The effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells

We aimed to find the effect of caffeic acid phenethyl ester (CAPE) on the expression profiles of cell cycle control genes in breast cancer cell line (MCF-7). The cytotoxic effect of CAPE on MCF-7 cell line was found with an XTT analysis. Total RNA was isolated from the cells exposed to IC(50) dose a...

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Autores principales: Balc-Okcanoğlu, Tuğçe, Yilma-Susluer, Sunde, Kayabasi, Cagla, Ozme-Yelken, Besra, Biray-Avci, Cigir, Gunduz, Cumhur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shiraz University 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936384/
https://www.ncbi.nlm.nih.gov/pubmed/33681396
http://dx.doi.org/10.22099/mbrc.2020.38811.1563
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author Balc-Okcanoğlu, Tuğçe
Yilma-Susluer, Sunde
Kayabasi, Cagla
Ozme-Yelken, Besra
Biray-Avci, Cigir
Gunduz, Cumhur
author_facet Balc-Okcanoğlu, Tuğçe
Yilma-Susluer, Sunde
Kayabasi, Cagla
Ozme-Yelken, Besra
Biray-Avci, Cigir
Gunduz, Cumhur
author_sort Balc-Okcanoğlu, Tuğçe
collection PubMed
description We aimed to find the effect of caffeic acid phenethyl ester (CAPE) on the expression profiles of cell cycle control genes in breast cancer cell line (MCF-7). The cytotoxic effect of CAPE on MCF-7 cell line was found with an XTT analysis. Total RNA was isolated from the cells exposed to IC(50) dose and untreated control cells. Expressions of genes related to cell cycle control (CCND2, RB1, ATM, CDC34, CDK5RAP1) were evaluated by qRT-PCR by the LightCycler 480 System (Roche). GAPDH and ACTB housekeeping genes were used for the normalization of gene expressions. IC(50) value of CAPE in MCF-7 cells was calculated as 75µM. It was shown that IC(50 )dose of CAPE induced significant upregulation in expressions of cell cycle control genes, compared to control cells. CAPE increases the expression of genes that are important in cell cycle control, suggesting that this component can be used as an effective chemopreventive agent in breast cancer cells.
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spelling pubmed-79363842021-03-06 The effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells Balc-Okcanoğlu, Tuğçe Yilma-Susluer, Sunde Kayabasi, Cagla Ozme-Yelken, Besra Biray-Avci, Cigir Gunduz, Cumhur Mol Biol Res Commun Short Communication We aimed to find the effect of caffeic acid phenethyl ester (CAPE) on the expression profiles of cell cycle control genes in breast cancer cell line (MCF-7). The cytotoxic effect of CAPE on MCF-7 cell line was found with an XTT analysis. Total RNA was isolated from the cells exposed to IC(50) dose and untreated control cells. Expressions of genes related to cell cycle control (CCND2, RB1, ATM, CDC34, CDK5RAP1) were evaluated by qRT-PCR by the LightCycler 480 System (Roche). GAPDH and ACTB housekeeping genes were used for the normalization of gene expressions. IC(50) value of CAPE in MCF-7 cells was calculated as 75µM. It was shown that IC(50 )dose of CAPE induced significant upregulation in expressions of cell cycle control genes, compared to control cells. CAPE increases the expression of genes that are important in cell cycle control, suggesting that this component can be used as an effective chemopreventive agent in breast cancer cells. Shiraz University 2021-03 /pmc/articles/PMC7936384/ /pubmed/33681396 http://dx.doi.org/10.22099/mbrc.2020.38811.1563 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Balc-Okcanoğlu, Tuğçe
Yilma-Susluer, Sunde
Kayabasi, Cagla
Ozme-Yelken, Besra
Biray-Avci, Cigir
Gunduz, Cumhur
The effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells
title The effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells
title_full The effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells
title_fullStr The effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells
title_full_unstemmed The effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells
title_short The effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells
title_sort effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936384/
https://www.ncbi.nlm.nih.gov/pubmed/33681396
http://dx.doi.org/10.22099/mbrc.2020.38811.1563
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