Cargando…
The bacterial community and metabolome dynamics and their interactions modulate fermentation process of whole crop corn silage prepared with or without inoculants
Multi‐omics approach was adopted to investigate the modulation of bacterial microbiota and metabolome as well as their interactions in whole crop corn ensiling systems by inoculating homofermentative Lactobacillus plantarum or heterofermentative Lactobacillus buchneri. Inoculations of the two differ...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936295/ https://www.ncbi.nlm.nih.gov/pubmed/32627363 http://dx.doi.org/10.1111/1751-7915.13623 |
_version_ | 1783661172366508032 |
---|---|
author | Xu, Dongmei Wang, Nian Rinne, Marketta Ke, Wencan Weinberg, Zwika G. Da, Mi Bai, Jie Zhang, Yixin Li, Fuhou Guo, Xusheng |
author_facet | Xu, Dongmei Wang, Nian Rinne, Marketta Ke, Wencan Weinberg, Zwika G. Da, Mi Bai, Jie Zhang, Yixin Li, Fuhou Guo, Xusheng |
author_sort | Xu, Dongmei |
collection | PubMed |
description | Multi‐omics approach was adopted to investigate the modulation of bacterial microbiota and metabolome as well as their interactions in whole crop corn ensiling systems by inoculating homofermentative Lactobacillus plantarum or heterofermentative Lactobacillus buchneri. Inoculations of the two different inoculants resulted in substantial differences in microbial community and metabolic composition as well as their dynamics in ensiled corn. Inoculants also altered the correlations of microbiota in different manners, and various keystone species were identified in corn silages with different treatments. Many metabolites with biofunctional activities like bacteriostatic, antioxidant, central nervous system inhibitory and anti‐inflammatory were found in the present silage. A constitutive difference in microbiota dynamics was found for several pathways, which were upregulated by specific taxa in middle stage of fermentation, and widespread associations between metabolites with biofunctions and the species of lactic acid bacteria dominated in silage were observed. Multiple microbial and metabolic structures and dynamics were correlated and affected the fermentation process of the corn ensiling systems. Results of the current study improve our understanding of the complicated biological process underlying silage fermentation and provide a framework to re‐evaluate silages with biofunctions, which may contribute to target‐based regulation methods to produce functional silage for animal production. |
format | Online Article Text |
id | pubmed-7936295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79362952021-03-16 The bacterial community and metabolome dynamics and their interactions modulate fermentation process of whole crop corn silage prepared with or without inoculants Xu, Dongmei Wang, Nian Rinne, Marketta Ke, Wencan Weinberg, Zwika G. Da, Mi Bai, Jie Zhang, Yixin Li, Fuhou Guo, Xusheng Microb Biotechnol Special Issue Articles Multi‐omics approach was adopted to investigate the modulation of bacterial microbiota and metabolome as well as their interactions in whole crop corn ensiling systems by inoculating homofermentative Lactobacillus plantarum or heterofermentative Lactobacillus buchneri. Inoculations of the two different inoculants resulted in substantial differences in microbial community and metabolic composition as well as their dynamics in ensiled corn. Inoculants also altered the correlations of microbiota in different manners, and various keystone species were identified in corn silages with different treatments. Many metabolites with biofunctional activities like bacteriostatic, antioxidant, central nervous system inhibitory and anti‐inflammatory were found in the present silage. A constitutive difference in microbiota dynamics was found for several pathways, which were upregulated by specific taxa in middle stage of fermentation, and widespread associations between metabolites with biofunctions and the species of lactic acid bacteria dominated in silage were observed. Multiple microbial and metabolic structures and dynamics were correlated and affected the fermentation process of the corn ensiling systems. Results of the current study improve our understanding of the complicated biological process underlying silage fermentation and provide a framework to re‐evaluate silages with biofunctions, which may contribute to target‐based regulation methods to produce functional silage for animal production. John Wiley and Sons Inc. 2020-07-06 /pmc/articles/PMC7936295/ /pubmed/32627363 http://dx.doi.org/10.1111/1751-7915.13623 Text en © 2020 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Special Issue Articles Xu, Dongmei Wang, Nian Rinne, Marketta Ke, Wencan Weinberg, Zwika G. Da, Mi Bai, Jie Zhang, Yixin Li, Fuhou Guo, Xusheng The bacterial community and metabolome dynamics and their interactions modulate fermentation process of whole crop corn silage prepared with or without inoculants |
title | The bacterial community and metabolome dynamics and their interactions modulate fermentation process of whole crop corn silage prepared with or without inoculants |
title_full | The bacterial community and metabolome dynamics and their interactions modulate fermentation process of whole crop corn silage prepared with or without inoculants |
title_fullStr | The bacterial community and metabolome dynamics and their interactions modulate fermentation process of whole crop corn silage prepared with or without inoculants |
title_full_unstemmed | The bacterial community and metabolome dynamics and their interactions modulate fermentation process of whole crop corn silage prepared with or without inoculants |
title_short | The bacterial community and metabolome dynamics and their interactions modulate fermentation process of whole crop corn silage prepared with or without inoculants |
title_sort | bacterial community and metabolome dynamics and their interactions modulate fermentation process of whole crop corn silage prepared with or without inoculants |
topic | Special Issue Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936295/ https://www.ncbi.nlm.nih.gov/pubmed/32627363 http://dx.doi.org/10.1111/1751-7915.13623 |
work_keys_str_mv | AT xudongmei thebacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT wangnian thebacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT rinnemarketta thebacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT kewencan thebacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT weinbergzwikag thebacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT dami thebacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT baijie thebacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT zhangyixin thebacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT lifuhou thebacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT guoxusheng thebacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT xudongmei bacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT wangnian bacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT rinnemarketta bacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT kewencan bacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT weinbergzwikag bacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT dami bacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT baijie bacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT zhangyixin bacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT lifuhou bacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants AT guoxusheng bacterialcommunityandmetabolomedynamicsandtheirinteractionsmodulatefermentationprocessofwholecropcornsilagepreparedwithorwithoutinoculants |