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Oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3A gene (HTR3A) and electrolyte imbalances in brain of male Wistar rats

The gene expression of serotonin 5-hydroxytryptamine receptor 3A (receptor 3A:HTR3A) as well as the concentration of electrolytes in male Wistar rats after administration of graded doses of marijuana extract was investigated. Twelve groups (3 control and 9 test groups) of 6 animals each were daily e...

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Autores principales: Taiwo, Odunayo Anthonia, Dosumu, Oluwatosin Adebisi, Ugbaja, Regina Ngozi, Rotimi, Solomon Oladapo, Owolabi, Oluwafemi Paul, Ojo, Oluwafemi Adeleke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shiraz University 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936388/
https://www.ncbi.nlm.nih.gov/pubmed/33681392
http://dx.doi.org/10.22099/mbrc.2020.38601.1557
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author Taiwo, Odunayo Anthonia
Dosumu, Oluwatosin Adebisi
Ugbaja, Regina Ngozi
Rotimi, Solomon Oladapo
Owolabi, Oluwafemi Paul
Ojo, Oluwafemi Adeleke
author_facet Taiwo, Odunayo Anthonia
Dosumu, Oluwatosin Adebisi
Ugbaja, Regina Ngozi
Rotimi, Solomon Oladapo
Owolabi, Oluwafemi Paul
Ojo, Oluwafemi Adeleke
author_sort Taiwo, Odunayo Anthonia
collection PubMed
description The gene expression of serotonin 5-hydroxytryptamine receptor 3A (receptor 3A:HTR3A) as well as the concentration of electrolytes in male Wistar rats after administration of graded doses of marijuana extract was investigated. Twelve groups (3 control and 9 test groups) of 6 animals each were daily exposed to 12.5, 25 and 50 mg/kg b.w doses of petroleum ether extract of marijuana for 4, 8 and 12 weeks. The expressions of the gene were obtained using reverse transcriptase-polymerase chain reaction (RT-PCR) while electrolytes concentrations were determined. An upregulation of over 90% was observed in the expression of HTR3A after exposure to the highest dose throughout the exposure period. There was significant increase in the plasma potassium concentration at all doses while there was a decrease in the brain only at 50 mg/kg dose throughout the exposure period. Sodium concentration in the brain was not affected by the doses over the period of exposure but plasma concentration decreased significantly. All the doses of marijuana extract significantly increased calcium concentration in the brain after prolonged exposure but the plasma concentration remained unchanged. This suggests that different doses of marijuana extract alter the expression of serotonin receptor and electrolyte concentrations over a period of time with possible neurological consequences.
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spelling pubmed-79363882021-03-06 Oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3A gene (HTR3A) and electrolyte imbalances in brain of male Wistar rats Taiwo, Odunayo Anthonia Dosumu, Oluwatosin Adebisi Ugbaja, Regina Ngozi Rotimi, Solomon Oladapo Owolabi, Oluwafemi Paul Ojo, Oluwafemi Adeleke Mol Biol Res Commun Original Article The gene expression of serotonin 5-hydroxytryptamine receptor 3A (receptor 3A:HTR3A) as well as the concentration of electrolytes in male Wistar rats after administration of graded doses of marijuana extract was investigated. Twelve groups (3 control and 9 test groups) of 6 animals each were daily exposed to 12.5, 25 and 50 mg/kg b.w doses of petroleum ether extract of marijuana for 4, 8 and 12 weeks. The expressions of the gene were obtained using reverse transcriptase-polymerase chain reaction (RT-PCR) while electrolytes concentrations were determined. An upregulation of over 90% was observed in the expression of HTR3A after exposure to the highest dose throughout the exposure period. There was significant increase in the plasma potassium concentration at all doses while there was a decrease in the brain only at 50 mg/kg dose throughout the exposure period. Sodium concentration in the brain was not affected by the doses over the period of exposure but plasma concentration decreased significantly. All the doses of marijuana extract significantly increased calcium concentration in the brain after prolonged exposure but the plasma concentration remained unchanged. This suggests that different doses of marijuana extract alter the expression of serotonin receptor and electrolyte concentrations over a period of time with possible neurological consequences. Shiraz University 2021-03 /pmc/articles/PMC7936388/ /pubmed/33681392 http://dx.doi.org/10.22099/mbrc.2020.38601.1557 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Taiwo, Odunayo Anthonia
Dosumu, Oluwatosin Adebisi
Ugbaja, Regina Ngozi
Rotimi, Solomon Oladapo
Owolabi, Oluwafemi Paul
Ojo, Oluwafemi Adeleke
Oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3A gene (HTR3A) and electrolyte imbalances in brain of male Wistar rats
title Oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3A gene (HTR3A) and electrolyte imbalances in brain of male Wistar rats
title_full Oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3A gene (HTR3A) and electrolyte imbalances in brain of male Wistar rats
title_fullStr Oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3A gene (HTR3A) and electrolyte imbalances in brain of male Wistar rats
title_full_unstemmed Oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3A gene (HTR3A) and electrolyte imbalances in brain of male Wistar rats
title_short Oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3A gene (HTR3A) and electrolyte imbalances in brain of male Wistar rats
title_sort oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3a gene (htr3a) and electrolyte imbalances in brain of male wistar rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936388/
https://www.ncbi.nlm.nih.gov/pubmed/33681392
http://dx.doi.org/10.22099/mbrc.2020.38601.1557
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