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The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication
Rice stripe virus (RSV) transmitted by the small brown planthopper causes severe rice yield losses in Asian countries. Although viral nuclear entry promotes viral replication in host cells, whether this phenomenon occurs in vector cells remains unknown. Therefore, in this study, we systematically ev...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Higher Education Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936609/ https://www.ncbi.nlm.nih.gov/pubmed/33675514 http://dx.doi.org/10.1007/s13238-021-00822-1 |
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author | Zhao, Wan Zhu, Junjie Lu, Hong Zhu, Jiaming Jiang, Fei Wang, Wei Luo, Lan Kang, Le Cui, Feng |
author_facet | Zhao, Wan Zhu, Junjie Lu, Hong Zhu, Jiaming Jiang, Fei Wang, Wei Luo, Lan Kang, Le Cui, Feng |
author_sort | Zhao, Wan |
collection | PubMed |
description | Rice stripe virus (RSV) transmitted by the small brown planthopper causes severe rice yield losses in Asian countries. Although viral nuclear entry promotes viral replication in host cells, whether this phenomenon occurs in vector cells remains unknown. Therefore, in this study, we systematically evaluated the presence and roles of RSV in the nuclei of vector insect cells. We observed that the nucleocapsid protein (NP) and viral genomic RNAs were partially transported into vector cell nuclei by utilizing the importin α nuclear transport system. When blocking NP nuclear localization, cytoplasmic RSV accumulation significantly increased. In the vector cell nuclei, NP bound the transcription factor YY1 and affected its positive regulation to FAIM. Subsequently, decreased FAIM expression triggered an antiviral caspase-dependent apoptotic reaction. Our results reveal that viral nuclear entry induces completely different immune effects in vector and host cells, providing new insights into the balance between viral load and the immunity pressure in vector insects. SUPPLEMENTARY INFORMATION: The online version of this article (10.1007/s13238-021-00822-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7936609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Higher Education Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-79366092021-03-08 The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication Zhao, Wan Zhu, Junjie Lu, Hong Zhu, Jiaming Jiang, Fei Wang, Wei Luo, Lan Kang, Le Cui, Feng Protein Cell Research Article Rice stripe virus (RSV) transmitted by the small brown planthopper causes severe rice yield losses in Asian countries. Although viral nuclear entry promotes viral replication in host cells, whether this phenomenon occurs in vector cells remains unknown. Therefore, in this study, we systematically evaluated the presence and roles of RSV in the nuclei of vector insect cells. We observed that the nucleocapsid protein (NP) and viral genomic RNAs were partially transported into vector cell nuclei by utilizing the importin α nuclear transport system. When blocking NP nuclear localization, cytoplasmic RSV accumulation significantly increased. In the vector cell nuclei, NP bound the transcription factor YY1 and affected its positive regulation to FAIM. Subsequently, decreased FAIM expression triggered an antiviral caspase-dependent apoptotic reaction. Our results reveal that viral nuclear entry induces completely different immune effects in vector and host cells, providing new insights into the balance between viral load and the immunity pressure in vector insects. SUPPLEMENTARY INFORMATION: The online version of this article (10.1007/s13238-021-00822-1) contains supplementary material, which is available to authorized users. Higher Education Press 2021-03-06 2022-05 /pmc/articles/PMC7936609/ /pubmed/33675514 http://dx.doi.org/10.1007/s13238-021-00822-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Zhao, Wan Zhu, Junjie Lu, Hong Zhu, Jiaming Jiang, Fei Wang, Wei Luo, Lan Kang, Le Cui, Feng The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication |
title | The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication |
title_full | The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication |
title_fullStr | The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication |
title_full_unstemmed | The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication |
title_short | The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication |
title_sort | nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936609/ https://www.ncbi.nlm.nih.gov/pubmed/33675514 http://dx.doi.org/10.1007/s13238-021-00822-1 |
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