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Influence of water availability and temperature on estimates of microbial extracellular enzyme activity

Soils are highly heterogeneous and support highly diverse microbial communities. Microbial extracellular enzymes breakdown complex polymers into small assimilable molecules representing the limiting step of soil organic matter mineralization. This process occurs on to soil particles although current...

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Detalles Bibliográficos
Autores principales: Gomez, Enrique J., Delgado, Jose A., Gonzalez, Juan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936561/
https://www.ncbi.nlm.nih.gov/pubmed/33717705
http://dx.doi.org/10.7717/peerj.10994
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author Gomez, Enrique J.
Delgado, Jose A.
Gonzalez, Juan M.
author_facet Gomez, Enrique J.
Delgado, Jose A.
Gonzalez, Juan M.
author_sort Gomez, Enrique J.
collection PubMed
description Soils are highly heterogeneous and support highly diverse microbial communities. Microbial extracellular enzymes breakdown complex polymers into small assimilable molecules representing the limiting step of soil organic matter mineralization. This process occurs on to soil particles although currently it is typically estimated in laboratory aqueous solutions. Herein, estimates of microbial extracellular enzyme activity were obtained over a broad range of temperatures and water availabilities frequently observed at soil upper layers. A Pseudomonas strain presented optimum extracellular enzyme activities at high water activity whereas a desiccation resistant bacterium (Deinococcus) and a soil thermophilic isolate (Parageobacillus) showed optimum extracellular enzyme activity under dried (i.e., water activities ranging 0.5–0.8) rather that wet conditions. Different unamended soils presented a distinctive response of extracellular enzyme activity as a function of temperature and water availability. This study presents a procedure to obtain realistic estimates of microbial extracellular enzyme activity under natural soil conditions of extreme water availability and temperature. Improving estimates of microbial extracellular enzyme activity contribute to better understand the role of microorganisms in soils.
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spelling pubmed-79365612021-03-12 Influence of water availability and temperature on estimates of microbial extracellular enzyme activity Gomez, Enrique J. Delgado, Jose A. Gonzalez, Juan M. PeerJ Ecology Soils are highly heterogeneous and support highly diverse microbial communities. Microbial extracellular enzymes breakdown complex polymers into small assimilable molecules representing the limiting step of soil organic matter mineralization. This process occurs on to soil particles although currently it is typically estimated in laboratory aqueous solutions. Herein, estimates of microbial extracellular enzyme activity were obtained over a broad range of temperatures and water availabilities frequently observed at soil upper layers. A Pseudomonas strain presented optimum extracellular enzyme activities at high water activity whereas a desiccation resistant bacterium (Deinococcus) and a soil thermophilic isolate (Parageobacillus) showed optimum extracellular enzyme activity under dried (i.e., water activities ranging 0.5–0.8) rather that wet conditions. Different unamended soils presented a distinctive response of extracellular enzyme activity as a function of temperature and water availability. This study presents a procedure to obtain realistic estimates of microbial extracellular enzyme activity under natural soil conditions of extreme water availability and temperature. Improving estimates of microbial extracellular enzyme activity contribute to better understand the role of microorganisms in soils. PeerJ Inc. 2021-03-03 /pmc/articles/PMC7936561/ /pubmed/33717705 http://dx.doi.org/10.7717/peerj.10994 Text en © 2021 Gomez et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Ecology
Gomez, Enrique J.
Delgado, Jose A.
Gonzalez, Juan M.
Influence of water availability and temperature on estimates of microbial extracellular enzyme activity
title Influence of water availability and temperature on estimates of microbial extracellular enzyme activity
title_full Influence of water availability and temperature on estimates of microbial extracellular enzyme activity
title_fullStr Influence of water availability and temperature on estimates of microbial extracellular enzyme activity
title_full_unstemmed Influence of water availability and temperature on estimates of microbial extracellular enzyme activity
title_short Influence of water availability and temperature on estimates of microbial extracellular enzyme activity
title_sort influence of water availability and temperature on estimates of microbial extracellular enzyme activity
topic Ecology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936561/
https://www.ncbi.nlm.nih.gov/pubmed/33717705
http://dx.doi.org/10.7717/peerj.10994
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