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Research Note: Characterization of monoclonal antibodies and development of sandwich ELISA for detecting chicken IL7

IL7 is a hematopoietic growth factor required for development and maintenance of lymphocytes including T cells, B cells, and natural killer cells. Recently, chicken IL7 (chIL7) has been cloned and studied in viral and parasite infection models. However, no monoclonal antibodies (mAb) that specifical...

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Autores principales: Panebra, Alfredo, Kim, Woo H., Hong, Yeong H., Lillehoj, Hyun S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936204/
https://www.ncbi.nlm.nih.gov/pubmed/33652529
http://dx.doi.org/10.1016/j.psj.2020.12.037
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author Panebra, Alfredo
Kim, Woo H.
Hong, Yeong H.
Lillehoj, Hyun S.
author_facet Panebra, Alfredo
Kim, Woo H.
Hong, Yeong H.
Lillehoj, Hyun S.
author_sort Panebra, Alfredo
collection PubMed
description IL7 is a hematopoietic growth factor required for development and maintenance of lymphocytes including T cells, B cells, and natural killer cells. Recently, chicken IL7 (chIL7) has been cloned and studied in viral and parasite infection models. However, no monoclonal antibodies (mAb) that specifically detect chIL7 have been developed so far. In this study, recombinant chIL7 that expressed for immunization and mAb against chIL7 were developed and characterized to assess their immunologic properties. Five mAb exhibiting specific binding to chIL7 were generated and investigated for their applicability by Western blot, ELISA, and neutralization assays. A sandwich ELISA mAb pair that enables the measurement of chIL7 protein levels in biological samples from Eimeria-infected chickens was identified and several mAb neutralized chicken primary thymocyte proliferation mediated by chIL7. The mAb developed in this study will be valuable reagents for fundamental and applied immunological studies in poultry.
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spelling pubmed-79362042021-03-15 Research Note: Characterization of monoclonal antibodies and development of sandwich ELISA for detecting chicken IL7 Panebra, Alfredo Kim, Woo H. Hong, Yeong H. Lillehoj, Hyun S. Poult Sci Immunology, Health and Disease IL7 is a hematopoietic growth factor required for development and maintenance of lymphocytes including T cells, B cells, and natural killer cells. Recently, chicken IL7 (chIL7) has been cloned and studied in viral and parasite infection models. However, no monoclonal antibodies (mAb) that specifically detect chIL7 have been developed so far. In this study, recombinant chIL7 that expressed for immunization and mAb against chIL7 were developed and characterized to assess their immunologic properties. Five mAb exhibiting specific binding to chIL7 were generated and investigated for their applicability by Western blot, ELISA, and neutralization assays. A sandwich ELISA mAb pair that enables the measurement of chIL7 protein levels in biological samples from Eimeria-infected chickens was identified and several mAb neutralized chicken primary thymocyte proliferation mediated by chIL7. The mAb developed in this study will be valuable reagents for fundamental and applied immunological studies in poultry. Elsevier 2021-01-05 /pmc/articles/PMC7936204/ /pubmed/33652529 http://dx.doi.org/10.1016/j.psj.2020.12.037 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Immunology, Health and Disease
Panebra, Alfredo
Kim, Woo H.
Hong, Yeong H.
Lillehoj, Hyun S.
Research Note: Characterization of monoclonal antibodies and development of sandwich ELISA for detecting chicken IL7
title Research Note: Characterization of monoclonal antibodies and development of sandwich ELISA for detecting chicken IL7
title_full Research Note: Characterization of monoclonal antibodies and development of sandwich ELISA for detecting chicken IL7
title_fullStr Research Note: Characterization of monoclonal antibodies and development of sandwich ELISA for detecting chicken IL7
title_full_unstemmed Research Note: Characterization of monoclonal antibodies and development of sandwich ELISA for detecting chicken IL7
title_short Research Note: Characterization of monoclonal antibodies and development of sandwich ELISA for detecting chicken IL7
title_sort research note: characterization of monoclonal antibodies and development of sandwich elisa for detecting chicken il7
topic Immunology, Health and Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936204/
https://www.ncbi.nlm.nih.gov/pubmed/33652529
http://dx.doi.org/10.1016/j.psj.2020.12.037
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