Harris, Jennifer F.
Akhadov, Leyla E.
Kubicek-Sutherland, Jessica Z.
Nath, Pulak
Mukundan, Harshini
A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers
title A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers
title_full A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers
title_fullStr A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers
title_full_unstemmed A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers
title_short A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers
title_sort centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935985/
https://www.ncbi.nlm.nih.gov/pubmed/33674653
http://dx.doi.org/10.1038/s41598-021-84353-z
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